Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08545 and RR42_RS08550 are separated by 94 nucleotides RR42_RS08550 and RR42_RS08555 are separated by 42 nucleotides RR42_RS08555 and RR42_RS08560 are separated by 22 nucleotides RR42_RS08560 and RR42_RS08565 are separated by 232 nucleotides RR42_RS08565 and RR42_RS08570 are separated by 12 nucleotides
RR42_RS08545: RR42_RS08545 - hypothetical protein, at 1,795,647 to 1,798,328
_RS08545
RR42_RS08550: RR42_RS08550 - tail fiber protein, at 1,798,423 to 1,798,752
_RS08550
RR42_RS08555: RR42_RS08555 - major tail tube protein, at 1,798,795 to 1,799,304
_RS08555
RR42_RS08560: RR42_RS08560 - tail sheath protein, at 1,799,327 to 1,800,499
_RS08560
RR42_RS08565: RR42_RS08565 - hypothetical protein, at 1,800,732 to 1,801,331
_RS08565
RR42_RS08570: RR42_RS08570 - hypothetical protein, at 1,801,344 to 1,802,915
_RS08570
Position (kb) 1799
1800
1801 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1798.464 kb on - strand, within RR42_RS08550 at 1798.777 kb on + strand at 1798.777 kb on + strand at 1798.797 kb on - strand at 1798.866 kb on - strand, within RR42_RS08555 at 1798.910 kb on + strand, within RR42_RS08555 at 1799.039 kb on + strand, within RR42_RS08555 at 1799.040 kb on - strand, within RR42_RS08555 at 1799.088 kb on - strand, within RR42_RS08555 at 1799.192 kb on + strand, within RR42_RS08555 at 1799.250 kb on - strand, within RR42_RS08555 at 1799.361 kb on + strand at 1799.419 kb on - strand at 1799.506 kb on - strand, within RR42_RS08560 at 1799.525 kb on - strand, within RR42_RS08560 at 1799.646 kb on + strand, within RR42_RS08560 at 1799.784 kb on + strand, within RR42_RS08560 at 1800.033 kb on + strand, within RR42_RS08560 at 1800.033 kb on + strand, within RR42_RS08560 at 1800.034 kb on - strand, within RR42_RS08560 at 1800.034 kb on - strand, within RR42_RS08560 at 1800.034 kb on - strand, within RR42_RS08560 at 1800.637 kb on - strand at 1800.637 kb on - strand at 1801.129 kb on + strand, within RR42_RS08565 at 1801.294 kb on + strand at 1801.295 kb on - strand at 1801.295 kb on - strand at 1801.346 kb on - strand at 1801.348 kb on + strand at 1801.349 kb on - strand at 1801.349 kb on - strand at 1801.483 kb on + strand at 1801.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,798,464 - RR42_RS08550 0.12 -0.2 1,798,777 + -0.9 1,798,777 + +0.4 1,798,797 - +1.1 1,798,866 - RR42_RS08555 0.14 +0.9 1,798,910 + RR42_RS08555 0.23 -2.6 1,799,039 + RR42_RS08555 0.48 +1.0 1,799,040 - RR42_RS08555 0.48 -0.7 1,799,088 - RR42_RS08555 0.57 -0.1 1,799,192 + RR42_RS08555 0.78 -3.0 1,799,250 - RR42_RS08555 0.89 -0.4 1,799,361 + -0.1 1,799,419 - -1.3 1,799,506 - RR42_RS08560 0.15 +1.0 1,799,525 - RR42_RS08560 0.17 -0.7 1,799,646 + RR42_RS08560 0.27 +0.2 1,799,784 + RR42_RS08560 0.39 -1.3 1,800,033 + RR42_RS08560 0.60 -0.1 1,800,033 + RR42_RS08560 0.60 +0.6 1,800,034 - RR42_RS08560 0.60 -0.0 1,800,034 - RR42_RS08560 0.60 +0.3 1,800,034 - RR42_RS08560 0.60 -3.3 1,800,637 - -1.3 1,800,637 - -0.9 1,801,129 + RR42_RS08565 0.66 -2.3 1,801,294 + +2.4 1,801,295 - -1.6 1,801,295 - -0.3 1,801,346 - +1.5 1,801,348 + -0.8 1,801,349 - +1.6 1,801,349 - +0.4 1,801,483 + -0.3 1,801,483 + -0.6
Or see this region's nucleotide sequence