Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08560 and RR42_RS08565 are separated by 232 nucleotides RR42_RS08565 and RR42_RS08570 are separated by 12 nucleotides RR42_RS08570 and RR42_RS08575 are separated by 1 nucleotides RR42_RS08575 and RR42_RS08580 overlap by 8 nucleotides
RR42_RS08560: RR42_RS08560 - tail sheath protein, at 1,799,327 to 1,800,499
_RS08560
RR42_RS08565: RR42_RS08565 - hypothetical protein, at 1,800,732 to 1,801,331
_RS08565
RR42_RS08570: RR42_RS08570 - hypothetical protein, at 1,801,344 to 1,802,915
_RS08570
RR42_RS08575: RR42_RS08575 - tail protein, at 1,802,917 to 1,803,465
_RS08575
RR42_RS08580: RR42_RS08580 - baseplate assembly protein, at 1,803,458 to 1,804,366
_RS08580
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1800.637 kb on - strand at 1800.637 kb on - strand at 1801.129 kb on + strand, within RR42_RS08565 at 1801.294 kb on + strand at 1801.295 kb on - strand at 1801.295 kb on - strand at 1801.346 kb on - strand at 1801.348 kb on + strand at 1801.349 kb on - strand at 1801.349 kb on - strand at 1801.483 kb on + strand at 1801.483 kb on + strand at 1801.537 kb on + strand, within RR42_RS08570 at 1801.537 kb on + strand, within RR42_RS08570 at 1801.538 kb on - strand, within RR42_RS08570 at 1801.573 kb on + strand, within RR42_RS08570 at 1801.591 kb on + strand, within RR42_RS08570 at 1801.592 kb on - strand, within RR42_RS08570 at 1801.680 kb on + strand, within RR42_RS08570 at 1801.680 kb on + strand, within RR42_RS08570 at 1801.681 kb on - strand, within RR42_RS08570 at 1801.713 kb on - strand, within RR42_RS08570 at 1801.744 kb on + strand, within RR42_RS08570 at 1801.745 kb on - strand, within RR42_RS08570 at 1801.801 kb on + strand, within RR42_RS08570 at 1801.893 kb on + strand, within RR42_RS08570 at 1801.894 kb on - strand, within RR42_RS08570 at 1802.106 kb on - strand, within RR42_RS08570 at 1802.240 kb on - strand, within RR42_RS08570 at 1802.256 kb on + strand, within RR42_RS08570 at 1802.677 kb on + strand, within RR42_RS08570 at 1802.943 kb on - strand at 1803.284 kb on + strand, within RR42_RS08575 at 1803.589 kb on - strand, within RR42_RS08580 at 1803.640 kb on - strand, within RR42_RS08580 at 1803.640 kb on - strand, within RR42_RS08580 at 1803.702 kb on + strand, within RR42_RS08580 at 1803.702 kb on + strand, within RR42_RS08580 at 1803.703 kb on - strand, within RR42_RS08580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,800,637 - -1.3 1,800,637 - -0.9 1,801,129 + RR42_RS08565 0.66 -2.3 1,801,294 + +2.4 1,801,295 - -1.6 1,801,295 - -0.3 1,801,346 - +1.5 1,801,348 + -0.8 1,801,349 - +1.6 1,801,349 - +0.4 1,801,483 + -0.3 1,801,483 + -0.6 1,801,537 + RR42_RS08570 0.12 +2.3 1,801,537 + RR42_RS08570 0.12 -1.6 1,801,538 - RR42_RS08570 0.12 +0.7 1,801,573 + RR42_RS08570 0.15 -0.4 1,801,591 + RR42_RS08570 0.16 -1.0 1,801,592 - RR42_RS08570 0.16 -3.3 1,801,680 + RR42_RS08570 0.21 -0.3 1,801,680 + RR42_RS08570 0.21 +2.1 1,801,681 - RR42_RS08570 0.21 -0.1 1,801,713 - RR42_RS08570 0.23 -2.3 1,801,744 + RR42_RS08570 0.25 +1.2 1,801,745 - RR42_RS08570 0.26 +0.8 1,801,801 + RR42_RS08570 0.29 -1.8 1,801,893 + RR42_RS08570 0.35 +2.2 1,801,894 - RR42_RS08570 0.35 -1.1 1,802,106 - RR42_RS08570 0.48 -0.2 1,802,240 - RR42_RS08570 0.57 -3.4 1,802,256 + RR42_RS08570 0.58 -0.1 1,802,677 + RR42_RS08570 0.85 +0.4 1,802,943 - -0.8 1,803,284 + RR42_RS08575 0.67 -1.6 1,803,589 - RR42_RS08580 0.14 -3.7 1,803,640 - RR42_RS08580 0.20 -0.4 1,803,640 - RR42_RS08580 0.20 +0.2 1,803,702 + RR42_RS08580 0.27 -1.1 1,803,702 + RR42_RS08580 0.27 +0.6 1,803,703 - RR42_RS08580 0.27 +1.6
Or see this region's nucleotide sequence