Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09210 and RR42_RS09215 are separated by 4 nucleotides RR42_RS09215 and RR42_RS09220 are separated by 71 nucleotides RR42_RS09220 and RR42_RS09225 are separated by 115 nucleotides
RR42_RS09210: RR42_RS09210 - cbb3-type cytochrome C oxidase subunit III, at 1,947,587 to 1,948,195
_RS09210
RR42_RS09215: RR42_RS09215 - hypothetical protein, at 1,948,200 to 1,948,835
_RS09215
RR42_RS09220: RR42_RS09220 - hypothetical protein, at 1,948,907 to 1,949,428
_RS09220
RR42_RS09225: RR42_RS09225 - DSBA oxidoreductase, at 1,949,544 to 1,951,109
_RS09225
Position (kb) 1948
1949
1950 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1947.998 kb on + strand, within RR42_RS09210 at 1947.998 kb on + strand, within RR42_RS09210 at 1947.999 kb on - strand, within RR42_RS09210 at 1947.999 kb on - strand, within RR42_RS09210 at 1948.194 kb on - strand at 1948.450 kb on - strand, within RR42_RS09215 at 1948.550 kb on - strand, within RR42_RS09215 at 1948.555 kb on + strand, within RR42_RS09215 at 1948.599 kb on + strand, within RR42_RS09215 at 1948.599 kb on + strand, within RR42_RS09215 at 1948.600 kb on - strand, within RR42_RS09215 at 1948.806 kb on + strand at 1948.806 kb on + strand at 1949.181 kb on - strand, within RR42_RS09220 at 1949.268 kb on - strand, within RR42_RS09220 at 1949.268 kb on - strand, within RR42_RS09220 at 1949.271 kb on + strand, within RR42_RS09220 at 1949.271 kb on + strand, within RR42_RS09220 at 1949.271 kb on + strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.272 kb on - strand, within RR42_RS09220 at 1949.475 kb on - strand at 1949.490 kb on + strand at 1949.490 kb on + strand at 1949.491 kb on - strand at 1949.662 kb on - strand at 1950.194 kb on - strand, within RR42_RS09225 at 1950.244 kb on + strand, within RR42_RS09225 at 1950.351 kb on - strand, within RR42_RS09225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,947,998 + RR42_RS09210 0.67 +0.2 1,947,998 + RR42_RS09210 0.67 +1.0 1,947,999 - RR42_RS09210 0.68 +1.1 1,947,999 - RR42_RS09210 0.68 +0.7 1,948,194 - +0.2 1,948,450 - RR42_RS09215 0.39 +1.5 1,948,550 - RR42_RS09215 0.55 -0.3 1,948,555 + RR42_RS09215 0.56 +0.3 1,948,599 + RR42_RS09215 0.63 -2.3 1,948,599 + RR42_RS09215 0.63 -0.3 1,948,600 - RR42_RS09215 0.63 +0.1 1,948,806 + +0.2 1,948,806 + +0.4 1,949,181 - RR42_RS09220 0.52 -0.1 1,949,268 - RR42_RS09220 0.69 -2.2 1,949,268 - RR42_RS09220 0.69 -0.5 1,949,271 + RR42_RS09220 0.70 +0.9 1,949,271 + RR42_RS09220 0.70 -1.2 1,949,271 + RR42_RS09220 0.70 -0.3 1,949,272 - RR42_RS09220 0.70 -0.3 1,949,272 - RR42_RS09220 0.70 +0.8 1,949,272 - RR42_RS09220 0.70 -2.8 1,949,272 - RR42_RS09220 0.70 +2.3 1,949,272 - RR42_RS09220 0.70 -1.3 1,949,272 - RR42_RS09220 0.70 +1.7 1,949,272 - RR42_RS09220 0.70 +0.0 1,949,272 - RR42_RS09220 0.70 -0.8 1,949,475 - -0.7 1,949,490 + -1.3 1,949,490 + -0.1 1,949,491 - +0.7 1,949,662 - -1.9 1,950,194 - RR42_RS09225 0.42 +1.1 1,950,244 + RR42_RS09225 0.45 +0.2 1,950,351 - RR42_RS09225 0.52 +0.2
Or see this region's nucleotide sequence