Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11270 and RR42_RS11275 are separated by 80 nucleotides RR42_RS11275 and RR42_RS11280 are separated by 382 nucleotides RR42_RS11280 and RR42_RS11285 are separated by 112 nucleotides
RR42_RS11270: RR42_RS11270 - aconitate hydratase, at 2,409,411 to 2,412,008
_RS11270
RR42_RS11275: RR42_RS11275 - 3-methylitaconate isomerase, at 2,412,089 to 2,413,279
_RS11275
RR42_RS11280: RR42_RS11280 - phenylacetic acid degradation protein, at 2,413,662 to 2,414,090
_RS11280
RR42_RS11285: RR42_RS11285 - thioesterase, at 2,414,203 to 2,414,640
_RS11285
Position (kb)
2412
2413
2414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2411.155 kb on - strand, within RR42_RS11270 at 2411.208 kb on + strand, within RR42_RS11270 at 2411.233 kb on - strand, within RR42_RS11270 at 2411.233 kb on - strand, within RR42_RS11270 at 2411.383 kb on - strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.817 kb on + strand at 2411.940 kb on + strand at 2411.941 kb on - strand at 2411.941 kb on - strand at 2411.941 kb on - strand at 2412.006 kb on + strand at 2412.049 kb on - strand at 2412.058 kb on + strand at 2412.059 kb on - strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.249 kb on - strand, within RR42_RS11275 at 2412.534 kb on + strand, within RR42_RS11275 at 2412.534 kb on + strand, within RR42_RS11275 at 2412.535 kb on - strand, within RR42_RS11275 at 2412.535 kb on - strand, within RR42_RS11275 at 2412.886 kb on + strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.950 kb on + strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2413.353 kb on - strand at 2413.360 kb on + strand at 2413.360 kb on + strand at 2413.361 kb on - strand at 2413.361 kb on - strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.369 kb on - strand at 2413.609 kb on + strand at 2413.610 kb on - strand at 2413.620 kb on + strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.867 kb on + strand, within RR42_RS11280 at 2413.966 kb on + strand, within RR42_RS11280 at 2414.096 kb on - strand at 2414.154 kb on - strand at 2414.172 kb on - strand at 2414.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,411,155 - RR42_RS11270 0.67 +2.0 2,411,208 + RR42_RS11270 0.69 -2.9 2,411,233 - RR42_RS11270 0.70 -0.8 2,411,233 - RR42_RS11270 0.70 -1.2 2,411,383 - RR42_RS11270 0.76 -3.6 2,411,589 + RR42_RS11270 0.84 -2.8 2,411,589 + RR42_RS11270 0.84 -0.6 2,411,589 + RR42_RS11270 0.84 -2.8 2,411,589 + RR42_RS11270 0.84 -3.2 2,411,589 + RR42_RS11270 0.84 -1.5 2,411,589 + RR42_RS11270 0.84 -1.8 2,411,590 - RR42_RS11270 0.84 -1.8 2,411,590 - RR42_RS11270 0.84 -0.5 2,411,590 - RR42_RS11270 0.84 -2.4 2,411,817 + +0.2 2,411,940 + +1.2 2,411,941 - -1.1 2,411,941 - +0.3 2,411,941 - -3.1 2,412,006 + -1.6 2,412,049 - +0.8 2,412,058 + +0.0 2,412,059 - -2.2 2,412,125 + -0.8 2,412,125 + +2.2 2,412,125 + -1.5 2,412,125 + -1.4 2,412,126 - -2.1 2,412,126 - +1.4 2,412,126 - -0.8 2,412,126 - -1.2 2,412,126 - -0.4 2,412,126 - -2.8 2,412,249 - RR42_RS11275 0.13 -1.2 2,412,534 + RR42_RS11275 0.37 -2.4 2,412,534 + RR42_RS11275 0.37 -0.4 2,412,535 - RR42_RS11275 0.37 -1.6 2,412,535 - RR42_RS11275 0.37 -1.8 2,412,886 + RR42_RS11275 0.67 -2.5 2,412,887 - RR42_RS11275 0.67 -2.7 2,412,887 - RR42_RS11275 0.67 -0.1 2,412,950 + RR42_RS11275 0.72 -0.6 2,412,951 - RR42_RS11275 0.72 -0.8 2,412,951 - RR42_RS11275 0.72 -0.0 2,412,951 - RR42_RS11275 0.72 -0.4 2,413,353 - -1.2 2,413,360 + -0.2 2,413,360 + -0.0 2,413,361 - +1.0 2,413,361 - +0.1 2,413,368 + +2.2 2,413,368 + -0.0 2,413,368 + -0.1 2,413,368 + -3.1 2,413,369 - -1.3 2,413,609 + +0.9 2,413,610 - -0.4 2,413,620 + +1.1 2,413,621 - +0.5 2,413,621 - -1.6 2,413,621 - +0.1 2,413,867 + RR42_RS11280 0.48 +0.6 2,413,966 + RR42_RS11280 0.71 -1.7 2,414,096 - -2.1 2,414,154 - +1.2 2,414,172 - -0.6 2,414,172 - -1.7
Or see this region's nucleotide sequence