Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07270 and RR42_RS07275 are separated by 347 nucleotides RR42_RS07275 and RR42_RS07280 are separated by 369 nucleotides
RR42_RS07270: RR42_RS07270 - NAD-glutamate dehydrogenase, at 1,546,204 to 1,551,084
_RS07270
RR42_RS07275: RR42_RS07275 - acetyl-CoA hydrolase, at 1,551,432 to 1,552,946
_RS07275
RR42_RS07280: RR42_RS07280 - hypothetical protein, at 1,553,316 to 1,553,699
_RS07280
Position (kb)
1551
1552
1553 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1550.847 kb on - strand at 1550.915 kb on + strand at 1550.966 kb on + strand at 1550.967 kb on - strand at 1550.969 kb on + strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1551.184 kb on + strand at 1551.184 kb on + strand at 1551.185 kb on - strand at 1551.188 kb on + strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.194 kb on - strand at 1551.194 kb on - strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.252 kb on + strand at 1551.253 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.435 kb on + strand at 1551.435 kb on + strand at 1551.436 kb on - strand at 1551.446 kb on + strand at 1551.447 kb on - strand at 1551.805 kb on + strand, within RR42_RS07275 at 1551.932 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.998 kb on + strand, within RR42_RS07275 at 1551.999 kb on - strand, within RR42_RS07275 at 1552.041 kb on - strand, within RR42_RS07275 at 1552.094 kb on + strand, within RR42_RS07275 at 1552.094 kb on + strand, within RR42_RS07275 at 1552.287 kb on + strand, within RR42_RS07275 at 1552.287 kb on + strand, within RR42_RS07275 at 1552.288 kb on - strand, within RR42_RS07275 at 1552.384 kb on - strand, within RR42_RS07275 at 1552.414 kb on - strand, within RR42_RS07275 at 1552.514 kb on + strand, within RR42_RS07275 at 1552.515 kb on - strand, within RR42_RS07275 at 1552.518 kb on + strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.797 kb on + strand at 1552.798 kb on - strand at 1552.798 kb on - strand at 1552.818 kb on + strand at 1552.818 kb on + strand at 1552.818 kb on + strand at 1552.819 kb on - strand at 1552.819 kb on - strand at 1552.850 kb on + strand at 1552.962 kb on + strand at 1552.962 kb on + strand at 1553.007 kb on + strand at 1553.008 kb on - strand at 1553.051 kb on + strand at 1553.051 kb on + strand at 1553.052 kb on - strand at 1553.052 kb on - strand at 1553.113 kb on - strand at 1553.137 kb on + strand at 1553.137 kb on + strand at 1553.137 kb on + strand at 1553.138 kb on - strand at 1553.138 kb on - strand at 1553.138 kb on - strand at 1553.343 kb on - strand at 1553.344 kb on + strand at 1553.344 kb on + strand at 1553.345 kb on - strand at 1553.345 kb on - strand at 1553.381 kb on - strand, within RR42_RS07280 at 1553.416 kb on + strand, within RR42_RS07280 at 1553.452 kb on + strand, within RR42_RS07280 at 1553.524 kb on + strand, within RR42_RS07280 at 1553.580 kb on - strand, within RR42_RS07280 at 1553.580 kb on - strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.615 kb on - strand, within RR42_RS07280 at 1553.615 kb on - strand, within RR42_RS07280 at 1553.716 kb on - strand at 