Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09740 and RR42_RS09745 are separated by 269 nucleotides RR42_RS09745 and RR42_RS09750 are separated by 191 nucleotides
RR42_RS09740: RR42_RS09740 - hemolysin secretion protein D, at 2,074,356 to 2,075,342
_RS09740
RR42_RS09745: RR42_RS09745 - alcohol dehydrogenase, at 2,075,612 to 2,076,619
_RS09745
RR42_RS09750: RR42_RS09750 - universal stress protein UspA, at 2,076,811 to 2,077,641
_RS09750
Position (kb)
2075
2076
2077 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.686 kb on - strand, within RR42_RS09740 at 2074.686 kb on - strand, within RR42_RS09740 at 2074.781 kb on + strand, within RR42_RS09740 at 2074.957 kb on + strand, within RR42_RS09740 at 2075.095 kb on - strand, within RR42_RS09740 at 2075.095 kb on - strand, within RR42_RS09740 at 2075.183 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.310 kb on + strand at 2075.310 kb on + strand at 2075.311 kb on - strand at 2075.319 kb on - strand at 2075.479 kb on - strand at 2075.479 kb on - strand at 2075.554 kb on + strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.557 kb on + strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.587 kb on + strand at 2075.587 kb on + strand at 2075.588 kb on - strand at 2075.827 kb on + strand, within RR42_RS09745 at 2076.097 kb on - strand, within RR42_RS09745 at 2076.097 kb on - strand, within RR42_RS09745 at 2076.198 kb on + strand, within RR42_RS09745 at 2076.198 kb on + strand, within RR42_RS09745 at 2076.212 kb on - strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.243 kb on + strand, within RR42_RS09745 at 2076.252 kb on + strand, within RR42_RS09745 at 2076.268 kb on + strand, within RR42_RS09745 at 2076.269 kb on - strand, within RR42_RS09745 at 2076.322 kb on - strand, within RR42_RS09745 at 2076.322 kb on - strand, within RR42_RS09745 at 2076.389 kb on + strand, within RR42_RS09745 at 2076.648 kb on + strand at 2076.648 kb on + strand at 2076.648 kb on + strand at 2076.649 kb on - strand at 2076.725 kb on + strand at 2076.767 kb on + strand at 2076.767 kb on + strand at 2076.768 kb on - strand at 2076.817 kb on + strand at 2076.817 kb on + strand at 2076.817 kb on + strand at 2076.818 kb on - strand at 2076.818 kb on - strand at 2076.933 kb on + strand, within RR42_RS09750 at 2076.956 kb on - strand, within RR42_RS09750 at 2076.956 kb on - strand, within RR42_RS09750 at 2076.979 kb on + strand, within RR42_RS09750 at 2076.979 kb on + strand, within RR42_RS09750 at 2076.979 kb on + strand, within RR42_RS09750 at 2076.979 kb on + strand, within RR42_RS09750 at 2076.979 kb on + strand, within RR42_RS09750 at 2076.980 kb on - strand, within RR42_RS09750 at 2077.153 kb on + strand, within RR42_RS09750 at 2077.153 kb on + strand, within RR42_RS09750 at 2077.153 kb on + strand, within RR42_RS09750 at 2077.154 kb on - strand, within RR42_RS09750 at 2077.154 