Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07260 and RR42_RS07265 are separated by 207 nucleotides RR42_RS07265 and RR42_RS07270 are separated by 164 nucleotides RR42_RS07270 and RR42_RS07275 are separated by 347 nucleotides
RR42_RS07260: RR42_RS07260 - hypothetical protein, at 1,545,209 to 1,545,577
_RS07260
RR42_RS07265: RR42_RS07265 - hypothetical protein, at 1,545,785 to 1,546,039
_RS07265
RR42_RS07270: RR42_RS07270 - NAD-glutamate dehydrogenase, at 1,546,204 to 1,551,084
_RS07270
RR42_RS07275: RR42_RS07275 - acetyl-CoA hydrolase, at 1,551,432 to 1,552,946
_RS07275
Position (kb)
1546
1547
1548
1549
1550
1551
1552 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.408 kb on - strand, within RR42_RS07260 at 1545.408 kb on - strand, within RR42_RS07260 at 1545.420 kb on - strand, within RR42_RS07260 at 1545.575 kb on + strand at 1545.576 kb on - strand at 1545.598 kb on + strand at 1545.637 kb on + strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.679 kb on + strand at 1545.679 kb on + strand at 1545.680 kb on - strand at 1545.878 kb on + strand, within RR42_RS07265 at 1545.878 kb on + strand, within RR42_RS07265 at 1546.057 kb on + strand at 1546.057 kb on + strand at 1546.058 kb on - strand at 1546.107 kb on + strand at 1546.108 kb on - strand at 1546.202 kb on - strand at 1546.513 kb on - strand at 1546.513 kb on - strand at 1546.727 kb on - strand, within RR42_RS07270 at 1547.186 kb on + strand, within RR42_RS07270 at 1547.403 kb on - strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.405 kb on + strand, within RR42_RS07270 at 1547.406 kb on - strand, within RR42_RS07270 at 1547.417 kb on - strand, within RR42_RS07270 at 1547.521 kb on - strand, within RR42_RS07270 at 1547.741 kb on + strand, within RR42_RS07270 at 1547.741 kb on + strand, within RR42_RS07270 at 1547.742 kb on - strand, within RR42_RS07270 at 1547.742 kb on - strand, within RR42_RS07270 at 1547.761 kb on - strand, within RR42_RS07270 at 1547.762 kb on + strand, within RR42_RS07270 at 1547.763 kb on - strand, within RR42_RS07270 at 1547.763 kb on - strand, within RR42_RS07270 at 1548.012 kb on + strand, within RR42_RS07270 at 1548.012 kb on + strand, within RR42_RS07270 at 1548.045 kb on + strand, within RR42_RS07270 at 1548.045 kb on + strand, within RR42_RS07270 at 1548.210 kb on - strand, within RR42_RS07270 at 1548.326 kb on + strand, within RR42_RS07270 at 1548.327 kb on - strand, within RR42_RS07270 at 1548.562 kb on + strand, within RR42_RS07270 at 1548.563 kb on - strand, within RR42_RS07270 at 1548.770 kb on + strand, within RR42_RS07270 at 1548.770 kb on + strand, within RR42_RS07270 at 1548.958 kb on - strand, within RR42_RS07270 at 1548.989 kb on + strand, within RR42_RS07270 at 1548.989 kb on + strand, within RR42_RS07270 at 1548.990 kb on - strand, within RR42_RS07270 at 1548.990 kb on - strand, within RR42_RS07270 at 1548.990 kb on - strand, within RR42_RS07270 at 1549.026 kb on - strand, within RR42_RS07270 at 1549.035 kb on - strand, within RR42_RS07270 at 1549.472 kb on + strand, within RR42_RS07270 at 1549.509 kb on - strand, within RR42_RS07270 at 1549.517 kb on - strand, within