1553.716 kb on - strand at 1553.794 kb on - strand at 1553.821 kb on - strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.839 kb on - strand at 1553.851 kb on - strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,550,847 - +1.7 1,550,915 + -0.1 1,550,966 + +0.2 1,550,967 - -0.2 1,550,969 + -3.5 1,550,970 - +1.1 1,550,970 - -1.3 1,550,970 - +0.4 1,551,184 + -0.2 1,551,184 + +1.2 1,551,185 - +0.5 1,551,188 + -3.0 1,551,189 - -1.0 1,551,189 - -1.2 1,551,189 - +0.7 1,551,193 + -0.2 1,551,193 + -0.0 1,551,193 + +1.3 1,551,193 + -0.7 1,551,194 - +1.3 1,551,194 - +0.5 1,551,200 + +1.1 1,551,200 + -2.2 1,551,200 + -1.1 1,551,252 + +1.2 1,551,253 - +0.8 1,551,314 - +1.3 1,551,314 - -2.0 1,551,314 - -1.5 1,551,428 + +0.6 1,551,428 + -0.8 1,551,428 + +0.8 1,551,428 + -2.8 1,551,428 + -0.1 1,551,428 + +1.2 1,551,428 + +0.3 1,551,428 + -1.3 1,551,429 - -0.1 1,551,429 - +0.8 1,551,429 - -1.5 1,551,429 - +0.2 1,551,429 - -1.7 1,551,435 + +1.3 1,551,435 + -0.4 1,551,436 - -2.6 1,551,446 + -0.3 1,551,447 - +0.8 1,551,805 + RR42_RS07275 0.25 +0.7 1,551,932 + RR42_RS07275 0.33 +1.0 1,551,966 + RR42_RS07275 0.35 -0.1 1,551,966 + RR42_RS07275 0.35 -0.2 1,551,966 + RR42_RS07275 0.35 +0.1 1,551,966 + RR42_RS07275 0.35 +0.1 1,551,967 - RR42_RS07275 0.35 +0.8 1,551,967 - RR42_RS07275 0.35 +1.0 1,551,998 + RR42_RS07275 0.37 +2.2 1,551,999 - RR42_RS07275 0.37 -1.1 1,552,041 - RR42_RS07275 0.40 -0.0 1,552,094 + RR42_RS07275 0.44 +1.8 1,552,094 + RR42_RS07275 0.44 -0.4 1,552,287 + RR42_RS07275 0.56 -0.7 1,552,287 + RR42_RS07275 0.56 +1.4 1,552,288 - RR42_RS07275 0.57 -3.5 1,552,384 - RR42_RS07275 0.63 -0.7 1,552,414 - RR42_RS07275 0.65 -1.4 1,552,514 + RR42_RS07275 0.71 -1.6 1,552,515 - RR42_RS07275 0.71 +0.1 1,552,518 + RR42_RS07275 0.72 +0.6 1,552,519 - RR42_RS07275 0.72 -0.3 1,552,519 - RR42_RS07275 0.72 -0.9 1,552,519 - RR42_RS07275 0.72 -0.5 1,552,519 - RR42_RS07275 0.72 -2.5 1,552,519 - RR42_RS07275 0.72 -0.3 1,552,797 + -1.7 1,552,798 - +0.1 1,552,798 - +0.1 1,552,818 + +0.4 1,552,818 + -2.5 1,552,818 + +0.3 1,552,819 - -0.0 1,552,819 - -1.6 1,552,850 + +3.2 1,552,962 + +0.6 1,552,962 + +0.7 1,553,007 + -0.3 1,553,008 - +0.4 1,553,051 + -1.0 1,553,051 + -0.5 1,553,052 - -1.1 1,553,052 - +0.6 1,553,113 - -1.7 1,553,137 + -1.7 1,553,137 + -1.6 1,553,137 + -1.2 1,553,138 - -0.0 1,553,138 - -0.1 1,553,138 - -1.2 1,553,343 - +0.4 1,553,344 + -2.3 1,553,344 + +0.4 1,553,345 - +1.3 1,553,345 - +1.5 1,553,381 - RR42_RS07280 0.17 +1.5 1,553,416 + RR42_RS07280 0.26 +1.7 1,553,452 + RR42_RS07280 0.35 -2.6 1,553,524 + RR42_RS07280 0.54 -0.4 1,553,580 - RR42_RS07280 0.69 -0.5 1,553,580 - RR42_RS07280 0.69 -0.2 1,553,614 + RR42_RS07280 0.78 -0.1 1,553,614 + RR42_RS07280 0.78 +0.1 1,553,614 + RR42_RS07280 0.78 -0.2 1,553,615 - RR42_RS07280 0.78 -0.8 1,553,615 - RR42_RS07280 0.78 +1.1 1,553,716 - -0.7 1,553,716 - +0.3 1,553,794 - -0.6 1,553,821 - -0.8 1,553,838 + +0.3 1,553,838 + -0.1 1,553,838 + +1.2 1,553,838 + -2.7 1,553,839 - -0.7 1,553,851 - -0.5 1,553,910 + -0.5 1,553,910 + -0.9 1,553,910 + +1.9 1,553,910 + -1.2 1,553,910 + +0.0 1,553,910 + +1.3 1,553,911 - -1.9
Or see this region's nucleotide sequence