Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06705 and RR42_RS06710 overlap by 4 nucleotides RR42_RS06710 and RR42_RS06715 overlap by 4 nucleotides RR42_RS06715 and RR42_RS06720 overlap by 4 nucleotides RR42_RS06720 and RR42_RS06725 are separated by 422 nucleotides
RR42_RS06705: RR42_RS06705 - hypothetical protein, at 1,458,600 to 1,458,821
_RS06705
RR42_RS06710: RR42_RS06710 - hypothetical protein, at 1,458,818 to 1,459,051
_RS06710
RR42_RS06715: RR42_RS06715 - hypothetical protein, at 1,459,048 to 1,460,220
_RS06715
RR42_RS06720: RR42_RS06720 - hypothetical protein, at 1,460,217 to 1,460,483
_RS06720
RR42_RS06725: RR42_RS06725 - hypothetical protein, at 1,460,906 to 1,461,565
_RS06725
Position (kb)
1459
1460
1461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1458.078 kb on + strand at 1458.079 kb on - strand at 1458.110 kb on - strand at 1458.481 kb on + strand at 1458.481 kb on + strand at 1458.482 kb on - strand at 1458.482 kb on - strand at 1458.586 kb on + strand at 1458.587 kb on - strand at 1458.601 kb on + strand at 1458.606 kb on + strand at 1458.606 kb on + strand at 1458.606 kb on + strand at 1458.606 kb on + strand at 1458.606 kb on + strand at 1458.607 kb on - strand at 1458.607 kb on - strand at 1458.752 kb on + strand, within RR42_RS06705 at 1458.821 kb on + strand at 1458.822 kb on - strand at 1458.828 kb on + strand at 1458.828 kb on + strand at 1458.829 kb on - strand at 1458.829 kb on - strand at 1458.829 kb on - strand at 1458.829 kb on - strand at 1458.829 kb on - strand at 1458.829 kb on - strand at 1458.832 kb on + strand at 1458.955 kb on - strand, within RR42_RS06710 at 1459.179 kb on - strand, within RR42_RS06715 at 1459.188 kb on + strand, within RR42_RS06715 at 1459.189 kb on - strand, within RR42_RS06715 at 1459.270 kb on - strand, within RR42_RS06715 at 1459.459 kb on - strand, within RR42_RS06715 at 1459.533 kb on - strand, within RR42_RS06715 at 1459.533 kb on - strand, within RR42_RS06715 at 1459.533 kb on - strand, within RR42_RS06715 at 1459.632 kb on + strand, within RR42_RS06715 at 1459.632 kb on + strand, within RR42_RS06715 at 1459.655 kb on - strand, within RR42_RS06715 at 1459.655 kb on - strand, within RR42_RS06715 at 1459.655 kb on - strand, within RR42_RS06715 at 1460.062 kb on - strand, within RR42_RS06715 at 1460.062 kb on - strand, within RR42_RS06715 at 1460.115 kb on + strand at 1460.116 kb on - strand at 1460.119 kb on + strand at 1460.119 kb on + strand at 1460.119 kb on + strand at 1460.129 kb on + strand at 1460.271 kb on - strand, within RR42_RS06720 at 1460.291 kb on - strand, within RR42_RS06720 at 1460.357 kb on - strand, within RR42_RS06720 at 1460.403 kb on + strand, within RR42_RS06720 at 1460.403 kb on + strand, within RR42_RS06720 at 1460.403 kb on + strand, within RR42_RS06720 at 1460.404 kb on - strand, within RR42_RS06720 at 1460.477 kb on + strand at 1460.780 kb on - strand at 1460.806 kb on + strand at 1460.883 kb on + strand at 1460.883 kb on + strand at 1460.884 kb on - strand at 1460.884 kb on - strand at 1460.884 kb on - strand at 1461.001 kb on - strand, within RR42_RS06725 at 1461.001 kb on - strand, within RR42_RS06725 at 1461.083 kb on - strand, within RR42_RS06725 at 1461.196 kb on - strand, within RR42_RS06725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,458,078 + -1.0 1,458,079 - -2.1 1,458,110 - -1.5 1,458,481 + -0.7 1,458,481 + +0.4 1,458,482 - -1.0 1,458,482 - -1.9 1,458,586 + -0.2 1,458,587 - -0.0 1,458,601 + +0.5 1,458,606 + +0.2 1,458,606 + -2.3 1,458,606 + +0.5 1,458,606 + -0.2 1,458,606 + +0.3 1,458,607 - -0.6 1,458,607 - +1.7 1,458,752 + RR42_RS06705 0.68 -0.1 1,458,821 + -0.3 1,458,822 - -0.3 1,458,828 + -1.1 1,458,828 + -0.5 1,458,829 - +2.1 1,458,829 - +0.0 1,458,829 - -1.6 1,458,829 - -1.5 1,458,829 - -2.6 1,458,829 - +0.2 1,458,832 + +1.0 1,458,955 - RR42_RS06710 0.59 +0.6 1,459,179 - RR42_RS06715 0.11 -1.1 1,459,188 + RR42_RS06715 0.12 +0.2 1,459,189 - RR42_RS06715 0.12 -0.8 1,459,270 - RR42_RS06715 0.19 -3.0 1,459,459 - RR42_RS06715 0.35 -1.1 1,459,533 - RR42_RS06715 0.41 +0.9 1,459,533 - RR42_RS06715 0.41 -0.6 1,459,533 - RR42_RS06715 0.41 -0.6 1,459,632 + RR42_RS06715 0.50 +0.1 1,459,632 + RR42_RS06715 0.50 -0.1 1,459,655 - RR42_RS06715 0.52 -0.8 1,459,655 - RR42_RS06715 0.52 -0.4 1,459,655 - RR42_RS06715 0.52 -0.8 1,460,062 - RR42_RS06715 0.86 -0.1 1,460,062 - RR42_RS06715 0.86 +0.1 1,460,115 + +0.9 1,460,116 - -0.1 1,460,119 + +0.8 1,460,119 + +0.4 1,460,119 + +0.3 1,460,129 + -0.4 1,460,271 - RR42_RS06720 0.20 -0.2 1,460,291 - RR42_RS06720 0.28 +0.0 1,460,357 - RR42_RS06720 0.52 -1.5 1,460,403 + RR42_RS06720 0.70 +0.6 1,460,403 + RR42_RS06720 0.70 -0.3 1,460,403 + RR42_RS06720 0.70 +1.0 1,460,404 - RR42_RS06720 0.70 -0.6 1,460,477 + +1.1 1,460,780 - -0.7 1,460,806 + -1.9 1,460,883 + +0.3 1,460,883 + +0.1 1,460,884 - +0.9 1,460,884 - -0.7 1,460,884 - -1.5 1,461,001 - RR42_RS06725 0.14 +0.8 1,461,001 - RR42_RS06725 0.14 +0.1 1,461,083 - RR42_RS06725 0.27 -1.8 1,461,196 - RR42_RS06725 0.44 +0.4
Or see this region's nucleotide sequence