Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10250 and RR42_RS10255 are separated by 59 nucleotides RR42_RS10255 and RR42_RS10260 are separated by 31 nucleotides RR42_RS10260 and RR42_RS10265 are separated by 28 nucleotides
RR42_RS10250: RR42_RS10250 - enoyl-CoA hydratase, at 2,194,466 to 2,195,254
_RS10250
RR42_RS10255: RR42_RS10255 - gluconate 5-dehydrogenase, at 2,195,314 to 2,196,096
_RS10255
RR42_RS10260: RR42_RS10260 - acetyl-CoA acetyltransferase, at 2,196,128 to 2,197,303
_RS10260
RR42_RS10265: RR42_RS10265 - 3-hydroxyacyl-CoA dehydrogenase, at 2,197,332 to 2,199,410
_RS10265
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2195.464 kb on + strand, within RR42_RS10255 at 2195.465 kb on - strand, within RR42_RS10255 at 2195.465 kb on - strand, within RR42_RS10255 at 2195.466 kb on - strand, within RR42_RS10255 at 2195.601 kb on - strand, within RR42_RS10255 at 2195.601 kb on - strand, within RR42_RS10255 at 2195.601 kb on - strand, within RR42_RS10255 at 2195.675 kb on + strand, within RR42_RS10255 at 2195.836 kb on + strand, within RR42_RS10255 at 2196.172 kb on + strand at 2196.172 kb on + strand at 2196.173 kb on - strand at 2196.186 kb on - strand at 2196.638 kb on - strand, within RR42_RS10260 at 2196.638 kb on - strand, within RR42_RS10260 at 2196.809 kb on + strand, within RR42_RS10260 at 2196.810 kb on - strand, within RR42_RS10260 at 2196.870 kb on + strand, within RR42_RS10260 at 2197.459 kb on + strand at 2197.582 kb on + strand, within RR42_RS10265 at 2197.709 kb on - strand, within RR42_RS10265 at 2197.955 kb on - strand, within RR42_RS10265 at 2198.021 kb on + strand, within RR42_RS10265 at 2198.022 kb on - strand, within RR42_RS10265 at 2198.190 kb on - strand, within RR42_RS10265 at 2198.269 kb on + strand, within RR42_RS10265 at 2198.270 kb on - strand, within RR42_RS10265 at 2198.283 kb on + strand, within RR42_RS10265 at 2198.284 kb on - strand, within RR42_RS10265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,195,464 + RR42_RS10255 0.19 +0.6 2,195,465 - RR42_RS10255 0.19 -1.1 2,195,465 - RR42_RS10255 0.19 +0.4 2,195,466 - RR42_RS10255 0.19 -0.2 2,195,601 - RR42_RS10255 0.37 +1.9 2,195,601 - RR42_RS10255 0.37 +0.5 2,195,601 - RR42_RS10255 0.37 -0.2 2,195,675 + RR42_RS10255 0.46 +0.6 2,195,836 + RR42_RS10255 0.67 +0.1 2,196,172 + -2.2 2,196,172 + +1.0 2,196,173 - +0.2 2,196,186 - -0.9 2,196,638 - RR42_RS10260 0.43 -0.3 2,196,638 - RR42_RS10260 0.43 -2.2 2,196,809 + RR42_RS10260 0.58 -4.7 2,196,810 - RR42_RS10260 0.58 -1.4 2,196,870 + RR42_RS10260 0.63 +0.2 2,197,459 + -1.7 2,197,582 + RR42_RS10265 0.12 +0.8 2,197,709 - RR42_RS10265 0.18 -2.2 2,197,955 - RR42_RS10265 0.30 +0.9 2,198,021 + RR42_RS10265 0.33 +0.1 2,198,022 - RR42_RS10265 0.33 -0.8 2,198,190 - RR42_RS10265 0.41 -0.5 2,198,269 + RR42_RS10265 0.45 -0.5 2,198,270 - RR42_RS10265 0.45 -0.6 2,198,283 + RR42_RS10265 0.46 +1.7 2,198,284 - RR42_RS10265 0.46 -0.4
Or see this region's nucleotide sequence