Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS07540

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS07530 and RR42_RS07535 are separated by 110 nucleotidesRR42_RS07535 and RR42_RS07540 are separated by 4 nucleotidesRR42_RS07540 and RR42_RS07545 overlap by 4 nucleotidesRR42_RS07545 and RR42_RS07550 overlap by 4 nucleotides RR42_RS07530: RR42_RS07530 - ABC transporter permease, at 1,609,529 to 1,610,710 _RS07530 RR42_RS07535: RR42_RS07535 - ABC transporter permease, at 1,610,821 to 1,611,687 _RS07535 RR42_RS07540: RR42_RS07540 - ABC transporter permease, at 1,611,692 to 1,612,717 _RS07540 RR42_RS07545: RR42_RS07545 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,612,714 to 1,613,562 _RS07545 RR42_RS07550: RR42_RS07550 - ABC transporter, at 1,613,559 to 1,614,278 _RS07550 Position (kb) 1611 1612 1613Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1610.716 kb on + strandat 1610.856 kb on + strandat 1610.856 kb on + strandat 1610.856 kb on + strandat 1610.866 kb on + strandat 1610.953 kb on + strand, within RR42_RS07535at 1610.953 kb on + strand, within RR42_RS07535at 1610.953 kb on + strand, within RR42_RS07535at 1610.953 kb on + strand, within RR42_RS07535at 1610.953 kb on + strand, within RR42_RS07535at 1610.953 kb on + strand, within RR42_RS07535at 1610.954 kb on - strand, within RR42_RS07535at 1610.954 kb on - strand, within RR42_RS07535at 1611.079 kb on + strand, within RR42_RS07535at 1611.080 kb on - strand, within RR42_RS07535at 1611.080 kb on - strand, within RR42_RS07535at 1611.080 kb on - strand, within RR42_RS07535at 1611.080 kb on - strand, within RR42_RS07535at 1611.085 kb on + strand, within RR42_RS07535at 1611.086 kb on - strand, within RR42_RS07535at 1611.121 kb on + strand, within RR42_RS07535at 1611.122 kb on - strand, within RR42_RS07535at 1611.176 kb on + strand, within RR42_RS07535at 1611.176 kb on + strand, within RR42_RS07535at 1611.176 kb on + strand, within RR42_RS07535at 1611.176 kb on + strand, within RR42_RS07535at 1611.176 kb on + strand, within RR42_RS07535at 1611.177 kb on - strand, within RR42_RS07535at 1611.177 kb on - strand, within RR42_RS07535at 1611.177 kb on - strand, within RR42_RS07535at 1611.239 kb on - strand, within RR42_RS07535at 1611.248 kb on - strand, within RR42_RS07535at 1611.295 kb on + strand, within RR42_RS07535at 1611.296 kb on - strand, within RR42_RS07535at 1611.296 kb on - strand, within RR42_RS07535at 1611.394 kb on + strand, within RR42_RS07535at 1611.583 kb on - strand, within RR42_RS07535at 1611.629 kb on - strandat 1611.704 kb on - strandat 1611.807 kb on + strand, within RR42_RS07540at 1611.808 kb on - strand, within RR42_RS07540at 1611.906 kb on - strand, within RR42_RS07540at 1611.938 kb on + strand, within RR42_RS07540at 1612.007 kb on - strand, within RR42_RS07540at 1612.085 kb on + strand, within RR42_RS07540at 1612.085 kb on + strand, within RR42_RS07540at 1612.099 kb on + strand, within RR42_RS07540at 1612.124 kb on + strand, within