Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08345 and RR42_RS08350 are separated by 1 nucleotides RR42_RS08350 and RR42_RS08355 are separated by 97 nucleotides
RR42_RS08345: RR42_RS08345 - baseplate protein, at 1,765,485 to 1,766,609
_RS08345
RR42_RS08350: RR42_RS08350 - hypothetical protein, at 1,766,611 to 1,767,294
_RS08350
RR42_RS08355: RR42_RS08355 - hypothetical protein, at 1,767,392 to 1,768,036
_RS08355
Position (kb)
1766
1767
1768 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1765.741 kb on + strand, within RR42_RS08345 at 1765.774 kb on - strand, within RR42_RS08345 at 1765.876 kb on - strand, within RR42_RS08345 at 1766.020 kb on - strand, within RR42_RS08345 at 1766.197 kb on - strand, within RR42_RS08345 at 1766.197 kb on - strand, within RR42_RS08345 at 1766.478 kb on + strand, within RR42_RS08345 at 1766.597 kb on + strand at 1766.657 kb on - strand at 1766.657 kb on - strand at 1766.657 kb on - strand at 1766.746 kb on + strand, within RR42_RS08350 at 1766.876 kb on - strand, within RR42_RS08350 at 1767.293 kb on - strand at 1767.377 kb on + strand at 1767.377 kb on + strand at 1767.377 kb on + strand at 1767.378 kb on - strand at 1767.378 kb on - strand at 1767.378 kb on - strand at 1767.378 kb on - strand at 1767.382 kb on - strand at 1767.382 kb on - strand at 1767.382 kb on - strand at 1767.395 kb on + strand at 1767.395 kb on + strand at 1767.396 kb on - strand at 1767.551 kb on + strand, within RR42_RS08355 at 1767.608 kb on + strand, within RR42_RS08355 at 1767.608 kb on + strand, within RR42_RS08355 at 1767.644 kb on + strand, within RR42_RS08355 at 1767.645 kb on - strand, within RR42_RS08355 at 1767.695 kb on + strand, within RR42_RS08355 at 1767.696 kb on - strand, within RR42_RS08355 at 1767.776 kb on + strand, within RR42_RS08355 at 1767.864 kb on - strand, within RR42_RS08355 at 1767.998 kb on + strand at 1768.168 kb on + strand at 1768.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,765,741 + RR42_RS08345 0.23 +1.5 1,765,774 - RR42_RS08345 0.26 +0.9 1,765,876 - RR42_RS08345 0.35 -0.7 1,766,020 - RR42_RS08345 0.48 +0.9 1,766,197 - RR42_RS08345 0.63 +0.9 1,766,197 - RR42_RS08345 0.63 +0.4 1,766,478 + RR42_RS08345 0.88 -2.7 1,766,597 + +0.7 1,766,657 - +0.4 1,766,657 - -0.1 1,766,657 - +0.4 1,766,746 + RR42_RS08350 0.20 -2.5 1,766,876 - RR42_RS08350 0.39 -3.3 1,767,293 - +0.9 1,767,377 + +3.3 1,767,377 + -1.4 1,767,377 + -2.7 1,767,378 - -0.0 1,767,378 - -0.9 1,767,378 - -0.3 1,767,378 - -0.6 1,767,382 - -0.8 1,767,382 - -0.8 1,767,382 - -2.7 1,767,395 + +0.7 1,767,395 + -1.3 1,767,396 - -0.6 1,767,551 + RR42_RS08355 0.25 +1.2 1,767,608 + RR42_RS08355 0.33 -0.7 1,767,608 + RR42_RS08355 0.33 -1.2 1,767,644 + RR42_RS08355 0.39 -0.3 1,767,645 - RR42_RS08355 0.39 -2.3 1,767,695 + RR42_RS08355 0.47 -0.9 1,767,696 - RR42_RS08355 0.47 -2.4 1,767,776 + RR42_RS08355 0.60 +0.2 1,767,864 - RR42_RS08355 0.73 +0.5 1,767,998 + +1.4 1,768,168 + -0.8 1,768,169 - -0.4
Or see this region's nucleotide sequence