Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08595 and RR42_RS08600 overlap by 4 nucleotides RR42_RS08600 and RR42_RS08605 overlap by 4 nucleotides RR42_RS08605 and RR42_RS08610 overlap by 4 nucleotides RR42_RS08610 and RR42_RS08615 overlap by 4 nucleotides
RR42_RS08595: RR42_RS08595 - virion morphogenesis protein, at 1,805,621 to 1,806,067
_RS08595
RR42_RS08600: RR42_RS08600 - tail protein, at 1,806,064 to 1,806,504
_RS08600
RR42_RS08605: RR42_RS08605 - hypothetical protein, at 1,806,501 to 1,807,010
_RS08605
RR42_RS08610: RR42_RS08610 - peptidoglycan-binding protein, at 1,807,007 to 1,807,813
_RS08610
RR42_RS08615: RR42_RS08615 - membrane protein, at 1,807,810 to 1,808,115
_RS08615
Position (kb) 1806
1807
1808 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1805.594 kb on + strand at 1805.611 kb on + strand at 1806.565 kb on - strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.873 kb on + strand, within RR42_RS08605 at 1806.926 kb on - strand, within RR42_RS08605 at 1807.011 kb on - strand at 1807.044 kb on + strand at 1807.068 kb on + strand at 1807.084 kb on - strand at 1807.098 kb on + strand, within RR42_RS08610 at 1807.098 kb on + strand, within RR42_RS08610 at 1807.099 kb on - strand, within RR42_RS08610 at 1807.146 kb on - strand, within RR42_RS08610 at 1807.146 kb on - strand, within RR42_RS08610 at 1807.222 kb on - strand, within RR42_RS08610 at 1807.222 kb on - strand, within RR42_RS08610 at 1807.239 kb on + strand, within RR42_RS08610 at 1807.240 kb on - strand, within RR42_RS08610 at 1807.340 kb on + strand, within RR42_RS08610 at 1807.341 kb on - strand, within RR42_RS08610 at 1807.341 kb on - strand, within RR42_RS08610 at 1807.341 kb on - strand, within RR42_RS08610 at 1807.366 kb on - strand, within RR42_RS08610 at 1807.389 kb on - strand, within RR42_RS08610 at 1807.449 kb on + strand, within RR42_RS08610 at 1807.556 kb on + strand, within RR42_RS08610 at 1807.899 kb on - strand, within RR42_RS08615 at 1807.899 kb on - strand, within RR42_RS08615
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,805,594 + +0.7 1,805,611 + +1.2 1,806,565 - RR42_RS08605 0.13 -1.8 1,806,595 + RR42_RS08605 0.18 -0.6 1,806,595 + RR42_RS08605 0.18 +0.1 1,806,596 - RR42_RS08605 0.19 -0.1 1,806,596 - RR42_RS08605 0.19 -2.9 1,806,732 - RR42_RS08605 0.45 +1.5 1,806,732 - RR42_RS08605 0.45 -2.6 1,806,732 - RR42_RS08605 0.45 -1.1 1,806,873 + RR42_RS08605 0.73 +0.0 1,806,926 - RR42_RS08605 0.83 +0.5 1,807,011 - +2.5 1,807,044 + +1.0 1,807,068 + -1.7 1,807,084 - +2.1 1,807,098 + RR42_RS08610 0.11 +0.7 1,807,098 + RR42_RS08610 0.11 +0.4 1,807,099 - RR42_RS08610 0.11 -1.1 1,807,146 - RR42_RS08610 0.17 -1.7 1,807,146 - RR42_RS08610 0.17 +0.9 1,807,222 - RR42_RS08610 0.27 -2.0 1,807,222 - RR42_RS08610 0.27 -1.1 1,807,239 + RR42_RS08610 0.29 +0.5 1,807,240 - RR42_RS08610 0.29 +0.7 1,807,340 + RR42_RS08610 0.41 -0.3 1,807,341 - RR42_RS08610 0.41 +0.4 1,807,341 - RR42_RS08610 0.41 -0.0 1,807,341 - RR42_RS08610 0.41 -1.6 1,807,366 - RR42_RS08610 0.44 +0.4 1,807,389 - RR42_RS08610 0.47 +1.2 1,807,449 + RR42_RS08610 0.55 -2.7 1,807,556 + RR42_RS08610 0.68 +0.9 1,807,899 - RR42_RS08615 0.29 +1.5 1,807,899 - RR42_RS08615 0.29 -0.2
Or see this region's nucleotide sequence