Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06675 and RR42_RS06680 are separated by 60 nucleotides RR42_RS06680 and RR42_RS06685 are separated by 108 nucleotides RR42_RS06685 and RR42_RS06690 overlap by 4 nucleotides RR42_RS06690 and RR42_RS06695 overlap by 4 nucleotides
RR42_RS06675: RR42_RS06675 - hypothetical protein, at 1,453,586 to 1,454,197
_RS06675
RR42_RS06680: RR42_RS06680 - hypothetical protein, at 1,454,258 to 1,454,866
_RS06680
RR42_RS06685: RR42_RS06685 - hypothetical protein, at 1,454,975 to 1,455,172
_RS06685
RR42_RS06690: RR42_RS06690 - hypothetical protein, at 1,455,169 to 1,455,624
_RS06690
RR42_RS06695: RR42_RS06695 - hypothetical protein, at 1,455,621 to 1,456,721
_RS06695
Position (kb)
1455
1456 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1454.218 kb on - strand at 1454.221 kb on + strand at 1454.222 kb on - strand at 1454.222 kb on - strand at 1454.392 kb on - strand, within RR42_RS06680 at 1454.467 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.469 kb on + strand, within RR42_RS06680 at 1454.475 kb on + strand, within RR42_RS06680 at 1454.553 kb on + strand, within RR42_RS06680 at 1454.586 kb on + strand, within RR42_RS06680 at 1454.587 kb on - strand, within RR42_RS06680 at 1454.750 kb on - strand, within RR42_RS06680 at 1454.776 kb on - strand, within RR42_RS06680 at 1454.930 kb on + strand at 1454.930 kb on + strand at 1454.931 kb on - strand at 1455.309 kb on + strand, within RR42_RS06690 at 1455.383 kb on + strand, within RR42_RS06690 at 1455.384 kb on - strand, within RR42_RS06690 at 1455.523 kb on + strand, within RR42_RS06690 at 1455.575 kb on + strand, within RR42_RS06690 at 1455.576 kb on - strand, within RR42_RS06690 at 1455.576 kb on - strand, within RR42_RS06690 at 1455.603 kb on - strand at 1455.603 kb on - strand at 1455.605 kb on + strand at 1455.655 kb on - strand at 1455.938 kb on - strand, within RR42_RS06695 at 1455.938 kb on - strand, within RR42_RS06695 at 1455.939 kb on + strand, within RR42_RS06695 at 1455.939 kb on + strand, within RR42_RS06695 at 1455.940 kb on - strand, within RR42_RS06695 at 1456.010 kb on - strand, within RR42_RS06695 at 1456.148 kb on - strand, within RR42_RS06695 at 1456.165 kb on + strand, within RR42_RS06695 at 1456.165 kb on + strand, within RR42_RS06695 at 1456.166 kb on - strand, within RR42_RS06695 at 1456.264 kb on + strand, within RR42_RS06695 at 1456.312 kb on + strand, within RR42_RS06695 at 1456.313 kb on - strand, within RR42_RS06695 at 1456.342 kb on + strand, within RR42_RS06695 at 1456.432 kb on - strand, within RR42_RS06695 at 1456.452 kb on - strand, within RR42_RS06695 at 1456.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,454,218 - -0.3 1,454,221 + +0.4 1,454,222 - -1.1 1,454,222 - +0.2 1,454,392 - RR42_RS06680 0.22 -1.3 1,454,467 + RR42_RS06680 0.34 -0.7 1,454,469 + RR42_RS06680 0.35 -1.5 1,454,469 + RR42_RS06680 0.35 -4.2 1,454,469 + RR42_RS06680 0.35 +0.9 1,454,469 + RR42_RS06680 0.35 -0.6 1,454,475 + RR42_RS06680 0.36 -0.2 1,454,553 + RR42_RS06680 0.48 -0.4 1,454,586 + RR42_RS06680 0.54 +1.5 1,454,587 - RR42_RS06680 0.54 -0.8 1,454,750 - RR42_RS06680 0.81 -2.1 1,454,776 - RR42_RS06680 0.85 -1.9 1,454,930 + -0.2 1,454,930 + -0.3 1,454,931 - -1.5 1,455,309 + RR42_RS06690 0.31 -0.1 1,455,383 + RR42_RS06690 0.47 +1.1 1,455,384 - RR42_RS06690 0.47 -1.7 1,455,523 + RR42_RS06690 0.78 -0.3 1,455,575 + RR42_RS06690 0.89 +0.9 1,455,576 - RR42_RS06690 0.89 +1.5 1,455,576 - RR42_RS06690 0.89 +0.7 1,455,603 - -0.9 1,455,603 - -0.8 1,455,605 + +1.5 1,455,655 - -0.8 1,455,938 - RR42_RS06695 0.29 +1.3 1,455,938 - RR42_RS06695 0.29 +0.5 1,455,939 + RR42_RS06695 0.29 +0.4 1,455,939 + RR42_RS06695 0.29 +0.9 1,455,940 - RR42_RS06695 0.29 -3.3 1,456,010 - RR42_RS06695 0.35 +1.3 1,456,148 - RR42_RS06695 0.48 +0.1 1,456,165 + RR42_RS06695 0.49 +1.5 1,456,165 + RR42_RS06695 0.49 -0.2 1,456,166 - RR42_RS06695 0.50 -3.4 1,456,264 + RR42_RS06695 0.58 -1.7 1,456,312 + RR42_RS06695 0.63 +0.5 1,456,313 - RR42_RS06695 0.63 -2.6 1,456,342 + RR42_RS06695 0.65 -2.6 1,456,432 - RR42_RS06695 0.74 -1.0 1,456,452 - RR42_RS06695 0.75 +0.1 1,456,619 - -2.3
Or see this region's nucleotide sequence