Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09075 and RR42_RS09080 are separated by 49 nucleotides RR42_RS09080 and RR42_RS09085 overlap by 1 nucleotides RR42_RS09085 and RR42_RS09090 are separated by 223 nucleotides
RR42_RS09075: RR42_RS09075 - amino acid dehydrogenase, at 1,909,075 to 1,910,373
_RS09075
RR42_RS09080: RR42_RS09080 - C4-dicarboxylate ABC transporter permease, at 1,910,423 to 1,911,733
_RS09080
RR42_RS09085: RR42_RS09085 - C4-dicarboxylate ABC transporter permease, at 1,911,733 to 1,912,284
_RS09085
RR42_RS09090: RR42_RS09090 - C4-dicarboxylate ABC transporter substrate-binding protein, at 1,912,508 to 1,913,467
_RS09090
Position (kb)
1910
1911
1912 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1909.448 kb on + strand, within RR42_RS09075 at 1909.514 kb on + strand, within RR42_RS09075 at 1909.515 kb on - strand, within RR42_RS09075 at 1909.530 kb on - strand, within RR42_RS09075 at 1909.831 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075 at 1910.222 kb on + strand, within RR42_RS09075 at 1910.296 kb on + strand at 1910.297 kb on - strand at 1910.321 kb on + strand at 1910.322 kb on - strand at 1910.383 kb on + strand at 1910.384 kb on - strand at 1910.389 kb on - strand at 1910.424 kb on + strand at 1910.544 kb on + strand at 1910.544 kb on + strand at 1910.544 kb on + strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.549 kb on - strand at 1910.549 kb on - strand at 1910.639 kb on + strand, within RR42_RS09080 at 1910.651 kb on + strand, within RR42_RS09080 at 1910.652 kb on - strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.749 kb on - strand, within RR42_RS09080 at 1910.833 kb on + strand, within RR42_RS09080 at 1910.834 kb on - strand, within RR42_RS09080 at 1911.037 kb on + strand, within RR42_RS09080 at 1911.327 kb on + strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.557 kb on - strand, within RR42_RS09080 at 1912.094 kb on - strand, within RR42_RS09085 at 1912.152 kb on + strand, within RR42_RS09085 at 1912.392 kb on + strand at 1912.393 kb on - strand at 1912.413 kb on + strand at 1912.425 kb on + strand at 1912.461 kb on + strand at 1912.474 kb on - strand at 1912.519 kb on - strand at 1912.663 kb on - strand, within RR42_RS09090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,909,448 + RR42_RS09075 0.29 +0.8 1,909,514 + RR42_RS09075 0.34 +0.2 1,909,515 - RR42_RS09075 0.34 -0.2 1,909,530 - RR42_RS09075 0.35 -3.2 1,909,831 - RR42_RS09075 0.58 +0.7 1,910,034 - RR42_RS09075 0.74 -0.4 1,910,034 - RR42_RS09075 0.74 -1.0 1,910,222 + RR42_RS09075 0.88 -0.8 1,910,296 + +0.5 1,910,297 - +0.1 1,910,321 + +1.2 1,910,322 - -0.4 1,910,383 + -0.6 1,910,384 - -1.3 1,910,389 - -0.5 1,910,424 + +0.8 1,910,544 + +1.0 1,910,544 + -1.0 1,910,544 + -0.1 1,910,545 - +0.4 1,910,545 - +1.5 1,910,545 - -0.8 1,910,545 - -1.9 1,910,545 - -0.5 1,910,549 - -0.0 1,910,549 - +0.7 1,910,639 + RR42_RS09080 0.16 -0.0 1,910,651 + RR42_RS09080 0.17 +0.0 1,910,652 - RR42_RS09080 0.17 +1.1 1,910,748 + RR42_RS09080 0.25 +0.6 1,910,748 + RR42_RS09080 0.25 -0.4 1,910,748 + RR42_RS09080 0.25 +2.3 1,910,748 + RR42_RS09080 0.25 +0.0 1,910,749 - RR42_RS09080 0.25 -0.5 1,910,833 + RR42_RS09080 0.31 -0.4 1,910,834 - RR42_RS09080 0.31 +1.0 1,911,037 + RR42_RS09080 0.47 -0.0 1,911,327 + RR42_RS09080 0.69 -0.7 1,911,516 - RR42_RS09080 0.83 -0.1 1,911,516 - RR42_RS09080 0.83 -0.8 1,911,516 - RR42_RS09080 0.83 -0.0 1,911,557 - RR42_RS09080 0.86 +0.5 1,912,094 - RR42_RS09085 0.65 -2.5 1,912,152 + RR42_RS09085 0.76 -0.3 1,912,392 + +1.2 1,912,393 - +0.6 1,912,413 + +0.6 1,912,425 + +0.4 1,912,461 + -1.3 1,912,474 - -0.4 1,912,519 - -0.3 1,912,663 - RR42_RS09090 0.16 +0.6
Or see this region's nucleotide sequence