Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS10025

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS10015 and RR42_RS10020 are separated by 58 nucleotidesRR42_RS10020 and RR42_RS10025 are separated by 93 nucleotidesRR42_RS10025 and RR42_RS10030 are separated by 324 nucleotides RR42_RS10015: RR42_RS10015 - 4-methylmuconolactone transporter, at 2,139,503 to 2,140,810 _RS10015 RR42_RS10020: RR42_RS10020 - 4-methylmuconolactone methylisomerase, at 2,140,869 to 2,141,207 _RS10020 RR42_RS10025: RR42_RS10025 - muconolactone delta-isomerase, at 2,141,301 to 2,141,576 _RS10025 RR42_RS10030: RR42_RS10030 - 3-hydroxyisobutyrate dehydrogenase, at 2,141,901 to 2,142,290 _RS10030 Position (kb) 2141 2142Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2140.409 kb on + strand, within RR42_RS10015at 2140.409 kb on + strand, within RR42_RS10015at 2140.410 kb on - strand, within RR42_RS10015at 2140.537 kb on + strand, within RR42_RS10015at 2140.537 kb on + strand, within RR42_RS10015at 2140.538 kb on - strand, within RR42_RS10015at 2140.662 kb on - strand, within RR42_RS10015at 2140.662 kb on - strand, within RR42_RS10015at 2140.662 kb on - strand, within RR42_RS10015at 2140.839 kb on + strandat 2140.839 kb on + strandat 2140.839 kb on + strandat 2140.839 kb on + strandat 2140.839 kb on + strandat 2140.839 kb on + strandat 2140.840 kb on - strandat 2140.884 kb on + strandat 2140.983 kb on + strand, within RR42_RS10020at 2140.983 kb on + strand, within RR42_RS10020at 2141.091 kb on + strand, within RR42_RS10020at 2141.100 kb on + strand, within RR42_RS10020at 2141.101 kb on - strand, within RR42_RS10020at 2141.101 kb on - strand, within RR42_RS10020at 2141.101 kb on - strand, within RR42_RS10020at 2141.307 kb on + strandat 2141.428 kb on + strand, within RR42_RS10025at 2141.428 kb on + strand, within RR42_RS10025at 2141.428 kb on + strand, within RR42_RS10025at 2141.428 kb on + strand, within RR42_RS10025at 2141.429 kb on - strand, within RR42_RS10025at 2141.429 kb on - strand, within RR42_RS10025at 2141.449 kb on - strand, within RR42_RS10025at 2141.526 kb on + strand, within RR42_RS10025at 2141.526 kb on + strand, within RR42_RS10025at 2141.527 kb on - strand, within RR42_RS10025at 2141.527 kb on - strand, within RR42_RS10025at 2141.528 kb on + strand, within RR42_RS10025at 2141.528 kb on + strand, within RR42_RS10025at 2141.528 kb on + strand, within RR42_RS10025at 2141.528 kb on + strand, within RR42_RS10025at 2141.528 kb on + strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.529 kb on - strand, within RR42_RS10025at 2141.668 kb on + strandat 2141.977 kb on + strand, within RR42_RS10030at 2141.977 kb on + strand, within RR42_RS10030at 2141.977 kb on + strand, within RR42_RS10030at 2141.978 kb on - strand, within RR42_RS10030at 2141.978 kb on - strand, within RR42_RS10030at 2142.001 kb on + strand, within RR42_RS10030at 2142.058 kb on + strand, within RR42_RS10030at 2142.058 kb on + strand, within RR42_RS10030at 2142.058 kb on + strand, within RR42_RS10030at 2142.058 kb on + strand, within RR42_RS10030at 2142.058 kb on + strand, within RR42_RS10030at 2142.059 kb on - strand, within RR42_RS10030at 2142.193 kb on + strand, within RR42_RS10030at 2142.193 kb on + strand, within RR42_RS10030at 2142.194 kb on - strand, within RR42_RS10030at 2142.194 kb on - strand, within RR42_RS10030at 2142.226 kb on + strand, within RR42_RS10030at 2142.226 kb on + strand, within RR42_RS10030at 2142.227 kb on - strand, within RR42_RS10030at 2142.252 kb on + strandat 2142.305 kb on + strandat 2142.306 kb on - strandat 2142.306 kb on - strandat 2142.306 kb on - strandat 2142.548 kb on + strandat 2142.549 kb on - strandat 2142.552 kb on - strandat 2142.552 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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2,140,409 + RR42_RS10015 0.69 +0.2
2,140,409 + RR42_RS10015 0.69 -0.5
2,140,410 - RR42_RS10015 0.69 -0.8
2,140,537 + RR42_RS10015 0.79 -2.9
2,140,537 + RR42_RS10015 0.79 +0.6
2,140,538 - RR42_RS10015 0.79 +1.6
2,140,662 - RR42_RS10015 0.89 -0.0
2,140,662 - RR42_RS10015 0.89 -0.1
2,140,662 - RR42_RS10015 0.89 -0.1
2,140,839 + -1.7
2,140,839 + -0.7
2,140,839 + -1.6
2,140,839 + +0.5
2,140,839 + -0.7
2,140,839 + +0.3
2,140,840 - -2.7
2,140,884 + +0.2
2,140,983 + RR42_RS10020 0.34 +1.5
2,140,983 + RR42_RS10020 0.34 -0.6
2,141,091 + RR42_RS10020 0.65 -0.1
2,141,100 + RR42_RS10020 0.68 -2.2
2,141,101 - RR42_RS10020 0.68 +0.4
2,141,101 - RR42_RS10020 0.68 +1.2
2,141,101 - RR42_RS10020 0.68 +0.4
2,141,307 + +0.2
2,141,428 + RR42_RS10025 0.46 +0.3
2,141,428 + RR42_RS10025 0.46 +1.0
2,141,428 + RR42_RS10025 0.46 -0.9
2,141,428 + RR42_RS10025 0.46 +5.6
2,141,429 - RR42_RS10025 0.46 +0.4
2,141,429 - RR42_RS10025 0.46 +1.2
2,141,449 - RR42_RS10025 0.54 -1.4
2,141,526 + RR42_RS10025 0.82 +0.5
2,141,526 + RR42_RS10025 0.82 +0.7
2,141,527 - RR42_RS10025 0.82 +0.8
2,141,527 - RR42_RS10025 0.82 -1.3
2,141,528 + RR42_RS10025 0.82 +1.0
2,141,528 + RR42_RS10025 0.82 -2.4
2,141,528 + RR42_RS10025 0.82 -0.4
2,141,528 + RR42_RS10025 0.82 -0.9
2,141,528 + RR42_RS10025 0.82 -0.1
2,141,529 - RR42_RS10025 0.83 -0.0
2,141,529 - RR42_RS10025 0.83 -0.7
2,141,529 - RR42_RS10025 0.83 +0.6
2,141,529 - RR42_RS10025 0.83 -0.5
2,141,529 - RR42_RS10025 0.83 +1.0
2,141,529 - RR42_RS10025 0.83 -0.5
2,141,668 + +2.5
2,141,977 + RR42_RS10030 0.19 -1.4
2,141,977 + RR42_RS10030 0.19 -1.5
2,141,977 + RR42_RS10030 0.19 -0.0
2,141,978 - RR42_RS10030 0.20 -0.1
2,141,978 - RR42_RS10030 0.20 -1.4
2,142,001 + RR42_RS10030 0.26 -0.9
2,142,058 + RR42_RS10030 0.40 +0.6
2,142,058 + RR42_RS10030 0.40 -0.7
2,142,058 + RR42_RS10030 0.40 -3.4
2,142,058 + RR42_RS10030 0.40 -0.5
2,142,058 + RR42_RS10030 0.40 -1.7
2,142,059 - RR42_RS10030 0.41 -1.3
2,142,193 + RR42_RS10030 0.75 +0.8
2,142,193 + RR42_RS10030 0.75 -0.4
2,142,194 - RR42_RS10030 0.75 -1.7
2,142,194 - RR42_RS10030 0.75 -0.6
2,142,226 + RR42_RS10030 0.83 +1.1
2,142,226 + RR42_RS10030 0.83 +0.1
2,142,227 - RR42_RS10030 0.84 -1.9
2,142,252 + -0.0
2,142,305 + -0.3
2,142,306 - -1.9
2,142,306 - -0.1
2,142,306 - -2.6
2,142,548 + -2.2
2,142,549 - +0.0
2,142,552 - -0.8
2,142,552 - -1.1

Or see this region's nucleotide sequence