RR42_RS07540at 1612.124 kb on + strand, within RR42_RS07540at 1612.124 kb on + strand, within RR42_RS07540at 1612.124 kb on - strand, within RR42_RS07540at 1612.125 kb on - strand, within RR42_RS07540at 1612.125 kb on - strand, within RR42_RS07540at 1612.125 kb on - strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.172 kb on + strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.173 kb on - strand, within RR42_RS07540at 1612.174 kb on + strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.175 kb on - strand, within RR42_RS07540at 1612.179 kb on - strand, within RR42_RS07540at 1612.179 kb on - strand, within RR42_RS07540at 1612.244 kb on + strand, within RR42_RS07540at 1612.245 kb on - strand, within RR42_RS07540at 1612.294 kb on - strand, within RR42_RS07540at 1612.340 kb on - strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.376 kb on + strand, within RR42_RS07540at 1612.377 kb on - strand, within RR42_RS07540at 1612.377 kb on - strand, within RR42_RS07540at 1612.434 kb on - strand, within RR42_RS07540at 1612.439 kb on + strand, within RR42_RS07540at 1612.439 kb on + strand, within RR42_RS07540at 1612.439 kb on + strand, within RR42_RS07540at 1612.440 kb on - strand, within RR42_RS07540at 1612.440 kb on - strand, within RR42_RS07540at 1612.881 kb on + strand, within RR42_RS07545at 1612.881 kb on + strand, within RR42_RS07545at 1612.881 kb on + strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1612.882 kb on - strand, within RR42_RS07545at 1613.004 kb on + strand, within RR42_RS07545at 1613.005 kb on - strand, within RR42_RS07545at 1613.005 kb on - strand, within RR42_RS07545at 1613.465 kb on - strand, within RR42_RS07545at 1613.465 kb on - strand, within RR42_RS07545at 1613.545 kb on - strandat 1613.607 kb on + strandat 1613.608 kb on - strandat 1613.608 kb on - strandat 1613.711 kb on + strand, within RR42_RS07550at 1613.711 kb on + strand, within RR42_RS07550at 1613.712 kb on - strand, within RR42_RS07550

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,610,716 + +0.8
1,610,856 + -0.4
1,610,856 + -0.1
1,610,856 + -0.6
1,610,866 + -0.1
1,610,953 + RR42_RS07535 0.15 +0.3
1,610,953 + RR42_RS07535 0.15 -1.9
1,610,953 + RR42_RS07535 0.15 -0.6
1,610,953 + RR42_RS07535 0.15 -2.6
1,610,953 + RR42_RS07535 0.15 -2.5
1,610,953 + RR42_RS07535 0.15 -0.0
1,610,954 - RR42_RS07535 0.15 +0.6
1,610,954 - RR42_RS07535 0.15 +0.1
1,611,079 + RR42_RS07535 0.30 +0.8
1,611,080 - RR42_RS07535 0.30 +0.8
1,611,080 - RR42_RS07535 0.30 -2.5
1,611,080 - RR42_RS07535 0.30 +0.2
1,611,080 - RR42_RS07535 0.30 -0.8
1,611,085 + RR42_RS07535 0.30 +1.0
1,611,086 - RR42_RS07535 0.31 +0.8
1,611,121 + RR42_RS07535 0.35 -2.5
1,611,122 - RR42_RS07535 0.35 -1.5
1,611,176 + RR42_RS07535 0.41 -1.6
1,611,176 + RR42_RS07535 0.41 -1.5
1,611,176 + RR42_RS07535 0.41 -0.2
1,611,176 + RR42_RS07535 0.41 +1.3
1,611,176 + RR42_RS07535 0.41 -1.4
1,611,177 - RR42_RS07535 0.41 +1.4
1,611,177 - RR42_RS07535 0.41 +0.0
1,611,177 - RR42_RS07535 0.41 +0.6
