Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07990 and RR42_RS07995 overlap by 4 nucleotides RR42_RS07995 and RR42_RS08000 are separated by 60 nucleotides RR42_RS08000 and RR42_RS08005 are separated by 0 nucleotides RR42_RS08005 and RR42_RS08010 are separated by 32 nucleotides
RR42_RS07990: RR42_RS07990 - integrase, at 1,717,192 to 1,718,196
_RS07990
RR42_RS07995: RR42_RS07995 - hypothetical protein, at 1,718,193 to 1,718,519
_RS07995
RR42_RS08000: RR42_RS08000 - hypothetical protein, at 1,718,580 to 1,718,795
_RS08000
RR42_RS08005: RR42_RS08005 - hypothetical protein, at 1,718,796 to 1,719,347
_RS08005
RR42_RS08010: RR42_RS08010 - hypothetical protein, at 1,719,380 to 1,719,634
_RS08010
Position (kb) 1718
1719 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1717.203 kb on + strand at 1717.381 kb on + strand, within RR42_RS07990 at 1717.381 kb on + strand, within RR42_RS07990 at 1717.563 kb on - strand, within RR42_RS07990 at 1717.650 kb on - strand, within RR42_RS07990 at 1718.071 kb on - strand, within RR42_RS07990 at 1718.071 kb on - strand, within RR42_RS07990 at 1718.152 kb on + strand at 1718.153 kb on - strand at 1718.155 kb on + strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.156 kb on - strand at 1718.411 kb on - strand, within RR42_RS07995 at 1718.595 kb on + strand at 1718.626 kb on + strand, within RR42_RS08000 at 1718.808 kb on + strand at 1718.812 kb on + strand at 1718.863 kb on + strand, within RR42_RS08005 at 1718.863 kb on + strand, within RR42_RS08005 at 1718.863 kb on + strand, within RR42_RS08005 at 1719.142 kb on + strand, within RR42_RS08005 at 1719.234 kb on + strand, within RR42_RS08005 at 1719.401 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,717,203 + +1.0 1,717,381 + RR42_RS07990 0.19 +0.2 1,717,381 + RR42_RS07990 0.19 -0.8 1,717,563 - RR42_RS07990 0.37 -3.0 1,717,650 - RR42_RS07990 0.46 -1.2 1,718,071 - RR42_RS07990 0.87 +0.7 1,718,071 - RR42_RS07990 0.87 +1.3 1,718,152 + -0.7 1,718,153 - +0.4 1,718,155 + +1.2 1,718,156 - +1.0 1,718,156 - +0.4 1,718,156 - -3.4 1,718,156 - -2.2 1,718,411 - RR42_RS07995 0.67 -1.4 1,718,595 + -0.8 1,718,626 + RR42_RS08000 0.21 -0.1 1,718,808 + -0.3 1,718,812 + -0.5 1,718,863 + RR42_RS08005 0.12 -0.6 1,718,863 + RR42_RS08005 0.12 +0.8 1,718,863 + RR42_RS08005 0.12 +1.0 1,719,142 + RR42_RS08005 0.63 -1.3 1,719,234 + RR42_RS08005 0.79 -0.8 1,719,401 + +0.8
Or see this region's nucleotide sequence