Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07255 and RR42_RS07260 are separated by 97 nucleotides RR42_RS07260 and RR42_RS07265 are separated by 207 nucleotides RR42_RS07265 and RR42_RS07270 are separated by 164 nucleotides
RR42_RS07255: RR42_RS07255 - hypothetical protein, at 1,544,860 to 1,545,111
_RS07255
RR42_RS07260: RR42_RS07260 - hypothetical protein, at 1,545,209 to 1,545,577
_RS07260
RR42_RS07265: RR42_RS07265 - hypothetical protein, at 1,545,785 to 1,546,039
_RS07265
RR42_RS07270: RR42_RS07270 - NAD-glutamate dehydrogenase, at 1,546,204 to 1,551,084
_RS07270
Position (kb)
1545
1546
1547 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.835 kb on - strand at 1544.835 kb on - strand at 1544.894 kb on - strand, within RR42_RS07255 at 1544.894 kb on - strand, within RR42_RS07255 at 1545.097 kb on + strand at 1545.134 kb on - strand at 1545.175 kb on + strand at 1545.175 kb on + strand at 1545.175 kb on + strand at 1545.175 kb on + strand at 1545.176 kb on - strand at 1545.176 kb on - strand at 1545.176 kb on - strand at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.407 kb on + strand, within RR42_RS07260 at 1545.408 kb on - strand, within RR42_RS07260 at 1545.408 kb on - strand, within RR42_RS07260 at 1545.420 kb on - strand, within RR42_RS07260 at 1545.575 kb on + strand at 1545.576 kb on - strand at 1545.598 kb on + strand at 1545.637 kb on + strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.638 kb on - strand at 1545.679 kb on + strand at 1545.679 kb on + strand at 1545.680 kb on - strand at 1545.878 kb on + strand, within RR42_RS07265 at 1545.878 kb on + strand, within RR42_RS07265 at 1546.057 kb on + strand at 1546.057 kb on + strand at 1546.058 kb on - strand at 1546.107 kb on + strand at 1546.108 kb on - strand at 1546.202 kb on - strand at 1546.513 kb on - strand at 1546.513 kb on - strand at 1546.727 kb on - strand, within RR42_RS07270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,544,834 + -0.2 1,544,834 + -0.2 1,544,834 + -1.1 1,544,834 + -0.3 1,544,834 + -0.1 1,544,835 - +0.3 1,544,835 - +0.0 1,544,894 - RR42_RS07255 0.13 +0.4 1,544,894 - RR42_RS07255 0.13 +0.8 1,545,097 + -1.9 1,545,134 - +0.4 1,545,175 + -0.2 1,545,175 + -0.9 1,545,175 + -1.2 1,545,175 + +1.8 1,545,176 - +0.9 1,545,176 - -1.0 1,545,176 - +0.4 1,545,407 + RR42_RS07260 0.54 -1.7 1,545,407 + RR42_RS07260 0.54 -1.2 1,545,407 + RR42_RS07260 0.54 -1.3 1,545,407 + RR42_RS07260 0.54 -0.8 1,545,407 + RR42_RS07260 0.54 +1.2 1,545,408 - RR42_RS07260 0.54 +2.4 1,545,408 - RR42_RS07260 0.54 -2.2 1,545,420 - RR42_RS07260 0.57 -0.5 1,545,575 + +0.8 1,545,576 - +0.8 1,545,598 + -1.4 1,545,637 + -0.3 1,545,638 - -0.4 1,545,638 - +0.1 1,545,638 - -2.0 1,545,638 - -0.7 1,545,679 + -0.2 1,545,679 + -1.2 1,545,680 - -0.9 1,545,878 + RR42_RS07265 0.36 -0.8 1,545,878 + RR42_RS07265 0.36 -1.1 1,546,057 + -2.0 1,546,057 + -0.2 1,546,058 - -0.9 1,546,107 + -0.0 1,546,108 - +0.8 1,546,202 - -0.3 1,546,513 - -1.6 1,546,513 - -2.4 1,546,727 - RR42_RS07270 0.11 -0.4
Or see this region's nucleotide sequence