1,611,239 - RR42_RS07535 0.48 +1.2
1,611,248 - RR42_RS07535 0.49 +0.8
1,611,295 + RR42_RS07535 0.55 -0.2
1,611,296 - RR42_RS07535 0.55 -1.0
1,611,296 - RR42_RS07535 0.55 -0.0
1,611,394 + RR42_RS07535 0.66 -0.1
1,611,583 - RR42_RS07535 0.88 +1.3
1,611,629 - +0.2
1,611,704 - +0.2
1,611,807 + RR42_RS07540 0.11 +0.2
1,611,808 - RR42_RS07540 0.11 +1.0
1,611,906 - RR42_RS07540 0.21 -1.0
1,611,938 + RR42_RS07540 0.24 +1.7
1,612,007 - RR42_RS07540 0.31 -0.8
1,612,085 + RR42_RS07540 0.38 +0.2
1,612,085 + RR42_RS07540 0.38 +0.6
1,612,099 + RR42_RS07540 0.40 -2.6
1,612,124 + RR42_RS07540 0.42 +0.3
1,612,124 + RR42_RS07540 0.42 -0.6
1,612,124 + RR42_RS07540 0.42 -0.3
1,612,124 - RR42_RS07540 0.42 +2.0
1,612,125 - RR42_RS07540 0.42 -0.5
1,612,125 - RR42_RS07540 0.42 -3.3
1,612,125 - RR42_RS07540 0.42 -0.0
1,612,172 + RR42_RS07540 0.47 +0.5
1,612,172 + RR42_RS07540 0.47 -0.1
1,612,172 + RR42_RS07540 0.47 +0.1
1,612,172 + RR42_RS07540 0.47 +0.7
1,612,172 + RR42_RS07540 0.47 +0.5
1,612,172 + RR42_RS07540 0.47 -0.3
1,612,172 + RR42_RS07540 0.47 -0.5
1,612,172 + RR42_RS07540 0.47 -0.5
1,612,173 - RR42_RS07540 0.47 -0.1
1,612,173 - RR42_RS07540 0.47 +0.3
1,612,173 - RR42_RS07540 0.47 -1.4
1,612,173 - RR42_RS07540 0.47 +0.8
1,612,173 - RR42_RS07540 0.47 -0.5
1,612,173 - RR42_RS07540 0.47 +0.6
1,612,173 - RR42_RS07540 0.47 -0.4
1,612,174 + RR42_RS07540 0.47 +3.9
1,612,175 - RR42_RS07540 0.47 +1.1
1,612,175 - RR42_RS07540 0.47 +0.5
1,612,175 - RR42_RS07540 0.47 +0.2
1,612,175 - RR42_RS07540 0.47 +0.1
1,612,175 - RR42_RS07540 0.47 +0.4
1,612,175 - RR42_RS07540 0.47 -0.2
1,612,179 - RR42_RS07540 0.47 +0.6
1,612,179 - RR42_RS07540 0.47 +0.4
1,612,244 + RR42_RS07540 0.54 -0.4
1,612,245 - RR42_RS07540 0.54 -0.1
1,612,294 - RR42_RS07540 0.59 +0.8
1,612,340 - RR42_RS07540 0.63 -0.0
1,612,376 + RR42_RS07540 0.67 +1.5
1,612,376 + RR42_RS07540 0.67 -1.4
1,612,376 + RR42_RS07540 0.67 -0.6
1,612,376 + RR42_RS07540 0.67 +0.0
1,612,376 + RR42_RS07540 0.67 -1.2
1,612,376 + RR42_RS07540 0.67 +0.8
1,612,377 - RR42_RS07540 0.67 -0.3
1,612,377 - RR42_RS07540 0.67 +0.9
1,612,434 - RR42_RS07540 0.72 -0.1
1,612,439 + RR42_RS07540 0.73 +0.7
1,612,439 + RR42_RS07540 0.73 -0.1
1,612,439 + RR42_RS07540 0.73 +0.0
1,612,440 - RR42_RS07540 0.73 +0.2
1,612,440 - RR42_RS07540 0.73 +2.3
1,612,881 + RR42_RS07545 0.20 -0.1
1,612,881 + RR42_RS07545 0.20 -0.9
1,612,881 + RR42_RS07545 0.20 -0.8
1,612,882 - RR42_RS07545 0.20 +0.4
1,612,882 - RR42_RS07545 0.20 -0.2
1,612,882 - RR42_RS07545 0.20 +0.2
1,612,882 - RR42_RS07545 0.20 +0.1
1,612,882 - RR42_RS07545 0.20 -0.8
1,612,882 - RR42_RS07545 0.20 +0.0
1,613,004 + RR42_RS07545 0.34 -3.8
1,613,005 - RR42_RS07545 0.34 -2.9
1,613,005 - RR42_RS07545 0.34 -0.0
1,613,465 - RR42_RS07545 0.88 +0.1
1,613,465 - RR42_RS07545 0.88 -0.5
1,613,545 - -0.4
1,613,607 + +0.8
1,613,608 - +0.0
1,613,608 - +1.0
1,613,711 + RR42_RS07550 0.21 +1.0
1,613,711 + RR42_RS07550 0.21 +2.2
1,613,712 - RR42_RS07550 0.21 -0.3

Or see this region's nucleotide sequence