Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09465 and RR42_RS09470 are separated by 20 nucleotides RR42_RS09470 and RR42_RS09475 overlap by 4 nucleotides RR42_RS09475 and RR42_RS09480 are separated by 23 nucleotides
RR42_RS09465: RR42_RS09465 - bifunctional glyoxylate/hydroxypyruvate reductase B, at 2,008,115 to 2,009,092
_RS09465
RR42_RS09470: RR42_RS09470 - 2-dehydro-3-deoxygluconokinase, at 2,009,113 to 2,010,057
_RS09470
RR42_RS09475: RR42_RS09475 - glutamine ABC transporter ATP-binding protein, at 2,010,054 to 2,010,815
_RS09475
RR42_RS09480: RR42_RS09480 - amino acid ABC transporter ATP-binding protein, at 2,010,839 to 2,011,573
_RS09480
Position (kb)
2009
2010
2011 Strain fitness (log2 ratio)
-2
-1
0
1 at 2008.572 kb on + strand, within RR42_RS09465 at 2008.572 kb on + strand, within RR42_RS09465 at 2008.572 kb on + strand, within RR42_RS09465 at 2008.573 kb on - strand, within RR42_RS09465 at 2008.573 kb on - strand, within RR42_RS09465 at 2008.573 kb on - strand, within RR42_RS09465 at 2008.694 kb on - strand, within RR42_RS09465 at 2008.800 kb on - strand, within RR42_RS09465 at 2008.811 kb on - strand, within RR42_RS09465 at 2008.823 kb on - strand, within RR42_RS09465 at 2008.823 kb on - strand, within RR42_RS09465 at 2008.823 kb on - strand, within RR42_RS09465 at 2008.854 kb on + strand, within RR42_RS09465 at 2008.854 kb on + strand, within RR42_RS09465 at 2008.854 kb on + strand, within RR42_RS09465 at 2008.854 kb on + strand, within RR42_RS09465 at 2008.855 kb on - strand, within RR42_RS09465 at 2008.855 kb on - strand, within RR42_RS09465 at 2009.078 kb on + strand at 2009.079 kb on - strand at 2009.349 kb on - strand, within RR42_RS09470 at 2009.735 kb on + strand, within RR42_RS09470 at 2010.109 kb on - strand at 2010.174 kb on + strand, within RR42_RS09475 at 2010.175 kb on - strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.259 kb on + strand, within RR42_RS09475 at 2010.582 kb on - strand, within RR42_RS09475 at 2010.627 kb on + strand, within RR42_RS09475 at 2010.627 kb on + strand, within RR42_RS09475 at 2010.781 kb on + strand at 2010.782 kb on - strand at 2010.955 kb on + strand, within RR42_RS09480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,008,572 + RR42_RS09465 0.47 +0.1 2,008,572 + RR42_RS09465 0.47 +1.2 2,008,572 + RR42_RS09465 0.47 -0.7 2,008,573 - RR42_RS09465 0.47 +0.3 2,008,573 - RR42_RS09465 0.47 +1.1 2,008,573 - RR42_RS09465 0.47 -0.6 2,008,694 - RR42_RS09465 0.59 -1.0 2,008,800 - RR42_RS09465 0.70 -0.8 2,008,811 - RR42_RS09465 0.71 -2.2 2,008,823 - RR42_RS09465 0.72 -2.3 2,008,823 - RR42_RS09465 0.72 -0.6 2,008,823 - RR42_RS09465 0.72 +0.1 2,008,854 + RR42_RS09465 0.76 -0.1 2,008,854 + RR42_RS09465 0.76 +0.0 2,008,854 + RR42_RS09465 0.76 -0.6 2,008,854 + RR42_RS09465 0.76 +0.7 2,008,855 - RR42_RS09465 0.76 +0.5 2,008,855 - RR42_RS09465 0.76 +1.1 2,009,078 + +0.4 2,009,079 - -0.4 2,009,349 - RR42_RS09470 0.25 -0.6 2,009,735 + RR42_RS09470 0.66 -2.2 2,010,109 - +0.1 2,010,174 + RR42_RS09475 0.16 -0.8 2,010,175 - RR42_RS09475 0.16 +0.5 2,010,259 + RR42_RS09475 0.27 -0.3 2,010,259 + RR42_RS09475 0.27 -0.5 2,010,259 + RR42_RS09475 0.27 +1.2 2,010,259 + RR42_RS09475 0.27 +1.5 2,010,582 - RR42_RS09475 0.69 +0.3 2,010,627 + RR42_RS09475 0.75 -1.0 2,010,627 + RR42_RS09475 0.75 -0.3 2,010,781 + -0.4 2,010,782 - -0.1 2,010,955 + RR42_RS09480 0.16 +0.0
Or see this region's nucleotide sequence