Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07770 and RR42_RS07775 are separated by 59 nucleotides RR42_RS07775 and RR42_RS07780 are separated by 363 nucleotides RR42_RS07780 and RR42_RS07785 are separated by 195 nucleotides
RR42_RS07770: RR42_RS07770 - hypothetical protein, at 1,663,956 to 1,664,780
_RS07770
RR42_RS07775: RR42_RS07775 - ABC transporter substrate-binding protein, at 1,664,840 to 1,665,733
_RS07775
RR42_RS07780: RR42_RS07780 - hypothetical protein, at 1,666,097 to 1,666,279
_RS07780
RR42_RS07785: RR42_RS07785 - hypothetical protein, at 1,666,475 to 1,666,762
_RS07785
Position (kb)
1664
1665
1666 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1663.914 kb on + strand at 1663.914 kb on + strand at 1663.927 kb on + strand at 1664.011 kb on - strand at 1664.092 kb on + strand, within RR42_RS07770 at 1664.093 kb on - strand, within RR42_RS07770 at 1664.093 kb on - strand, within RR42_RS07770 at 1664.197 kb on + strand, within RR42_RS07770 at 1664.205 kb on + strand, within RR42_RS07770 at 1664.205 kb on + strand, within RR42_RS07770 at 1664.206 kb on - strand, within RR42_RS07770 at 1664.478 kb on + strand, within RR42_RS07770 at 1664.479 kb on - strand, within RR42_RS07770 at 1664.726 kb on - strand at 1664.766 kb on + strand at 1664.842 kb on - strand at 1664.883 kb on - strand at 1664.953 kb on - strand, within RR42_RS07775 at 1664.953 kb on - strand, within RR42_RS07775 at 1665.049 kb on - strand, within RR42_RS07775 at 1665.049 kb on - strand, within RR42_RS07775 at 1665.189 kb on + strand, within RR42_RS07775 at 1665.190 kb on - strand, within RR42_RS07775 at 1665.190 kb on - strand, within RR42_RS07775 at 1665.297 kb on - strand, within RR42_RS07775 at 1665.313 kb on + strand, within RR42_RS07775 at 1665.355 kb on - strand, within RR42_RS07775 at 1665.429 kb on + strand, within RR42_RS07775 at 1665.429 kb on + strand, within RR42_RS07775 at 1665.429 kb on + strand, within RR42_RS07775 at 1665.429 kb on + strand, within RR42_RS07775 at 1665.430 kb on - strand, within RR42_RS07775 at 1665.430 kb on - strand, within RR42_RS07775 at 1665.440 kb on + strand, within RR42_RS07775 at 1665.480 kb on + strand, within RR42_RS07775 at 1665.490 kb on + strand, within RR42_RS07775 at 1665.663 kb on + strand at 1665.706 kb on + strand at 1665.790 kb on + strand at 1665.790 kb on + strand at 1665.791 kb on - strand at 1665.794 kb on - strand at 1665.794 kb on - strand at 1665.801 kb on + strand at 1665.801 kb on + strand at 1665.802 kb on - strand at 1666.020 kb on + strand at 1666.020 kb on + strand at 1666.021 kb on - strand at 1666.024 kb on + strand at 1666.025 kb on - strand at 1666.152 kb on - strand, within RR42_RS07780 at 1666.236 kb on + strand, within RR42_RS07780 at 1666.237 kb on - strand, within RR42_RS07780 at 1666.349 kb on + strand at 1666.349 kb on + strand at 1666.350 kb on - strand at 1666.350 kb on - strand at 1666.350 kb on - strand at 1666.350 kb on - strand at 1666.386 kb on - strand at 1666.499 kb on - strand at 1666.531 kb on + strand, within RR42_RS07785 at 1666.570 kb on - strand, within RR42_RS07785 at 1666.573 kb on + strand, within RR42_RS07785 at 1666.574 kb on - strand, within RR42_RS07785 at 1666.624 kb on - strand, within RR42_RS07785 at 1666.687 kb on + strand, within RR42_RS07785 at 1666.689 kb on + strand, within RR42_RS07785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,663,914 + +0.1 1,663,914 + -0.9 1,663,927 + +0.1 1,664,011 - -0.1 1,664,092 + RR42_RS07770 0.16 -3.0 1,664,093 - RR42_RS07770 0.17 -0.0 1,664,093 - RR42_RS07770 0.17 -1.3 1,664,197 + RR42_RS07770 0.29 -2.2 1,664,205 + RR42_RS07770 0.30 +1.3 1,664,205 + RR42_RS07770 0.30 +0.3 1,664,206 - RR42_RS07770 0.30 -2.6 1,664,478 + RR42_RS07770 0.63 -1.1 1,664,479 - RR42_RS07770 0.63 -0.3 1,664,726 - -0.3 1,664,766 + +0.4 1,664,842 - +0.4 1,664,883 - +0.4 1,664,953 - RR42_RS07775 0.13 +2.1 1,664,953 - RR42_RS07775 0.13 +0.2 1,665,049 - RR42_RS07775 0.23 -0.2 1,665,049 - RR42_RS07775 0.23 -1.5 1,665,189 + RR42_RS07775 0.39 -0.5 1,665,190 - RR42_RS07775 0.39 +0.8 1,665,190 - RR42_RS07775 0.39 -0.8 1,665,297 - RR42_RS07775 0.51 +0.3 1,665,313 + RR42_RS07775 0.53 -0.2 1,665,355 - RR42_RS07775 0.58 +0.6 1,665,429 + RR42_RS07775 0.66 -0.2 1,665,429 + RR42_RS07775 0.66 +0.2 1,665,429 + RR42_RS07775 0.66 -0.1 1,665,429 + RR42_RS07775 0.66 +0.8 1,665,430 - RR42_RS07775 0.66 -2.0 1,665,430 - RR42_RS07775 0.66 +1.0 1,665,440 + RR42_RS07775 0.67 +0.1 1,665,480 + RR42_RS07775 0.72 +0.3 1,665,490 + RR42_RS07775 0.73 +0.4 1,665,663 + -0.0 1,665,706 + -1.8 1,665,790 + -0.6 1,665,790 + +0.9 1,665,791 - -1.5 1,665,794 - -1.5 1,665,794 - -1.5 1,665,801 + -0.5 1,665,801 + -0.3 1,665,802 - +1.8 1,666,020 + +0.0 1,666,020 + -0.6 1,666,021 - +1.4 1,666,024 + -1.8 1,666,025 - +0.1 1,666,152 - RR42_RS07780 0.30 -0.3 1,666,236 + RR42_RS07780 0.76 +0.7 1,666,237 - RR42_RS07780 0.77 +1.5 1,666,349 + -2.0 1,666,349 + +0.9 1,666,350 - +0.9 1,666,350 - -0.4 1,666,350 - -1.6 1,666,350 - -0.1 1,666,386 - -0.1 1,666,499 - -1.0 1,666,531 + RR42_RS07785 0.19 -2.0 1,666,570 - RR42_RS07785 0.33 +0.1 1,666,573 + RR42_RS07785 0.34 -0.2 1,666,574 - RR42_RS07785 0.34 -0.2 1,666,624 - RR42_RS07785 0.52 +1.4 1,666,687 + RR42_RS07785 0.74 -0.8 1,666,689 + RR42_RS07785 0.74 -1.3
Or see this region's nucleotide sequence