Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07000 and RR42_RS07005 are separated by 291 nucleotides RR42_RS07005 and RR42_RS07010 are separated by 2 nucleotides RR42_RS07010 and RR42_RS07015 are separated by 3 nucleotides RR42_RS07015 and RR42_RS07020 are separated by 216 nucleotides
RR42_RS07000: RR42_RS07000 - hypothetical protein, at 1,504,578 to 1,504,781
_RS07000
RR42_RS07005: RR42_RS07005 - ATP synthase I, at 1,505,073 to 1,505,732
_RS07005
RR42_RS07010: RR42_RS07010 - hypothetical protein, at 1,505,735 to 1,506,070
_RS07010
RR42_RS07015: RR42_RS07015 - hypothetical protein, at 1,506,074 to 1,506,385
_RS07015
RR42_RS07020: RR42_RS07020 - hypothetical protein, at 1,506,602 to 1,507,156
_RS07020
Position (kb)
1505
1506
1507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.772 kb on - strand at 1504.772 kb on - strand at 1504.772 kb on - strand at 1504.782 kb on - strand at 1504.820 kb on + strand at 1504.820 kb on + strand at 1504.820 kb on + strand at 1504.821 kb on - strand at 1504.940 kb on - strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.075 kb on - strand at 1505.138 kb on - strand at 1505.208 kb on + strand, within RR42_RS07005 at 1505.208 kb on + strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.304 kb on + strand, within RR42_RS07005 at 1505.305 kb on - strand, within RR42_RS07005 at 1505.305 kb on - strand, within RR42_RS07005 at 1505.413 kb on + strand, within RR42_RS07005 at 1505.413 kb on + strand, within RR42_RS07005 at 1505.414 kb on - strand, within RR42_RS07005 at 1505.414 kb on - strand, within RR42_RS07005 at 1505.425 kb on + strand, within RR42_RS07005 at 1505.425 kb on + strand, within RR42_RS07005 at 1505.425 kb on + strand, within RR42_RS07005 at 1505.425 kb on + strand, within RR42_RS07005 at 1505.426 kb on - strand, within RR42_RS07005 at 1505.426 kb on - strand, within RR42_RS07005 at 1505.426 kb on - strand, within RR42_RS07005 at 1505.426 kb on - strand, within RR42_RS07005 at 1505.633 kb on - strand, within RR42_RS07005 at 1505.684 kb on - strand at 1505.719 kb on + strand at 1505.719 kb on + strand at 1505.719 kb on + strand at 1505.719 kb on + strand at 1505.719 kb on + strand at 1505.720 kb on - strand at 1505.720 kb on - strand at 1505.720 kb on - strand at 1505.720 kb on - strand at 1505.720 kb on - strand at 1505.723 kb on + strand at 1505.724 kb on - strand at 1505.724 kb on - strand at 1505.724 kb on - strand at 1505.736 kb on + strand at 1505.752 kb on + strand at 1505.753 kb on - strand at 1505.764 kb on - strand at 1505.895 kb on + strand, within RR42_RS07010 at 1505.896 kb on - strand, within RR42_RS07010 at 1505.896 kb on - strand, within RR42_RS07010 at 1505.896 kb on - strand, within RR42_RS07010 at 1505.991 kb on + strand, within RR42_RS07010 at 1505.992 kb on - strand, within RR42_RS07010 at 1505.992 kb on - strand, within RR42_RS07010 at 1506.027 kb on + strand, within RR42_RS07010 at 1506.028 kb on - strand, within RR42_RS07010 at 1506.033 kb on + strand, within RR42_RS07010 at 1506.034 kb on - strand, within RR42_RS07010 at 1506.211 kb on + strand, within RR42_RS07015 at 1506.212 kb on - strand, within RR42_RS07015 at 1506.212 kb on - strand, within RR42_RS07015 at 1506.212 kb on - strand, within RR42_RS07015 at 1506.322 kb on - strand, within RR42_RS07015 at 1506.322 kb on - strand, within RR42_RS07015 at 1506.347 kb on + strand, within RR42_RS07015 at 1506.375 kb on + strand at 1506.376 kb