Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09905 and RR42_RS09910 are separated by 7 nucleotides RR42_RS09910 and RR42_RS09915 are separated by 46 nucleotides
RR42_RS09905: RR42_RS09905 - fumarylacetoacetase, at 2,113,460 to 2,114,773
_RS09905
RR42_RS09910: RR42_RS09910 - cyclase, at 2,114,781 to 2,115,584
_RS09910
RR42_RS09915: RR42_RS09915 - MFS transporter, at 2,115,631 to 2,116,896
_RS09915
Position (kb)
2114
2115
2116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2113.888 kb on - strand, within RR42_RS09905 at 2113.888 kb on - strand, within RR42_RS09905 at 2114.075 kb on + strand, within RR42_RS09905 at 2114.075 kb on + strand, within RR42_RS09905 at 2114.075 kb on + strand, within RR42_RS09905 at 2114.110 kb on + strand, within RR42_RS09905 at 2114.157 kb on + strand, within RR42_RS09905 at 2114.230 kb on - strand, within RR42_RS09905 at 2114.359 kb on - strand, within RR42_RS09905 at 2114.371 kb on - strand, within RR42_RS09905 at 2114.613 kb on - strand, within RR42_RS09905 at 2114.631 kb on - strand, within RR42_RS09905 at 2114.903 kb on + strand, within RR42_RS09910 at 2114.903 kb on + strand, within RR42_RS09910 at 2114.903 kb on + strand, within RR42_RS09910 at 2114.904 kb on - strand, within RR42_RS09910 at 2114.926 kb on + strand, within RR42_RS09910 at 2114.962 kb on - strand, within RR42_RS09910 at 2115.052 kb on - strand, within RR42_RS09910 at 2115.070 kb on + strand, within RR42_RS09910 at 2115.070 kb on + strand, within RR42_RS09910 at 2115.112 kb on + strand, within RR42_RS09910 at 2115.113 kb on - strand, within RR42_RS09910 at 2115.113 kb on - strand, within RR42_RS09910 at 2115.128 kb on - strand, within RR42_RS09910 at 2115.128 kb on - strand, within RR42_RS09910 at 2115.229 kb on + strand, within RR42_RS09910 at 2115.358 kb on + strand, within RR42_RS09910 at 2115.358 kb on + strand, within RR42_RS09910 at 2115.359 kb on - strand, within RR42_RS09910 at 2115.389 kb on + strand, within RR42_RS09910 at 2115.390 kb on - strand, within RR42_RS09910 at 2115.611 kb on + strand at 2115.612 kb on - strand at 2115.612 kb on - strand at 2115.899 kb on + strand, within RR42_RS09915 at 2115.900 kb on - strand, within RR42_RS09915 at 2115.984 kb on + strand, within RR42_RS09915 at 2115.987 kb on - strand, within RR42_RS09915 at 2115.987 kb on - strand, within RR42_RS09915 at 2116.077 kb on - strand, within RR42_RS09915 at 2116.077 kb on - strand, within RR42_RS09915 at 2116.230 kb on + strand, within RR42_RS09915 at 2116.306 kb on - strand, within RR42_RS09915 at 2116.355 kb on + strand, within RR42_RS09915 at 2116.355 kb on + strand, within RR42_RS09915 at 2116.355 kb on + strand, within RR42_RS09915 at 2116.356 kb on - strand, within RR42_RS09915 at 2116.412 kb on + strand, within RR42_RS09915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,113,888 - RR42_RS09905 0.33 +2.5 2,113,888 - RR42_RS09905 0.33 +0.1 2,114,075 + RR42_RS09905 0.47 -0.4 2,114,075 + RR42_RS09905 0.47 -2.2 2,114,075 + RR42_RS09905 0.47 +0.3 2,114,110 + RR42_RS09905 0.49 -1.4 2,114,157 + RR42_RS09905 0.53 +1.0 2,114,230 - RR42_RS09905 0.59 -2.2 2,114,359 - RR42_RS09905 0.68 +1.2 2,114,371 - RR42_RS09905 0.69 -0.3 2,114,613 - RR42_RS09905 0.88 -1.5 2,114,631 - RR42_RS09905 0.89 -1.2 2,114,903 + RR42_RS09910 0.15 +0.8 2,114,903 + RR42_RS09910 0.15 -0.8 2,114,903 + RR42_RS09910 0.15 +0.2 2,114,904 - RR42_RS09910 0.15 +0.5 2,114,926 + RR42_RS09910 0.18 +0.4 2,114,962 - RR42_RS09910 0.23 -0.2 2,115,052 - RR42_RS09910 0.34 -1.4 2,115,070 + RR42_RS09910 0.36 +0.2 2,115,070 + RR42_RS09910 0.36 -0.4 2,115,112 + RR42_RS09910 0.41 -1.2 2,115,113 - RR42_RS09910 0.41 -1.1 2,115,113 - RR42_RS09910 0.41 +1.3 2,115,128 - RR42_RS09910 0.43 +0.4 2,115,128 - RR42_RS09910 0.43 -1.3 2,115,229 + RR42_RS09910 0.56 -2.7 2,115,358 + RR42_RS09910 0.72 -0.4 2,115,358 + RR42_RS09910 0.72 -3.5 2,115,359 - RR42_RS09910 0.72 +0.1 2,115,389 + RR42_RS09910 0.76 +0.5 2,115,390 - RR42_RS09910 0.76 -0.0 2,115,611 + -1.2 2,115,612 - +2.4 2,115,612 - -0.7 2,115,899 + RR42_RS09915 0.21 -0.3 2,115,900 - RR42_RS09915 0.21 +0.5 2,115,984 + RR42_RS09915 0.28 +0.2 2,115,987 - RR42_RS09915 0.28 +0.5 2,115,987 - RR42_RS09915 0.28 -0.1 2,116,077 - RR42_RS09915 0.35 +0.1 2,116,077 - RR42_RS09915 0.35 -0.4 2,116,230 + RR42_RS09915 0.47 +1.8 2,116,306 - RR42_RS09915 0.53 -0.2 2,116,355 + RR42_RS09915 0.57 +0.6 2,116,355 + RR42_RS09915 0.57 -0.9 2,116,355 + RR42_RS09915 0.57 -1.0 2,116,356 - RR42_RS09915 0.57 +0.9 2,116,412 + RR42_RS09915 0.62 -0.7
Or see this region's nucleotide sequence