Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06520 and RR42_RS06525 are separated by 367 nucleotides RR42_RS06525 and RR42_RS06530 overlap by 4 nucleotides RR42_RS06530 and RR42_RS06535 are separated by 69 nucleotides
RR42_RS06520: RR42_RS06520 - short-chain dehydrogenase, at 1,427,288 to 1,428,073
_RS06520
RR42_RS06525: RR42_RS06525 - C4-dicarboxylate ABC transporter permease, at 1,428,441 to 1,429,049
_RS06525
RR42_RS06530: RR42_RS06530 - C4-dicarboxylate ABC transporter, at 1,429,046 to 1,430,350
_RS06530
RR42_RS06535: RR42_RS06535 - C4-dicarboxylate ABC transporter, at 1,430,420 to 1,431,448
_RS06535
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1428.071 kb on + strand at 1428.176 kb on + strand at 1428.344 kb on - strand at 1428.344 kb on - strand at 1428.544 kb on + strand, within RR42_RS06525 at 1428.545 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.913 kb on - strand, within RR42_RS06525 at 1428.967 kb on + strand, within RR42_RS06525 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.657 kb on + strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.808 kb on + strand, within RR42_RS06530 at 1429.809 kb on - strand, within RR42_RS06530 at 1429.836 kb on - strand, within RR42_RS06530 at 1430.336 kb on + strand at 1430.336 kb on + strand at 1430.337 kb on - strand at 1430.362 kb on + strand at 1430.383 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.456 kb on - strand at 1430.504 kb on + strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.777 kb on + strand, within RR42_RS06535 at 1430.777 kb on + strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.966 kb on + strand, within RR42_RS06535 at 1430.967 kb on - strand, within RR42_RS06535 at 1430.968 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.099 kb on - strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.299 kb on - strand, within RR42_RS06535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,428,071 + +0.1 1,428,176 + -1.7 1,428,344 - -0.2 1,428,344 - +0.5 1,428,544 + RR42_RS06525 0.17 -0.1 1,428,545 - RR42_RS06525 0.17 -0.5 1,428,628 - RR42_RS06525 0.31 -1.5 1,428,628 - RR42_RS06525 0.31 -2.4 1,428,864 + RR42_RS06525 0.69 -2.8 1,428,864 + RR42_RS06525 0.69 -0.6 1,428,865 - RR42_RS06525 0.70 +0.9 1,428,865 - RR42_RS06525 0.70 -0.4 1,428,865 - RR42_RS06525 0.70 -1.2 1,428,865 - RR42_RS06525 0.70 +0.0 1,428,913 - RR42_RS06525 0.78 -1.7 1,428,967 + RR42_RS06525 0.86 -0.9 1,429,223 + RR42_RS06530 0.14 -0.5 1,429,223 + RR42_RS06530 0.14 -1.9 1,429,224 - RR42_RS06530 0.14 +0.1 1,429,224 - RR42_RS06530 0.14 -1.4 1,429,224 - RR42_RS06530 0.14 -1.6 1,429,224 - RR42_RS06530 0.14 -0.3 1,429,538 + RR42_RS06530 0.38 +0.4 1,429,538 + RR42_RS06530 0.38 +0.1 1,429,538 + RR42_RS06530 0.38 +0.2 1,429,539 - RR42_RS06530 0.38 -0.1 1,429,539 - RR42_RS06530 0.38 +0.3 1,429,539 - RR42_RS06530 0.38 +0.5 1,429,539 - RR42_RS06530 0.38 +1.6 1,429,540 + RR42_RS06530 0.38 -0.8 1,429,540 + RR42_RS06530 0.38 +0.4 1,429,540 + RR42_RS06530 0.38 -0.1 1,429,657 + RR42_RS06530 0.47 -1.0 1,429,658 - RR42_RS06530 0.47 +0.4 1,429,658 - RR42_RS06530 0.47 -0.1 1,429,658 - RR42_RS06530 0.47 +1.4 1,429,808 + RR42_RS06530 0.58 +1.5 1,429,809 - RR42_RS06530 0.58 -1.0 1,429,836 - RR42_RS06530 0.61 -0.1 1,430,336 + -0.1 1,430,336 + -0.7 1,430,337 - +1.8 1,430,362 + -0.4 1,430,383 + +1.5 1,430,455 + +2.9 1,430,455 + -0.1 1,430,455 + +2.5 1,430,456 - -0.2 1,430,504 + -0.1 1,430,505 - +1.5 1,430,505 - +1.0 1,430,505 - +1.3 1,430,777 + RR42_RS06535 0.35 +0.7 1,430,777 + RR42_RS06535 0.35 -0.1 1,430,778 - RR42_RS06535 0.35 -0.8 1,430,778 - RR42_RS06535 0.35 +0.2 1,430,778 - RR42_RS06535 0.35 -1.0 1,430,966 + RR42_RS06535 0.53 +0.0 1,430,967 - RR42_RS06535 0.53 -1.7 1,430,968 + RR42_RS06535 0.53 +0.5 1,431,098 + RR42_RS06535 0.66 -0.6 1,431,098 + RR42_RS06535 0.66 -1.5 1,431,099 - RR42_RS06535 0.66 +0.6 1,431,116 + RR42_RS06535 0.68 -1.1 1,431,116 + RR42_RS06535 0.68 -0.4 1,431,117 - RR42_RS06535 0.68 -0.6 1,431,117 - RR42_RS06535 0.68 -0.2 1,431,134 + RR42_RS06535 0.69 +1.4 1,431,134 + RR42_RS06535 0.69 -1.1 1,431,135 - RR42_RS06535 0.69 -0.7 1,431,135 - RR42_RS06535 0.69 +0.2 1,431,135 - RR42_RS06535 0.69 -0.4 1,431,299 - RR42_RS06535 0.85 +0.5
Or see this region's nucleotide sequence