Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10320 and RR42_RS10325 are separated by 306 nucleotides RR42_RS10325 and RR42_RS10330 are separated by 21 nucleotides
RR42_RS10320: RR42_RS10320 - short-chain dehydrogenase, at 2,207,244 to 2,208,011
_RS10320
RR42_RS10325: RR42_RS10325 - 5-oxoprolinase, at 2,208,318 to 2,210,390
_RS10325
RR42_RS10330: RR42_RS10330 - hydantoinase, at 2,210,412 to 2,212,199
_RS10330
Position (kb)
2208
2209
2210
2211 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2207.450 kb on + strand, within RR42_RS10320 at 2207.451 kb on - strand, within RR42_RS10320 at 2207.451 kb on - strand, within RR42_RS10320 at 2207.484 kb on + strand, within RR42_RS10320 at 2207.548 kb on + strand, within RR42_RS10320 at 2207.549 kb on - strand, within RR42_RS10320 at 2207.557 kb on + strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.675 kb on + strand, within RR42_RS10320 at 2207.676 kb on - strand, within RR42_RS10320 at 2207.895 kb on - strand, within RR42_RS10320 at 2207.895 kb on - strand, within RR42_RS10320 at 2207.989 kb on - strand at 2208.157 kb on + strand at 2208.180 kb on + strand at 2208.180 kb on + strand at 2208.317 kb on + strand at 2208.318 kb on - strand at 2208.335 kb on + strand at 2208.344 kb on + strand at 2208.345 kb on - strand at 2208.389 kb on + strand at 2208.389 kb on + strand at 2208.390 kb on - strand at 2208.390 kb on - strand at 2208.509 kb on + strand at 2208.510 kb on - strand at 2208.510 kb on - strand at 2208.513 kb on + strand at 2208.514 kb on - strand at 2208.514 kb on - strand at 2208.978 kb on + strand, within RR42_RS10325 at 2208.978 kb on + strand, within RR42_RS10325 at 2208.978 kb on + strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.980 kb on + strand, within RR42_RS10325 at 2208.980 kb on + strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2209.138 kb on - strand, within RR42_RS10325 at 2209.200 kb on + strand, within RR42_RS10325 at 2209.200 kb on + strand, within RR42_RS10325 at 2209.201 kb on - strand, within RR42_RS10325 at 2209.201 kb on - strand, within RR42_RS10325 at 2209.201 kb on - strand, within RR42_RS10325 at 2209.304 kb on + strand, within RR42_RS10325 at 2209.305 kb on - strand, within RR42_RS10325 at 2209.305 kb on - strand, within RR42_RS10325 at 2209.461 kb on + strand, within RR42_RS10325 at 2209.462 kb on - strand, within RR42_RS10325 at 2209.528 kb on + strand, within RR42_RS10325 at 2209.528 kb on + strand, within RR42_RS10325 at 2209.608 kb on - strand, within RR42_RS10325 at 2209.823 kb on + strand, within RR42_RS10325 at 2210.006 kb on + strand, within RR42_RS10325 at 2210.388 kb on + strand at 2210.540 kb on + strand at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.862 kb on + strand, within RR42_RS10330 at 2210.863 kb on - strand, within RR42_RS10330 at 2211.350 kb on + strand, within RR42_RS10330
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,207,450 + RR42_RS10320 0.27 -0.7 2,207,451 - RR42_RS10320 0.27 +0.9 2,207,451 - RR42_RS10320 0.27 -0.3 2,207,484 + RR42_RS10320 0.31 +0.6 2,207,548 + RR42_RS10320 0.40 +0.5 2,207,549 - RR42_RS10320 0.40 -0.1 2,207,557 + RR42_RS10320 0.41 -0.3 2,207,558 - RR42_RS10320 0.41 -0.9 2,207,558 - RR42_RS10320 0.41 -1.3 2,207,558 - RR42_RS10320 0.41 -0.3 2,207,675 + RR42_RS10320 0.56 +0.4 2,207,676 - RR42_RS10320 0.56 +1.0 2,207,895 - RR42_RS10320 0.85 +0.7 2,207,895 - RR42_RS10320 0.85 +0.4 2,207,989 - +0.2 2,208,157 + -2.5 2,208,180 + -1.3 2,208,180 + -1.3 2,208,317 + +1.7 2,208,318 - +0.2 2,208,335 + +1.4 2,208,344 + +0.3 2,208,345 - +1.9 2,208,389 + -0.3 2,208,389 + -1.3 2,208,390 - +1.8 2,208,390 - -1.3 2,208,509 + -2.1 2,208,510 - +1.1 2,208,510 - -0.2 2,208,513 + -1.9 2,208,514 - -1.7 2,208,514 - -1.0 2,208,978 + RR42_RS10325 0.32 -0.6 2,208,978 + RR42_RS10325 0.32 +0.5 2,208,978 + RR42_RS10325 0.32 +1.4 2,208,979 - RR42_RS10325 0.32 +1.2 2,208,979 - RR42_RS10325 0.32 +0.1 2,208,979 - RR42_RS10325 0.32 -1.7 2,208,979 - RR42_RS10325 0.32 -0.2 2,208,980 + RR42_RS10325 0.32 +0.4 2,208,980 + RR42_RS10325 0.32 +0.2 2,208,981 - RR42_RS10325 0.32 +0.5 2,208,981 - RR42_RS10325 0.32 +2.2 2,208,981 - RR42_RS10325 0.32 +1.0 2,208,981 - RR42_RS10325 0.32 -1.8 2,208,981 - RR42_RS10325 0.32 -0.3 2,208,981 - RR42_RS10325 0.32 -0.5 2,209,138 - RR42_RS10325 0.40 -0.9 2,209,200 + RR42_RS10325 0.43 +0.6 2,209,200 + RR42_RS10325 0.43 -0.2 2,209,201 - RR42_RS10325 0.43 -1.9 2,209,201 - RR42_RS10325 0.43 +0.6 2,209,201 - RR42_RS10325 0.43 +0.3 2,209,304 + RR42_RS10325 0.48 -0.2 2,209,305 - RR42_RS10325 0.48 -1.3 2,209,305 - RR42_RS10325 0.48 +0.2 2,209,461 + RR42_RS10325 0.55 +1.3 2,209,462 - RR42_RS10325 0.55 +0.3 2,209,528 + RR42_RS10325 0.58 +2.0 2,209,528 + RR42_RS10325 0.58 +0.2 2,209,608 - RR42_RS10325 0.62 -3.0 2,209,823 + RR42_RS10325 0.73 -0.3 2,210,006 + RR42_RS10325 0.81 -0.8 2,210,388 + +0.2 2,210,540 + -0.3 2,210,838 + RR42_RS10330 0.24 +0.7 2,210,838 + RR42_RS10330 0.24 +0.4 2,210,838 + RR42_RS10330 0.24 -2.7 2,210,838 + RR42_RS10330 0.24 +2.2 2,210,838 + RR42_RS10330 0.24 +0.3 2,210,839 - RR42_RS10330 0.24 -0.2 2,210,839 - RR42_RS10330 0.24 -0.1 2,210,839 - RR42_RS10330 0.24 +0.8 2,210,839 - RR42_RS10330 0.24 -0.6 2,210,862 + RR42_RS10330 0.25 +0.4 2,210,863 - RR42_RS10330 0.25 +1.4 2,211,350 + RR42_RS10330 0.52 -1.8
Or see this region's nucleotide sequence