Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09175 and RR42_RS09180 are separated by 302 nucleotides RR42_RS09180 and RR42_RS09185 are separated by 44 nucleotides RR42_RS09185 and RR42_RS09190 are separated by 96 nucleotides
RR42_RS09175: RR42_RS09175 - flavohemoprotein, at 1,939,333 to 1,940,544
_RS09175
RR42_RS09180: RR42_RS09180 - Rrf2 family transcriptional regulator, at 1,940,847 to 1,941,371
_RS09180
RR42_RS09185: RR42_RS09185 - alkylhydroperoxidase, at 1,941,416 to 1,941,853
_RS09185
RR42_RS09190: RR42_RS09190 - DNA-binding protein, at 1,941,950 to 1,943,401
_RS09190
Position (kb)
1940
1941
1942 Strain fitness (log2 ratio)
-1
0
1
2 at 1939.935 kb on - strand, within RR42_RS09175 at 1939.985 kb on + strand, within RR42_RS09175 at 1940.047 kb on - strand, within RR42_RS09175 at 1940.168 kb on + strand, within RR42_RS09175 at 1940.169 kb on - strand, within RR42_RS09175 at 1940.169 kb on - strand, within RR42_RS09175 at 1940.342 kb on + strand, within RR42_RS09175 at 1940.343 kb on - strand, within RR42_RS09175 at 1940.619 kb on - strand at 1940.739 kb on - strand at 1940.872 kb on - strand at 1940.893 kb on - strand at 1941.012 kb on - strand, within RR42_RS09180 at 1941.369 kb on + strand at 1941.370 kb on - strand at 1941.370 kb on - strand at 1941.706 kb on - strand, within RR42_RS09185 at 1941.831 kb on + strand at 1941.831 kb on + strand at 1941.912 kb on + strand at 1941.928 kb on + strand at 1941.929 kb on - strand at 1941.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,939,935 - RR42_RS09175 0.50 +0.7 1,939,985 + RR42_RS09175 0.54 +0.1 1,940,047 - RR42_RS09175 0.59 -1.3 1,940,168 + RR42_RS09175 0.69 -0.8 1,940,169 - RR42_RS09175 0.69 +0.1 1,940,169 - RR42_RS09175 0.69 +0.1 1,940,342 + RR42_RS09175 0.83 +0.0 1,940,343 - RR42_RS09175 0.83 -0.3 1,940,619 - -0.1 1,940,739 - +0.8 1,940,872 - +1.3 1,940,893 - -0.5 1,941,012 - RR42_RS09180 0.31 -1.0 1,941,369 + -0.3 1,941,370 - -0.6 1,941,370 - -1.3 1,941,706 - RR42_RS09185 0.66 +0.2 1,941,831 + +0.9 1,941,831 + +0.7 1,941,912 + +0.3 1,941,928 + +2.6 1,941,929 - -0.8 1,941,986 + -0.1
Or see this region's nucleotide sequence