Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09500 and RR42_RS09505 are separated by 205 nucleotides RR42_RS09505 and RR42_RS09510 are separated by 282 nucleotides
RR42_RS09500: RR42_RS09500 - dehydrogenase, at 2,014,032 to 2,015,054
_RS09500
RR42_RS09505: RR42_RS09505 - fatty acid hydroxylase, at 2,015,260 to 2,016,180
_RS09505
RR42_RS09510: RR42_RS09510 - hypothetical protein, at 2,016,463 to 2,017,416
_RS09510
Position (kb)
2015
2016
2017 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2014.766 kb on + strand, within RR42_RS09500 at 2014.766 kb on + strand, within RR42_RS09500 at 2014.766 kb on + strand, within RR42_RS09500 at 2014.767 kb on - strand, within RR42_RS09500 at 2014.767 kb on - strand, within RR42_RS09500 at 2014.767 kb on - strand, within RR42_RS09500 at 2014.769 kb on - strand, within RR42_RS09500 at 2014.769 kb on - strand, within RR42_RS09500 at 2014.769 kb on - strand, within RR42_RS09500 at 2014.903 kb on + strand, within RR42_RS09500 at 2015.011 kb on + strand at 2015.011 kb on + strand at 2015.012 kb on - strand at 2015.122 kb on + strand at 2015.122 kb on + strand at 2015.454 kb on + strand, within RR42_RS09505 at 2015.454 kb on + strand, within RR42_RS09505 at 2015.510 kb on + strand, within RR42_RS09505 at 2015.510 kb on + strand, within RR42_RS09505 at 2015.510 kb on + strand, within RR42_RS09505 at 2015.510 kb on + strand, within RR42_RS09505 at 2015.511 kb on - strand, within RR42_RS09505 at 2015.552 kb on + strand, within RR42_RS09505 at 2015.568 kb on - strand, within RR42_RS09505 at 2015.621 kb on + strand, within RR42_RS09505 at 2015.622 kb on - strand, within RR42_RS09505 at 2015.622 kb on - strand, within RR42_RS09505 at 2015.672 kb on + strand, within RR42_RS09505 at 2015.786 kb on + strand, within RR42_RS09505 at 2015.787 kb on - strand, within RR42_RS09505 at 2015.873 kb on + strand, within RR42_RS09505 at 2015.880 kb on - strand, within RR42_RS09505 at 2015.882 kb on + strand, within RR42_RS09505 at 2015.883 kb on - strand, within RR42_RS09505 at 2015.943 kb on + strand, within RR42_RS09505 at 2015.943 kb on + strand, within RR42_RS09505 at 2016.152 kb on + strand at 2016.152 kb on + strand at 2016.153 kb on - strand at 2016.255 kb on + strand at 2016.257 kb on - strand at 2016.290 kb on + strand at 2016.290 kb on + strand at 2016.291 kb on - strand at 2016.291 kb on - strand at 2016.291 kb on - strand at 2016.343 kb on + strand at 2016.344 kb on - strand at 2016.390 kb on + strand at 2016.391 kb on - strand at 2016.391 kb on - strand at 2016.392 kb on + strand at 2016.393 kb on - strand at 2016.574 kb on + strand, within RR42_RS09510 at 2016.574 kb on + strand, within RR42_RS09510 at 2016.575 kb on - strand, within RR42_RS09510 at 2016.575 kb on - strand at 2016.741 kb on - strand, within RR42_RS09510 at 2016.863 kb on - strand, within RR42_RS09510 at 2016.863 kb on - strand, within RR42_RS09510 at 2017.171 kb on + strand, within RR42_RS09510 at 2017.172 kb on - strand, within RR42_RS09510 at 2017.172 kb on - strand, within RR42_RS09510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,014,766 + RR42_RS09500 0.72 +1.0 2,014,766 + RR42_RS09500 0.72 -1.9 2,014,766 + RR42_RS09500 0.72 +0.7 2,014,767 - RR42_RS09500 0.72 +0.1 2,014,767 - RR42_RS09500 0.72 +1.1 2,014,767 - RR42_RS09500 0.72 +0.1 2,014,769 - RR42_RS09500 0.72 +1.5 2,014,769 - RR42_RS09500 0.72 -0.5 2,014,769 - RR42_RS09500 0.72 +0.8 2,014,903 + RR42_RS09500 0.85 -0.9 2,015,011 + -0.3 2,015,011 + -1.2 2,015,012 - +0.1 2,015,122 + +0.1 2,015,122 + +0.2 2,015,454 + RR42_RS09505 0.21 +1.3 2,015,454 + RR42_RS09505 0.21 -0.0 2,015,510 + RR42_RS09505 0.27 -1.3 2,015,510 + RR42_RS09505 0.27 +1.0 2,015,510 + RR42_RS09505 0.27 -0.2 2,015,510 + RR42_RS09505 0.27 +1.0 2,015,511 - RR42_RS09505 0.27 -0.8 2,015,552 + RR42_RS09505 0.32 -0.9 2,015,568 - RR42_RS09505 0.33 -1.5 2,015,621 + RR42_RS09505 0.39 -0.2 2,015,622 - RR42_RS09505 0.39 -0.2 2,015,622 - RR42_RS09505 0.39 -0.7 2,015,672 + RR42_RS09505 0.45 +2.2 2,015,786 + RR42_RS09505 0.57 +2.3 2,015,787 - RR42_RS09505 0.57 -0.2 2,015,873 + RR42_RS09505 0.67 -0.6 2,015,880 - RR42_RS09505 0.67 +1.9 2,015,882 + RR42_RS09505 0.68 -1.3 2,015,883 - RR42_RS09505 0.68 +1.7 2,015,943 + RR42_RS09505 0.74 -0.9 2,015,943 + RR42_RS09505 0.74 +0.9 2,016,152 + +1.1 2,016,152 + -1.8 2,016,153 - +0.2 2,016,255 + +0.3 2,016,257 - +0.3 2,016,290 + -0.5 2,016,290 + -0.2 2,016,291 - -0.3 2,016,291 - +0.4 2,016,291 - -0.2 2,016,343 + -0.3 2,016,344 - +1.4 2,016,390 + +1.5 2,016,391 - +0.5 2,016,391 - +0.8 2,016,392 + +0.2 2,016,393 - -1.1 2,016,574 + RR42_RS09510 0.12 +2.2 2,016,574 + RR42_RS09510 0.12 +0.7 2,016,575 - RR42_RS09510 0.12 +2.5 2,016,575 - -1.3 2,016,741 - RR42_RS09510 0.29 +0.1 2,016,863 - RR42_RS09510 0.42 -0.5 2,016,863 - RR42_RS09510 0.42 +0.1 2,017,171 + RR42_RS09510 0.74 +1.2 2,017,172 - RR42_RS09510 0.74 +1.8 2,017,172 - RR42_RS09510 0.74 -0.7
Or see this region's nucleotide sequence