on - strand at 1506.376 kb on - strand at 1506.403 kb on - strand at 1506.623 kb on + strand at 1506.623 kb on + strand at 1506.844 kb on - strand, within RR42_RS07020 at 1506.935 kb on + strand, within RR42_RS07020 at 1506.992 kb on + strand, within RR42_RS07020 at 1507.004 kb on + strand, within RR42_RS07020 at 1507.005 kb on - strand, within RR42_RS07020 at 1507.022 kb on + strand, within RR42_RS07020 at 1507.022 kb on + strand, within RR42_RS07020 at 1507.023 kb on - strand, within RR42_RS07020 at 1507.023 kb on - strand, within RR42_RS07020 at 1507.040 kb on + strand, within RR42_RS07020 at 1507.070 kb on - strand, within RR42_RS07020 at 1507.070 kb on - strand, within RR42_RS07020 at 1507.070 kb on - strand, within RR42_RS07020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,504,771 + -3.7 1,504,771 + -1.8 1,504,771 + -1.0 1,504,771 + -1.1 1,504,772 - -0.6 1,504,772 - +0.2 1,504,772 - -0.5 1,504,782 - -0.6 1,504,820 + +1.6 1,504,820 + -2.5 1,504,820 + -0.5 1,504,821 - -1.3 1,504,940 - +0.1 1,505,074 + -2.7 1,505,074 + +0.5 1,505,074 + -0.4 1,505,074 + +0.5 1,505,075 - +0.3 1,505,138 - +0.1 1,505,208 + RR42_RS07005 0.20 -1.8 1,505,208 + RR42_RS07005 0.20 -1.7 1,505,209 - RR42_RS07005 0.21 +0.1 1,505,209 - RR42_RS07005 0.21 +0.3 1,505,209 - RR42_RS07005 0.21 -1.7 1,505,209 - RR42_RS07005 0.21 -0.3 1,505,304 + RR42_RS07005 0.35 +0.3 1,505,305 - RR42_RS07005 0.35 +1.6 1,505,305 - RR42_RS07005 0.35 +0.1 1,505,413 + RR42_RS07005 0.52 +0.1 1,505,413 + RR42_RS07005 0.52 -0.3 1,505,414 - RR42_RS07005 0.52 +0.1 1,505,414 - RR42_RS07005 0.52 -1.1 1,505,425 + RR42_RS07005 0.53 -1.6 1,505,425 + RR42_RS07005 0.53 -2.0 1,505,425 + RR42_RS07005 0.53 +0.4 1,505,425 + RR42_RS07005 0.53 -0.7 1,505,426 - RR42_RS07005 0.53 -1.3 1,505,426 - RR42_RS07005 0.53 +0.7 1,505,426 - RR42_RS07005 0.53 -0.8 1,505,426 - RR42_RS07005 0.53 +1.3 1,505,633 - RR42_RS07005 0.85 +1.1 1,505,684 - -0.2 1,505,719 + +0.4 1,505,719 + -1.4 1,505,719 + -2.3 1,505,719 + -1.6 1,505,719 + -2.0 1,505,720 - -0.3 1,505,720 - +1.3 1,505,720 - -0.4 1,505,720 - -2.0 1,505,720 - -1.2 1,505,723 + -0.6 1,505,724 - +0.4 1,505,724 - -0.3 1,505,724 - -1.0 1,505,736 + -0.7 1,505,752 + -0.4 1,505,753 - -1.9 1,505,764 - -0.6 1,505,895 + RR42_RS07010 0.48 +1.8 1,505,896 - RR42_RS07010 0.48 +0.9 1,505,896 - RR42_RS07010 0.48 +0.5 1,505,896 - RR42_RS07010 0.48 -0.7 1,505,991 + RR42_RS07010 0.76 -2.1 1,505,992 - RR42_RS07010 0.76 -0.7 1,505,992 - RR42_RS07010 0.76 +0.1 1,506,027 + RR42_RS07010 0.87 +1.4 1,506,028 - RR42_RS07010 0.87 -1.3 1,506,033 + RR42_RS07010 0.89 -2.3 1,506,034 - RR42_RS07010 0.89 -1.2 1,506,211 + RR42_RS07015 0.44 +1.0 1,506,212 - RR42_RS07015 0.44 -3.7 1,506,212 - RR42_RS07015 0.44 +0.3 1,506,212 - RR42_RS07015 0.44 +0.4 1,506,322 - RR42_RS07015 0.79 -1.7 1,506,322 - RR42_RS07015 0.79 +0.7 1,506,347 + RR42_RS07015 0.88 +1.2 1,506,375 + +0.5 1,506,376 - -0.0 1,506,376 - -1.0 1,506,403 - -0.4 1,506,623 + -0.4 1,506,623 + -0.5 1,506,844 - RR42_RS07020 0.44 -2.7 1,506,935 + RR42_RS07020 0.60 +0.3 1,506,992 + RR42_RS07020 0.70 -1.8 1,507,004 + RR42_RS07020 0.72 +1.2 1,507,005 - RR42_RS07020 0.73 +1.1 1,507,022 + RR42_RS07020 0.76 +0.6 1,507,022 + RR42_RS07020 0.76 -0.0 1,507,023 - RR42_RS07020 0.76 -0.7 1,507,023 - RR42_RS07020 0.76 -0.5 1,507,040 + RR42_RS07020 0.79 -1.5 1,507,070 - RR42_RS07020 0.84 -1.5 1,507,070 - RR42_RS07020 0.84 +0.2 1,507,070 - RR42_RS07020 0.84 -1.8
Or see this region's nucleotide sequence