Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07315 and RR42_RS07320 are separated by 32 nucleotides RR42_RS07320 and RR42_RS07325 are separated by 97 nucleotides RR42_RS07325 and RR42_RS07330 are separated by 180 nucleotides
RR42_RS07315: RR42_RS07315 - sulfate transporter, at 1,559,296 to 1,561,074
_RS07315
RR42_RS07320: RR42_RS07320 - MarR family transcriptional regulator, at 1,561,107 to 1,561,556
_RS07320
RR42_RS07325: RR42_RS07325 - exodeoxyribonuclease III, at 1,561,654 to 1,562,451
_RS07325
RR42_RS07330: RR42_RS07330 - histidine utilization repressor, at 1,562,632 to 1,563,345
_RS07330
Position (kb)
1561
1562
1563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1560.785 kb on + strand, within RR42_RS07315 at 1560.785 kb on + strand, within RR42_RS07315 at 1560.786 kb on - strand, within RR42_RS07315 at 1560.920 kb on + strand at 1560.921 kb on - strand at 1561.820 kb on + strand, within RR42_RS07325 at 1561.821 kb on - strand, within RR42_RS07325 at 1561.865 kb on + strand, within RR42_RS07325 at 1562.072 kb on + strand, within RR42_RS07325 at 1562.073 kb on - strand, within RR42_RS07325 at 1562.073 kb on - strand, within RR42_RS07325 at 1562.141 kb on + strand, within RR42_RS07325 at 1562.142 kb on - strand, within RR42_RS07325 at 1562.187 kb on + strand, within RR42_RS07325 at 1562.252 kb on + strand, within RR42_RS07325 at 1562.253 kb on - strand, within RR42_RS07325 at 1562.313 kb on - strand, within RR42_RS07325 at 1562.365 kb on - strand, within RR42_RS07325 at 1562.408 kb on - strand at 1562.452 kb on + strand at 1562.461 kb on - strand at 1562.468 kb on + strand at 1562.469 kb on - strand at 1562.511 kb on - strand at 1562.511 kb on - strand at 1562.531 kb on - strand at 1562.658 kb on + strand at 1562.658 kb on + strand at 1562.658 kb on + strand at 1562.658 kb on + strand at 1562.659 kb on - strand at 1562.659 kb on - strand at 1562.659 kb on - strand at 1562.807 kb on + strand, within RR42_RS07330 at 1562.807 kb on + strand, within RR42_RS07330 at 1562.843 kb on + strand, within RR42_RS07330 at 1562.844 kb on - strand, within RR42_RS07330 at 1562.844 kb on - strand, within RR42_RS07330 at 1562.844 kb on - strand, within RR42_RS07330 at 1562.882 kb on + strand, within RR42_RS07330 at 1562.882 kb on + strand, within RR42_RS07330 at 1562.909 kb on + strand, within RR42_RS07330 at 1562.910 kb on - strand, within RR42_RS07330 at 1562.946 kb on + strand, within RR42_RS07330 at 1563.299 kb on + strand at 1563.299 kb on + strand at 1563.299 kb on + strand at 1563.317 kb on + strand at 1563.338 kb on + strand at 1563.338 kb on + strand at 1563.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,560,785 + RR42_RS07315 0.84 -0.6 1,560,785 + RR42_RS07315 0.84 +0.3 1,560,786 - RR42_RS07315 0.84 -0.0 1,560,920 + -0.4 1,560,921 - -0.3 1,561,820 + RR42_RS07325 0.21 -2.0 1,561,821 - RR42_RS07325 0.21 -3.4 1,561,865 + RR42_RS07325 0.26 -1.6 1,562,072 + RR42_RS07325 0.52 -2.0 1,562,073 - RR42_RS07325 0.53 -1.7 1,562,073 - RR42_RS07325 0.53 -1.2 1,562,141 + RR42_RS07325 0.61 +0.0 1,562,142 - RR42_RS07325 0.61 -2.7 1,562,187 + RR42_RS07325 0.67 -1.3 1,562,252 + RR42_RS07325 0.75 -2.6 1,562,253 - RR42_RS07325 0.75 -2.4 1,562,313 - RR42_RS07325 0.83 -3.6 1,562,365 - RR42_RS07325 0.89 -4.4 1,562,408 - -3.0 1,562,452 + -0.1 1,562,461 - +0.1 1,562,468 + +0.5 1,562,469 - -0.1 1,562,511 - -0.4 1,562,511 - -2.2 1,562,531 - +0.5 1,562,658 + +0.1 1,562,658 + -0.4 1,562,658 + -2.0 1,562,658 + -0.9 1,562,659 - -1.6 1,562,659 - -0.2 1,562,659 - -3.2 1,562,807 + RR42_RS07330 0.25 -1.1 1,562,807 + RR42_RS07330 0.25 +0.4 1,562,843 + RR42_RS07330 0.30 -0.3 1,562,844 - RR42_RS07330 0.30 -2.1 1,562,844 - RR42_RS07330 0.30 -0.7 1,562,844 - RR42_RS07330 0.30 -1.3 1,562,882 + RR42_RS07330 0.35 -0.2 1,562,882 + RR42_RS07330 0.35 -2.3 1,562,909 + RR42_RS07330 0.39 -0.6 1,562,910 - RR42_RS07330 0.39 -0.9 1,562,946 + RR42_RS07330 0.44 -2.9 1,563,299 + -2.3 1,563,299 + -2.2 1,563,299 + -1.6 1,563,317 + -0.9 1,563,338 + -1.5 1,563,338 + -3.4 1,563,339 - -1.9
Or see this region's nucleotide sequence