RR42_RS07270 at 1549.775 kb on + strand, within RR42_RS07270 at 1549.775 kb on + strand, within RR42_RS07270 at 1549.775 kb on + strand, within RR42_RS07270 at 1549.775 kb on + strand, within RR42_RS07270 at 1549.776 kb on - strand, within RR42_RS07270 at 1549.964 kb on + strand, within RR42_RS07270 at 1549.965 kb on - strand, within RR42_RS07270 at 1550.054 kb on + strand, within RR42_RS07270 at 1550.054 kb on + strand, within RR42_RS07270 at 1550.054 kb on + strand, within RR42_RS07270 at 1550.054 kb on + strand, within RR42_RS07270 at 1550.055 kb on - strand, within RR42_RS07270 at 1550.055 kb on - strand, within RR42_RS07270 at 1550.055 kb on - strand, within RR42_RS07270 at 1550.069 kb on + strand, within RR42_RS07270 at 1550.069 kb on + strand, within RR42_RS07270 at 1550.070 kb on - strand, within RR42_RS07270 at 1550.109 kb on + strand, within RR42_RS07270 at 1550.110 kb on - strand, within RR42_RS07270 at 1550.136 kb on - strand, within RR42_RS07270 at 1550.315 kb on + strand, within RR42_RS07270 at 1550.316 kb on - strand, within RR42_RS07270 at 1550.345 kb on + strand, within RR42_RS07270 at 1550.346 kb on - strand, within RR42_RS07270 at 1550.847 kb on - strand at 1550.915 kb on + strand at 1550.966 kb on + strand at 1550.967 kb on - strand at 1550.969 kb on + strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1551.184 kb on + strand at 1551.184 kb on + strand at 1551.185 kb on - strand at 1551.188 kb on + strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.194 kb on - strand at 1551.194 kb on - strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.252 kb on + strand at 1551.253 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.435 kb on + strand at 1551.435 kb on + strand at 1551.436 kb on - strand at 1551.446 kb on + strand at 1551.447 kb on - strand at 1551.805 kb on + strand, within RR42_RS07275 at 1551.932 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.998 kb on + strand, within RR42_RS07275 at 1551.999 kb on - strand, within RR42_RS07275 at 1552.041 kb on - strand, within RR42_RS07275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,545,407 + RR42_RS07260 0.54 -1.7 1,545,407 + RR42_RS07260 0.54 -1.2 1,545,407 + RR42_RS07260 0.54 -1.3 1,545,407 + RR42_RS07260 0.54 -0.8 1,545,407 + RR42_RS07260 0.54 +1.2 1,545,408 - RR42_RS07260 0.54 +2.4 1,545,408 - RR42_RS07260 0.54 -2.2 1,545,420 - RR42_RS07260 0.57 -0.5 1,545,575 + +0.8 1,545,576 - +0.8 1,545,598 + -1.4 1,545,637 + -0.3 1,545,638 - -0.4 1,545,638 - +0.1 1,545,638 - -2.0 1,545,638 - -0.7 1,545,679 + -0.2 1,545,679 + -1.2 1,545,680 - -0.9 1,545,878 + RR42_RS07265 0.36 -0.8 1,545,878 + RR42_RS07265 0.36 -1.1 1,546,057 + -2.0 1,546,057 + -0.2 1,546,058 - -0.9 1,546,107 + -0.0 1,546,108 - +0.8 1,546,202 - -0.3 1,546,513 - -1.6 1,546,513 - -2.4 1,546,727 - RR42_RS07270 0.11 -0.4 1,547,186 + RR42_RS07270 0.20 +0.3 1,547,403 - RR42_RS07270 0.25 +2.0 1,547,405 + RR42_RS07270 0.25 +2.2 1,547,405 + RR42_RS07270 0.25 +0.6 1,547,405 + RR42_RS07270 0.25 +0.1 1,547,405 + RR42_RS07270 0.25 +1.0 1,547,405 + RR42_RS07270 0.25 -0.2 1,547,405 + RR42_RS07270 0.25 -3.2 1,547,406 - RR42_RS07270 0.25 -0.6 1,547,417 - RR42_RS07270 