Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09990 and RR42_RS09995 are separated by 539 nucleotides RR42_RS09995 and RR42_RS10000 are separated by 107 nucleotides
RR42_RS09990: RR42_RS09990 - DeoR family transcriptional regulator, at 2,133,913 to 2,135,271
_RS09990
RR42_RS09995: RR42_RS09995 - MBL fold metallo-hydrolase, at 2,135,811 to 2,136,701
_RS09995
RR42_RS10000: RR42_RS10000 - LysR family transcriptional regulator, at 2,136,809 to 2,137,723
_RS10000
Position (kb)
2135
2136
2137 Strain fitness (log2 ratio)
-1
0
1
2 at 2134.859 kb on - strand, within RR42_RS09990 at 2134.864 kb on - strand, within RR42_RS09990 at 2135.024 kb on + strand, within RR42_RS09990 at 2135.068 kb on - strand, within RR42_RS09990 at 2135.097 kb on + strand, within RR42_RS09990 at 2135.113 kb on + strand, within RR42_RS09990 at 2135.218 kb on - strand at 2135.299 kb on + strand at 2135.331 kb on + strand at 2135.331 kb on + strand at 2135.332 kb on - strand at 2135.332 kb on - strand at 2135.336 kb on - strand at 2135.336 kb on - strand at 2135.358 kb on + strand at 2135.359 kb on - strand at 2135.494 kb on - strand at 2135.669 kb on + strand at 2135.786 kb on - strand at 2135.786 kb on - strand at 2135.802 kb on + strand at 2135.802 kb on + strand at 2135.803 kb on - strand at 2136.021 kb on - strand, within RR42_RS09995 at 2136.240 kb on + strand, within RR42_RS09995 at 2136.243 kb on + strand, within RR42_RS09995 at 2136.243 kb on + strand, within RR42_RS09995 at 2136.409 kb on + strand, within RR42_RS09995 at 2136.409 kb on + strand, within RR42_RS09995 at 2136.428 kb on + strand, within RR42_RS09995 at 2136.651 kb on + strand at 2136.768 kb on + strand at 2136.808 kb on + strand at 2136.808 kb on + strand at 2136.808 kb on + strand at 2136.809 kb on - strand at 2137.011 kb on - strand, within RR42_RS10000 at 2137.134 kb on - strand, within RR42_RS10000 at 2137.418 kb on + strand, within RR42_RS10000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,134,859 - RR42_RS09990 0.70 -0.1 2,134,864 - RR42_RS09990 0.70 +0.2 2,135,024 + RR42_RS09990 0.82 +0.6 2,135,068 - RR42_RS09990 0.85 +0.5 2,135,097 + RR42_RS09990 0.87 +0.6 2,135,113 + RR42_RS09990 0.88 +0.4 2,135,218 - +1.4 2,135,299 + +0.2 2,135,331 + +0.5 2,135,331 + +1.6 2,135,332 - -0.1 2,135,332 - -1.6 2,135,336 - -0.6 2,135,336 - -1.3 2,135,358 + +0.1 2,135,359 - -1.3 2,135,494 - +0.5 2,135,669 + -0.5 2,135,786 - +0.8 2,135,786 - -0.7 2,135,802 + +0.5 2,135,802 + +0.8 2,135,803 - +0.5 2,136,021 - RR42_RS09995 0.24 -1.7 2,136,240 + RR42_RS09995 0.48 +0.6 2,136,243 + RR42_RS09995 0.48 +1.8 2,136,243 + RR42_RS09995 0.48 +2.5 2,136,409 + RR42_RS09995 0.67 +0.6 2,136,409 + RR42_RS09995 0.67 -1.2 2,136,428 + RR42_RS09995 0.69 +0.8 2,136,651 + +0.2 2,136,768 + -0.2 2,136,808 + +1.7 2,136,808 + +1.3 2,136,808 + -0.7 2,136,809 - -1.7 2,137,011 - RR42_RS10000 0.22 -0.4 2,137,134 - RR42_RS10000 0.36 +0.4 2,137,418 + RR42_RS10000 0.67 -1.5
Or see this region's nucleotide sequence