Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06675

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06665 and RR42_RS06670 are separated by 220 nucleotidesRR42_RS06670 and RR42_RS06675 are separated by 17 nucleotidesRR42_RS06675 and RR42_RS06680 are separated by 60 nucleotidesRR42_RS06680 and RR42_RS06685 are separated by 108 nucleotidesRR42_RS06685 and RR42_RS06690 overlap by 4 nucleotides RR42_RS06665: RR42_RS06665 - reverse transcriptase, at 1,451,819 to 1,452,994 _RS06665 RR42_RS06670: RR42_RS06670 - hypothetical protein, at 1,453,215 to 1,453,568 _RS06670 RR42_RS06675: RR42_RS06675 - hypothetical protein, at 1,453,586 to 1,454,197 _RS06675 RR42_RS06680: RR42_RS06680 - hypothetical protein, at 1,454,258 to 1,454,866 _RS06680 RR42_RS06685: RR42_RS06685 - hypothetical protein, at 1,454,975 to 1,455,172 _RS06685 RR42_RS06690: RR42_RS06690 - hypothetical protein, at 1,455,169 to 1,455,624 _RS06690 Position (kb) 1453 1454 1455Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1452.631 kb on + strand, within RR42_RS06665at 1452.632 kb on - strand, within RR42_RS06665at 1452.651 kb on + strand, within RR42_RS06665at 1452.651 kb on + strand, within RR42_RS06665at 1452.652 kb on - strand, within RR42_RS06665at 1452.652 kb on - strand, within RR42_RS06665at 1452.675 kb on + strand, within RR42_RS06665at 1452.675 kb on + strand, within RR42_RS06665at 1452.675 kb on + strand, within RR42_RS06665at 1452.675 kb on + strand, within RR42_RS06665at 1452.676 kb on - strand, within RR42_RS06665at 1452.676 kb on - strand, within RR42_RS06665at 1452.680 kb on + strand, within RR42_RS06665at 1452.681 kb on - strand, within RR42_RS06665at 1452.681 kb on - strand, within RR42_RS06665at 1452.719 kb on + strand, within RR42_RS06665at 1452.809 kb on + strand, within RR42_RS06665at 1452.810 kb on - strand, within RR42_RS06665at 1452.821 kb on + strand, within RR42_RS06665at 1452.883 kb on - strandat 1452.966 kb on - strandat 1453.046 kb on - strandat 1453.284 kb on + strand, within RR42_RS06670at 1453.490 kb on + strand, within RR42_RS06670at 1453.490 kb on + strand, within RR42_RS06670at 1453.528 kb on + strand, within RR42_RS06670at 1453.528 kb on + strand, within RR42_RS06670at 1453.541 kb on - strandat 1453.580 kb on - strandat 1453.583 kb on + strandat 1453.621 kb on - strandat 1453.752 kb on + strand, within RR42_RS06675at 1453.752 kb on + strand, within RR42_RS06675at 1453.785 kb on + strand, within RR42_RS06675at 1454.019 kb on + strand, within RR42_RS06675at 1454.019 kb on + strand, within RR42_RS06675at 1454.042 kb on + strand, within RR42_RS06675at 1454.043 kb on - strand, within RR42_RS06675at 1454.043 kb on - strand, within RR42_RS06675at 1454.043 kb on - strand, within RR42_RS06675at 1454.100 kb on - strand, within RR42_RS06675at 1454.218 kb on - strandat 1454.221 kb on + strandat 1454.222 kb on - strandat 1454.222 kb on - strandat 1454.392 kb on - strand, within RR42_RS06680at 1454.467 kb on + strand, within RR42_RS06680at 1454.469 kb on + strand, within RR42_RS06680at 1454.469 kb on + strand, within RR42_RS06680at 1454.469 kb on + strand, within RR42_RS06680at 1454.469 kb on + strand, within RR42_RS06680at 1454.475 kb on + strand, within RR42_RS06680at 1454.553 kb on + strand, within RR42_RS06680at 1454.586 kb on + strand, within RR42_RS06680at 1454.587 kb on - strand, within RR42_RS06680at 1454.750 kb on - strand, within RR42_RS06680at 1454.776 kb on - strand, within RR42_RS06680at 1454.930 kb on + strandat 1454.930 kb on + strandat 1454.931 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,452,631 + RR42_RS06665 0.69 +2.1
1,452,632 - RR42_RS06665 0.69 -1.3
1,452,651 + RR42_RS06665 0.71 -0.4
1,452,651 + RR42_RS06665 0.71 +0.0
1,452,652 - RR42_RS06665 0.71 +0.3
1,452,652 - RR42_RS06665 0.71 -0.1
1,452,675 + RR42_RS06665 0.73 -1.1
1,452,675 + RR42_RS06665 0.73 -1.4
1,452,675 + RR42_RS06665 0.73 +1.2
1,452,675 + RR42_RS06665 0.73 +1.9
1,452,676 - RR42_RS06665 0.73 +1.2
1,452,676 - RR42_RS06665 0.73 -0.9
1,452,680 + RR42_RS06665 0.73 +0.4
1,452,681 - RR42_RS06665 0.73 -3.1
1,452,681 - RR42_RS06665 0.73 -0.4
1,452,719 + RR42_RS06665 0.77 -1.1
1,452,809 + RR42_RS06665 0.84 -1.7
1,452,810 - RR42_RS06665 0.84 -0.9
1,452,821 + RR42_RS06665 0.85 -1.7
1,452,883 - +0.3
1,452,966 - -1.7
1,453,046 - -0.4
1,453,284 + RR42_RS06670 0.19 -0.0
1,453,490 + RR42_RS06670 0.78 +1.0
1,453,490 + RR42_RS06670 0.78 -1.7
1,453,528 + RR42_RS06670 0.88 +0.5
1,453,528 + RR42_RS06670 0.88 -1.7
1,453,541 - +0.9
1,453,580 - -2.2
1,453,583 + +0.3
1,453,621 - -0.3
1,453,752 + RR42_RS06675 0.27 -2.1
1,453,752 + RR42_RS06675 0.27 -1.1
1,453,785 + RR42_RS06675 0.33 +1.5
1,454,019 + RR42_RS06675 0.71 +0.7
1,454,019 + RR42_RS06675 0.71 -2.3
1,454,042 + RR42_RS06675 0.75 +0.2
1,454,043 - RR42_RS06675 0.75 -3.5
1,454,043 - RR42_RS06675 0.75 +0.3
1,454,043 - RR42_RS06675 0.75 -1.2
1,454,100 - RR42_RS06675 0.84 -1.6
1,454,218 - -0.3
1,454,221 + +0.4
1,454,222 - -1.1
1,454,222 - +0.2
1,454,392 - RR42_RS06680 0.22 -1.3
1,454,467 + RR42_RS06680 0.34 -0.7
1,454,469 + RR42_RS06680 0.35 -1.5
1,454,469 + RR42_RS06680 0.35 -4.2
1,454,469 + RR42_RS06680 0.35 +0.9
1,454,469 + RR42_RS06680 0.35 -0.6
1,454,475 + RR42_RS06680 0.36 -0.2
1,454,553 + RR42_RS06680 0.48 -0.4
1,454,586 + RR42_RS06680 0.54 +1.5
1,454,587 - RR42_RS06680 0.54 -0.8
1,454,750 - RR42_RS06680 0.81 -2.1
1,454,776 - RR42_RS06680 0.85 -1.9
1,454,930 + -0.2
1,454,930 + -0.3
1,454,931 - -1.5

Or see this region's nucleotide sequence