0.25 -2.1 1,547,521 - RR42_RS07270 0.27 -1.0 1,547,741 + RR42_RS07270 0.31 +0.3 1,547,741 + RR42_RS07270 0.31 -0.6 1,547,742 - RR42_RS07270 0.32 -0.2 1,547,742 - RR42_RS07270 0.32 -0.4 1,547,761 - RR42_RS07270 0.32 -2.1 1,547,762 + RR42_RS07270 0.32 -0.1 1,547,763 - RR42_RS07270 0.32 -0.2 1,547,763 - RR42_RS07270 0.32 -1.3 1,548,012 + RR42_RS07270 0.37 -0.3 1,548,012 + RR42_RS07270 0.37 -0.4 1,548,045 + RR42_RS07270 0.38 +1.5 1,548,045 + RR42_RS07270 0.38 -0.7 1,548,210 - RR42_RS07270 0.41 -0.7 1,548,326 + RR42_RS07270 0.43 +1.7 1,548,327 - RR42_RS07270 0.43 +0.6 1,548,562 + RR42_RS07270 0.48 -0.3 1,548,563 - RR42_RS07270 0.48 -0.3 1,548,770 + RR42_RS07270 0.53 -0.8 1,548,770 + RR42_RS07270 0.53 -0.6 1,548,958 - RR42_RS07270 0.56 +1.1 1,548,989 + RR42_RS07270 0.57 -0.3 1,548,989 + RR42_RS07270 0.57 +0.3 1,548,990 - RR42_RS07270 0.57 +1.2 1,548,990 - RR42_RS07270 0.57 -0.4 1,548,990 - RR42_RS07270 0.57 +1.2 1,549,026 - RR42_RS07270 0.58 -2.5 1,549,035 - RR42_RS07270 0.58 +1.8 1,549,472 + RR42_RS07270 0.67 +0.0 1,549,509 - RR42_RS07270 0.68 -0.2 1,549,517 - RR42_RS07270 0.68 +0.2 1,549,775 + RR42_RS07270 0.73 +0.2 1,549,775 + RR42_RS07270 0.73 -2.6 1,549,775 + RR42_RS07270 0.73 +0.1 1,549,775 + RR42_RS07270 0.73 +1.3 1,549,776 - RR42_RS07270 0.73 -0.5 1,549,964 + RR42_RS07270 0.77 +1.6 1,549,965 - RR42_RS07270 0.77 -0.2 1,550,054 + RR42_RS07270 0.79 +0.2 1,550,054 + RR42_RS07270 0.79 -0.6 1,550,054 + RR42_RS07270 0.79 -0.2 1,550,054 + RR42_RS07270 0.79 -0.8 1,550,055 - RR42_RS07270 0.79 -3.3 1,550,055 - RR42_RS07270 0.79 -0.4 1,550,055 - RR42_RS07270 0.79 +2.8 1,550,069 + RR42_RS07270 0.79 +0.1 1,550,069 + RR42_RS07270 0.79 +1.3 1,550,070 - RR42_RS07270 0.79 -1.1 1,550,109 + RR42_RS07270 0.80 -1.2 1,550,110 - RR42_RS07270 0.80 -1.7 1,550,136 - RR42_RS07270 0.81 -1.2 1,550,315 + RR42_RS07270 0.84 -1.0 1,550,316 - RR42_RS07270 0.84 +0.3 1,550,345 + RR42_RS07270 0.85 -1.2 1,550,346 - RR42_RS07270 0.85 -2.5 1,550,847 - +1.7 1,550,915 + -0.1 1,550,966 + +0.2 1,550,967 - -0.2 1,550,969 + -3.5 1,550,970 - +1.1 1,550,970 - -1.3 1,550,970 - +0.4 1,551,184 + -0.2 1,551,184 + +1.2 1,551,185 - +0.5 1,551,188 + -3.0 1,551,189 - -1.0 1,551,189 - -1.2 1,551,189 - +0.7 1,551,193 + -0.2 1,551,193 + -0.0 1,551,193 + +1.3 1,551,193 + -0.7 1,551,194 - +1.3 1,551,194 - +0.5 1,551,200 + +1.1 1,551,200 + -2.2 1,551,200 + -1.1 1,551,252 + +1.2 1,551,253 - +0.8 1,551,314 - +1.3 1,551,314 - -2.0 1,551,314 - -1.5 1,551,428 + +0.6 1,551,428 + -0.8 1,551,428 + +0.8 1,551,428 + -2.8 1,551,428 + -0.1 1,551,428 + +1.2 1,551,428 + +0.3 1,551,428 + -1.3 1,551,429 - -0.1 1,551,429 - +0.8 1,551,429 - -1.5 1,551,429 - +0.2 1,551,429 - -1.7 1,551,435 + +1.3 1,551,435 + -0.4 1,551,436 - -2.6 1,551,446 + -0.3 1,551,447 - +0.8 1,551,805 + RR42_RS07275 0.25 +0.7 1,551,932 + RR42_RS07275 0.33 +1.0 1,551,966 + RR42_RS07275 0.35 -0.1 1,551,966 + RR42_RS07275 0.35 -0.2 1,551,966 + RR42_RS07275 0.35 +0.1 1,551,966 + RR42_RS07275 0.35 +0.1 1,551,967 - RR42_RS07275 0.35 +0.8 1,551,967 - RR42_RS07275 0.35 +1.0 1,551,998 + RR42_RS07275 0.37 +2.2 1,551,999 - RR42_RS07275 0.37 -1.1 1,552,041 - RR42_RS07275 0.40 -0.0
Or see this region's nucleotide sequence