kb on - strand, within RR42_RS09750 at 2077.154 kb on - strand, within RR42_RS09750 at 2077.233 kb on + strand, within RR42_RS09750 at 2077.234 kb on - strand, within RR42_RS09750 at 2077.550 kb on - strand, within RR42_RS09750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,074,681 + RR42_RS09740 0.33 -0.3 2,074,681 + RR42_RS09740 0.33 -1.1 2,074,681 + RR42_RS09740 0.33 +0.6 2,074,681 + RR42_RS09740 0.33 -0.7 2,074,681 + RR42_RS09740 0.33 +0.1 2,074,681 + RR42_RS09740 0.33 -1.4 2,074,681 + RR42_RS09740 0.33 +1.4 2,074,682 - RR42_RS09740 0.33 +0.6 2,074,682 - RR42_RS09740 0.33 -0.8 2,074,682 - RR42_RS09740 0.33 +0.6 2,074,682 - RR42_RS09740 0.33 +1.5 2,074,682 - RR42_RS09740 0.33 -1.4 2,074,686 - RR42_RS09740 0.33 -0.4 2,074,686 - RR42_RS09740 0.33 -0.1 2,074,781 + RR42_RS09740 0.43 +0.4 2,074,957 + RR42_RS09740 0.61 -2.5 2,075,095 - RR42_RS09740 0.75 -2.2 2,075,095 - RR42_RS09740 0.75 +0.8 2,075,183 - RR42_RS09740 0.84 +0.0 2,075,240 - RR42_RS09740 0.90 +0.0 2,075,240 - RR42_RS09740 0.90 -2.2 2,075,240 - RR42_RS09740 0.90 +0.4 2,075,240 - RR42_RS09740 0.90 +1.4 2,075,310 + -1.3 2,075,310 + +1.3 2,075,311 - -0.9 2,075,319 - -0.5 2,075,479 - -2.2 2,075,479 - +0.8 2,075,554 + -1.1 2,075,555 - -0.5 2,075,555 - +1.0 2,075,555 - -1.4 2,075,555 - -1.0 2,075,557 + -1.2 2,075,558 - +1.0 2,075,558 - -3.3 2,075,558 - -0.3 2,075,558 - +0.2 2,075,558 - -0.2 2,075,587 + +0.2 2,075,587 + -0.2 2,075,588 - -3.4 2,075,827 + RR42_RS09745 0.21 -0.4 2,076,097 - RR42_RS09745 0.48 -1.7 2,076,097 - RR42_RS09745 0.48 -1.7 2,076,198 + RR42_RS09745 0.58 -2.1 2,076,198 + RR42_RS09745 0.58 +1.5 2,076,212 - RR42_RS09745 0.60 +0.6 2,076,222 + RR42_RS09745 0.61 +0.1 2,076,222 + RR42_RS09745 0.61 -0.2 2,076,222 + RR42_RS09745 0.61 -0.7 2,076,243 + RR42_RS09745 0.63 -2.7 2,076,252 + RR42_RS09745 0.63 +0.1 2,076,268 + RR42_RS09745 0.65 -1.2 2,076,269 - RR42_RS09745 0.65 -0.6 2,076,322 - RR42_RS09745 0.70 -0.5 2,076,322 - RR42_RS09745 0.70 +0.6 2,076,389 + RR42_RS09745 0.77 +0.9 2,076,648 + -0.8 2,076,648 + +0.1 2,076,648 + +1.0 2,076,649 - +0.1 2,076,725 + +0.4 2,076,767 + +0.2 2,076,767 + -0.6 2,076,768 - +0.1 2,076,817 + +0.6 2,076,817 + -0.5 2,076,817 + +0.3 2,076,818 - +0.2 2,076,818 - +3.5 2,076,933 + RR42_RS09750 0.15 -0.1 2,076,956 - RR42_RS09750 0.17 +1.7 2,076,956 - RR42_RS09750 0.17 -0.4 2,076,979 + RR42_RS09750 0.20 -1.7 2,076,979 + RR42_RS09750 0.20 -0.0 2,076,979 + RR42_RS09750 0.20 -0.6 2,076,979 + RR42_RS09750 0.20 +1.6 2,076,979 + RR42_RS09750 0.20 +0.6 2,076,980 - RR42_RS09750 0.20 -1.5 2,077,153 + RR42_RS09750 0.41 -1.8 2,077,153 + RR42_RS09750 0.41 +0.4 2,077,153 + RR42_RS09750 0.41 -0.1 2,077,154 - RR42_RS09750 0.41 -1.7 2,077,154 - RR42_RS09750 0.41 +1.8 2,077,154 - RR42_RS09750 0.41 -0.0 2,077,233 + RR42_RS09750 0.51 +1.2 2,077,234 - RR42_RS09750 0.51 +1.9 2,077,550 - RR42_RS09750 0.89 -0.0
Or see this region's nucleotide sequence