Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07860 and RR42_RS07865 are separated by 266 nucleotides RR42_RS07865 and RR42_RS07870 are separated by 141 nucleotides RR42_RS07870 and RR42_RS07875 are separated by 299 nucleotides
RR42_RS07860: RR42_RS07860 - acetyltransferase, at 1,686,529 to 1,687,110
_RS07860
RR42_RS07865: RR42_RS07865 - carboxymuconolactone decarboxylase, at 1,687,377 to 1,687,898
_RS07865
RR42_RS07870: RR42_RS07870 - alkyl hydroperoxide reductase, at 1,688,040 to 1,688,588
_RS07870
RR42_RS07875: RR42_RS07875 - citrate-proton symporter, at 1,688,888 to 1,690,189
_RS07875
Position (kb)
1688
1689 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1687.089 kb on + strand at 1687.090 kb on - strand at 1687.090 kb on - strand at 1687.090 kb on - strand at 1687.090 kb on - strand at 1687.129 kb on + strand at 1687.130 kb on - strand at 1687.130 kb on - strand at 1687.147 kb on + strand at 1687.148 kb on - strand at 1687.148 kb on - strand at 1687.483 kb on + strand, within RR42_RS07865 at 1687.537 kb on + strand, within RR42_RS07865 at 1687.537 kb on + strand, within RR42_RS07865 at 1687.537 kb on + strand, within RR42_RS07865 at 1687.538 kb on - strand, within RR42_RS07865 at 1687.579 kb on + strand, within RR42_RS07865 at 1687.580 kb on - strand, within RR42_RS07865 at 1687.580 kb on - strand, within RR42_RS07865 at 1687.858 kb on + strand at 1688.041 kb on + strand at 1688.041 kb on + strand at 1688.042 kb on - strand at 1688.109 kb on - strand, within RR42_RS07870 at 1688.196 kb on - strand, within RR42_RS07870 at 1688.196 kb on - strand, within RR42_RS07870 at 1688.449 kb on + strand, within RR42_RS07870 at 1688.449 kb on + strand, within RR42_RS07870 at 1688.449 kb on + strand, within RR42_RS07870 at 1688.449 kb on + strand, within RR42_RS07870 at 1688.450 kb on - strand, within RR42_RS07870 at 1688.450 kb on - strand, within RR42_RS07870 at 1688.450 kb on - strand, within RR42_RS07870 at 1688.588 kb on + strand at 1688.588 kb on + strand at 1688.588 kb on + strand at 1688.711 kb on + strand at 1688.718 kb on + strand at 1688.861 kb on - strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.916 kb on + strand at 1688.917 kb on - strand at 1688.917 kb on - strand at 1689.079 kb on + strand, within RR42_RS07875 at 1689.081 kb on + strand, within RR42_RS07875 at 1689.081 kb on + strand, within RR42_RS07875 at 1689.081 kb on + strand, within RR42_RS07875 at 1689.082 kb on - strand, within RR42_RS07875 at 1689.082 kb on - strand, within RR42_RS07875 at 1689.082 kb on - strand, within RR42_RS07875 at 1689.082 kb on - strand, within RR42_RS07875 at 1689.156 kb on + strand, within RR42_RS07875 at 1689.156 kb on + strand, within RR42_RS07875 at 1689.157 kb on - strand, within RR42_RS07875 at 1689.157 kb on - strand, within RR42_RS07875 at 1689.157 kb on - strand, within RR42_RS07875 at 1689.157 kb on - strand, within RR42_RS07875 at 1689.165 kb on + strand, within RR42_RS07875 at 1689.420 kb on + strand, within RR42_RS07875 at 1689.420 kb on + strand, within RR42_RS07875 at 1689.420 kb on + strand, within RR42_RS07875 at 1689.421 kb on - strand, within RR42_RS07875 at 1689.421 kb on - strand, within RR42_RS07875 at 1689.421 kb on - strand, within RR42_RS07875 at 1689.435 kb on + strand, within RR42_RS07875 at 1689.435 kb on + strand, within RR42_RS07875 at 1689.435 kb on + strand, within RR42_RS07875 at 1689.435 kb on + strand, within RR42_RS07875 at 1689.435 kb on + strand, within RR42_RS07875 at 1689.436 kb on - strand, within RR42_RS07875 at 1689.436 kb on - strand, within RR42_RS07875 at 1689.436 kb on - strand, within RR42_RS07875 at 1689.436 kb on - strand, within RR42_RS07875 at 1689.510 kb on + strand, within RR42_RS07875 at 1689.510 kb on + strand, within RR42_RS07875 at 1689.510 kb on + strand, within RR42_RS07875 at 1689.511 kb on - strand, within RR42_RS07875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,687,089 + +0.6 1,687,090 - -2.2 1,687,090 - -0.0 1,687,090 - -1.6 1,687,090 - +0.2 1,687,129 + +0.2 1,687,130 - -0.4 1,687,130 - +0.4 1,687,147 + +0.4 1,687,148 - -0.0 1,687,148 - -0.0 1,687,483 + RR42_RS07865 0.20 -3.5 1,687,537 + RR42_RS07865 0.31 +0.8 1,687,537 + RR42_RS07865 0.31 -1.0 1,687,537 + RR42_RS07865 0.31 +0.1 1,687,538 - RR42_RS07865 0.31 +0.5 1,687,579 + RR42_RS07865 0.39 -2.9 1,687,580 - RR42_RS07865 0.39 +0.7 1,687,580 - RR42_RS07865 0.39 +0.7 1,687,858 + +1.2 1,688,041 + +0.8 1,688,041 + +0.3 1,688,042 - +0.2 1,688,109 - RR42_RS07870 0.13 +0.7 1,688,196 - RR42_RS07870 0.28 +1.9 1,688,196 - RR42_RS07870 0.28 +3.6 1,688,449 + RR42_RS07870 0.74 +1.2 1,688,449 + RR42_RS07870 0.74 +2.4 1,688,449 + RR42_RS07870 0.74 +5.9 1,688,449 + RR42_RS07870 0.74 +2.4 1,688,450 - RR42_RS07870 0.75 +1.6 1,688,450 - RR42_RS07870 0.75 +3.2 1,688,450 - RR42_RS07870 0.75 +3.3 1,688,588 + +1.5 1,688,588 + +2.3 1,688,588 + +0.8 1,688,711 + +2.1 1,688,718 + +1.9 1,688,861 - -0.7 1,688,916 + +0.2 1,688,916 + -2.7 1,688,916 + -0.4 1,688,916 + +0.3 1,688,916 + -0.1 1,688,916 + +0.4 1,688,916 + +1.1 1,688,916 + -0.4 1,688,916 + +0.8 1,688,916 + +1.0 1,688,917 - +0.8 1,688,917 - -0.6 1,689,079 + RR42_RS07875 0.15 +0.4 1,689,081 + RR42_RS07875 0.15 +1.2 1,689,081 + RR42_RS07875 0.15 +0.7 1,689,081 + RR42_RS07875 0.15 -0.4 1,689,082 - RR42_RS07875 0.15 -0.2 1,689,082 - RR42_RS07875 0.15 -1.2 1,689,082 - RR42_RS07875 0.15 +0.1 1,689,082 - RR42_RS07875 0.15 -1.8 1,689,156 + RR42_RS07875 0.21 +0.8 1,689,156 + RR42_RS07875 0.21 -0.2 1,689,157 - RR42_RS07875 0.21 +1.0 1,689,157 - RR42_RS07875 0.21 -0.2 1,689,157 - RR42_RS07875 0.21 -1.2 1,689,157 - RR42_RS07875 0.21 +0.4 1,689,165 + RR42_RS07875 0.21 +1.1 1,689,420 + RR42_RS07875 0.41 -3.6 1,689,420 + RR42_RS07875 0.41 +1.5 1,689,420 + RR42_RS07875 0.41 -0.6 1,689,421 - RR42_RS07875 0.41 +1.0 1,689,421 - RR42_RS07875 0.41 +1.6 1,689,421 - RR42_RS07875 0.41 -1.5 1,689,435 + RR42_RS07875 0.42 -2.2 1,689,435 + RR42_RS07875 0.42 +0.6 1,689,435 + RR42_RS07875 0.42 -0.7 1,689,435 + RR42_RS07875 0.42 +0.4 1,689,435 + RR42_RS07875 0.42 +0.8 1,689,436 - RR42_RS07875 0.42 -1.2 1,689,436 - RR42_RS07875 0.42 +0.4 1,689,436 - RR42_RS07875 0.42 -2.5 1,689,436 - RR42_RS07875 0.42 +1.0 1,689,510 + RR42_RS07875 0.48 -1.1 1,689,510 + RR42_RS07875 0.48 +0.6 1,689,510 + RR42_RS07875 0.48 -1.0 1,689,511 - RR42_RS07875 0.48 +0.2
Or see this region's nucleotide sequence