Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08420 and RR42_RS08425 are separated by 982 nucleotides RR42_RS08425 and RR42_RS08430 are separated by 427 nucleotides
RR42_RS08420: RR42_RS08420 - hypothetical protein, at 1,777,325 to 1,777,585
_RS08420
RR42_RS08425: RR42_RS08425 - hypothetical protein, at 1,778,568 to 1,779,206
_RS08425
RR42_RS08430: RR42_RS08430 - hypothetical protein, at 1,779,634 to 1,780,215
_RS08430
Position (kb)
1778
1779
1780 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1777.622 kb on - strand at 1777.622 kb on - strand at 1777.634 kb on - strand at 1777.641 kb on + strand at 1777.642 kb on - strand at 1777.711 kb on + strand at 1777.714 kb on + strand at 1777.889 kb on + strand at 1777.890 kb on - strand at 1777.890 kb on - strand at 1777.924 kb on + strand at 1777.925 kb on - strand at 1778.074 kb on + strand at 1778.155 kb on + strand at 1778.156 kb on - strand at 1778.156 kb on - strand at 1778.156 kb on - strand at 1778.156 kb on - strand at 1778.207 kb on + strand at 1778.208 kb on - strand at 1778.239 kb on - strand at 1778.239 kb on - strand at 1778.280 kb on + strand at 1778.280 kb on + strand at 1778.280 kb on + strand at 1778.318 kb on + strand at 1778.404 kb on + strand at 1778.405 kb on - strand at 1778.405 kb on - strand at 1778.405 kb on - strand at 1778.540 kb on + strand at 1778.540 kb on + strand at 1778.551 kb on - strand at 1778.857 kb on + strand, within RR42_RS08425 at 1779.128 kb on + strand, within RR42_RS08425 at 1779.150 kb on - strand at 1779.382 kb on - strand at 1779.382 kb on - strand at 1779.470 kb on + strand at 1779.471 kb on - strand at 1779.511 kb on + strand at 1779.514 kb on + strand at 1779.514 kb on + strand at 1779.520 kb on + strand at 1779.565 kb on + strand at 1779.679 kb on + strand at 1779.680 kb on - strand at 1779.681 kb on + strand at 1779.682 kb on - strand at 1779.682 kb on - strand at 1779.682 kb on - strand at 1779.778 kb on + strand, within RR42_RS08430 at 1779.779 kb on - strand, within RR42_RS08430 at 1779.875 kb on + strand, within RR42_RS08430 at 1779.930 kb on + strand, within RR42_RS08430 at 1779.930 kb on + strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.958 kb on - strand, within RR42_RS08430 at 1780.010 kb on + strand, within RR42_RS08430 at 1780.014 kb on - strand, within RR42_RS08430 at 1780.039 kb on + strand, within RR42_RS08430 at 1780.056 kb on - strand, within RR42_RS08430 at 1780.066 kb on + strand, within RR42_RS08430 at 1780.083 kb on + strand, within RR42_RS08430 at 1780.083 kb on + strand, within RR42_RS08430 at 1780.084 kb on - strand, within RR42_RS08430 at 1780.084 kb on - strand, within RR42_RS08430 at 1780.167 kb on + strand at 1780.168 kb on - strand at 1780.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,777,622 - -1.7 1,777,622 - -2.9 1,777,634 - -0.1 1,777,641 + -1.9 1,777,642 - -0.3 1,777,711 + -0.0 1,777,714 + -0.5 1,777,889 + -1.6 1,777,890 - -0.0 1,777,890 - -1.0 1,777,924 + +1.1 1,777,925 - +0.7 1,778,074 + +0.9 1,778,155 + +1.1 1,778,156 - -2.3 1,778,156 - +0.7 1,778,156 - -1.6 1,778,156 - +2.1 1,778,207 + +0.2 1,778,208 - -0.6 1,778,239 - -1.1 1,778,239 - -0.9 1,778,280 + +0.1 1,778,280 + -1.6 1,778,280 + +3.1 1,778,318 + -0.6 1,778,404 + +0.7 1,778,405 - -2.9 1,778,405 - -1.6 1,778,405 - -0.9 1,778,540 + -1.6 1,778,540 + +0.3 1,778,551 - -1.2 1,778,857 + RR42_RS08425 0.45 -0.9 1,779,128 + RR42_RS08425 0.88 -1.9 1,779,150 - -0.2 1,779,382 - -1.6 1,779,382 - -0.0 1,779,470 + +1.3 1,779,471 - -0.9 1,779,511 + +0.1 1,779,514 + +1.2 1,779,514 + -0.0 1,779,520 + +1.7 1,779,565 + +0.3 1,779,679 + -0.5 1,779,680 - -1.3 1,779,681 + -1.3 1,779,682 - -0.1 1,779,682 - +1.0 1,779,682 - +0.6 1,779,778 + RR42_RS08430 0.25 -0.3 1,779,779 - RR42_RS08430 0.25 -0.1 1,779,875 + RR42_RS08430 0.41 -2.9 1,779,930 + RR42_RS08430 0.51 +0.2 1,779,930 + RR42_RS08430 0.51 +0.8 1,779,931 - RR42_RS08430 0.51 -1.2 1,779,931 - RR42_RS08430 0.51 -0.4 1,779,931 - RR42_RS08430 0.51 -0.6 1,779,958 - RR42_RS08430 0.56 -0.9 1,780,010 + RR42_RS08430 0.65 -0.2 1,780,014 - RR42_RS08430 0.65 +0.1 1,780,039 + RR42_RS08430 0.70 -0.0 1,780,056 - RR42_RS08430 0.73 -2.4 1,780,066 + RR42_RS08430 0.74 +0.7 1,780,083 + RR42_RS08430 0.77 -0.3 1,780,083 + RR42_RS08430 0.77 +0.1 1,780,084 - RR42_RS08430 0.77 -0.6 1,780,084 - RR42_RS08430 0.77 -0.3 1,780,167 + -2.6 1,780,168 - -1.0 1,780,188 - +2.7
Or see this region's nucleotide sequence