Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08780 and RR42_RS08785 overlap by 4 nucleotides RR42_RS08785 and RR42_RS08790 are separated by 157 nucleotides RR42_RS08790 and RR42_RS08795 are separated by 50 nucleotides RR42_RS08795 and RR42_RS08800 are separated by 140 nucleotides
RR42_RS08780: RR42_RS08780 - protease, at 1,840,826 to 1,841,743
_RS08780
RR42_RS08785: RR42_RS08785 - lipid carrier protein, at 1,841,740 to 1,842,192
_RS08785
RR42_RS08790: RR42_RS08790 - zinc-binding protein, at 1,842,350 to 1,842,799
_RS08790
RR42_RS08795: RR42_RS08795 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 1,842,850 to 1,843,428
_RS08795
RR42_RS08800: RR42_RS08800 - peptidylprolyl isomerase, at 1,843,569 to 1,844,315
_RS08800
Position (kb) 1842
1843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1842.104 kb on - strand, within RR42_RS08785 at 1842.234 kb on + strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.300 kb on + strand at 1842.390 kb on - strand at 1842.433 kb on + strand, within RR42_RS08790 at 1842.762 kb on + strand at 1842.763 kb on - strand at 1842.763 kb on - strand at 1842.805 kb on + strand at 1842.805 kb on + strand at 1842.830 kb on - strand at 1842.954 kb on + strand, within RR42_RS08795 at 1843.059 kb on - strand, within RR42_RS08795 at 1843.136 kb on - strand, within RR42_RS08795 at 1843.136 kb on - strand, within RR42_RS08795 at 1843.311 kb on + strand, within RR42_RS08795 at 1843.769 kb on - strand, within RR42_RS08800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,842,104 - RR42_RS08785 0.80 +0.5 1,842,234 + +2.1 1,842,235 - +1.0 1,842,235 - +0.8 1,842,235 - +0.1 1,842,300 + -1.9 1,842,390 - +0.1 1,842,433 + RR42_RS08790 0.18 -1.3 1,842,762 + -0.1 1,842,763 - -0.3 1,842,763 - -0.9 1,842,805 + -0.4 1,842,805 + -0.6 1,842,830 - +0.2 1,842,954 + RR42_RS08795 0.18 -3.2 1,843,059 - RR42_RS08795 0.36 -1.7 1,843,136 - RR42_RS08795 0.49 +0.2 1,843,136 - RR42_RS08795 0.49 -1.0 1,843,311 + RR42_RS08795 0.80 +0.1 1,843,769 - RR42_RS08800 0.27 -0.7
Or see this region's nucleotide sequence