Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06300

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06295 and RR42_RS06300 are separated by 165 nucleotidesRR42_RS06300 and RR42_RS06305 are separated by 155 nucleotidesRR42_RS06305 and RR42_RS06310 are separated by 154 nucleotides RR42_RS06295: RR42_RS06295 - amino acid transporter, at 1,381,131 to 1,383,098 _RS06295 RR42_RS06300: RR42_RS06300 - DNA-binding protein, at 1,383,264 to 1,384,739 _RS06300 RR42_RS06305: RR42_RS06305 - hypothetical protein, at 1,384,895 to 1,385,530 _RS06305 RR42_RS06310: RR42_RS06310 - inositol monophosphatase, at 1,385,685 to 1,386,506 _RS06310 Position (kb) 1383 1384 1385Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1382.264 kb on - strand, within RR42_RS06295at 1382.312 kb on - strand, within RR42_RS06295at 1382.312 kb on - strand, within RR42_RS06295at 1382.312 kb on - strand, within RR42_RS06295at 1382.312 kb on - strand, within RR42_RS06295at 1382.392 kb on + strand, within RR42_RS06295at 1382.392 kb on + strand, within RR42_RS06295at 1382.393 kb on - strand, within RR42_RS06295at 1382.397 kb on - strand, within RR42_RS06295at 1382.679 kb on + strand, within RR42_RS06295at 1382.854 kb on + strand, within RR42_RS06295at 1382.874 kb on + strand, within RR42_RS06295at 1382.874 kb on + strand, within RR42_RS06295at 1382.875 kb on - strand, within RR42_RS06295at 1382.918 kb on + strandat 1382.919 kb on - strandat 1382.957 kb on - strandat 1383.214 kb on + strandat 1383.214 kb on + strandat 1383.214 kb on + strandat 1383.214 kb on + strandat 1383.215 kb on - strandat 1383.215 kb on - strandat 1383.215 kb on - strandat 1383.373 kb on + strandat 1383.373 kb on + strandat 1383.403 kb on + strandat 1383.403 kb on + strandat 1383.403 kb on + strandat 1383.404 kb on - strandat 1383.583 kb on + strand, within RR42_RS06300at 1383.584 kb on - strand, within RR42_RS06300at 1383.715 kb on + strand, within RR42_RS06300at 1383.715 kb on + strand, within RR42_RS06300at 1383.781 kb on + strand, within RR42_RS06300at 1383.968 kb on - strand, within RR42_RS06300at 1383.968 kb on - strand, within RR42_RS06300at 1383.968 kb on - strand, within RR42_RS06300at 1384.223 kb on - strand, within RR42_RS06300at 1384.546 kb on + strand, within RR42_RS06300at 1384.552 kb on + strand, within RR42_RS06300at 1384.552 kb on + strand, within RR42_RS06300at 1384.552 kb on + strand, within RR42_RS06300at 1384.715 kb on + strandat 1384.776 kb on + strandat 1384.824 kb on - strandat 1384.962 kb on - strand, within RR42_RS06305at 1385.001 kb on - strand, within RR42_RS06305at 1385.001 kb on - strand, within RR42_RS06305at 1385.001 kb on - strand, within RR42_RS06305at 1385.001 kb on - strand, within RR42_RS06305at 1385.081 kb on + strand, within RR42_RS06305at 1385.081 kb on + strand, within RR42_RS06305at 1385.082 kb on - strand, within RR42_RS06305at 1385.082 kb on - strand, within RR42_RS06305at 1385.082 kb on - strand, within RR42_RS06305at 1385.082 kb on - strand, within RR42_RS06305at 1385.082 kb on - strand, within RR42_RS06305at 1385.207 kb on + strand, within RR42_RS06305at 1385.207 kb on + strand, within RR42_RS06305at 1385.225 kb on + strand, within RR42_RS06305at 1385.226 kb on - strand, within RR42_RS06305at 1385.226 kb on - strand, within RR42_RS06305at 1385.516 kb on + strandat 1385.517 kb on - strandat 1385.517 kb on - strandat 1385.517 kb on - strandat 1385.686 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,382,264 - RR42_RS06295 0.58 -0.2
1,382,312 - RR42_RS06295 0.60 -0.1
1,382,312 - RR42_RS06295 0.60 +0.6
1,382,312 - RR42_RS06295 0.60 +0.5
1,382,312 - RR42_RS06295 0.60 -1.0
1,382,392 + RR42_RS06295 0.64 -0.1
1,382,392 + RR42_RS06295 0.64 +0.1
1,382,393 - RR42_RS06295 0.64 +0.4
1,382,397 - RR42_RS06295 0.64 -2.1
1,382,679 + RR42_RS06295 0.79 -0.8
1,382,854 + RR42_RS06295 0.88 -0.7
1,382,874 + RR42_RS06295 0.89 +0.9
1,382,874 + RR42_RS06295 0.89 -1.0
1,382,875 - RR42_RS06295 0.89 +0.2
1,382,918 + -1.4
1,382,919 - +0.1
1,382,957 - +0.3
1,383,214 + -0.8
1,383,214 + -0.0
1,383,214 + -0.1
1,383,214 + +0.4
1,383,215 - +0.7
1,383,215 - -1.7
1,383,215 - -0.4
1,383,373 + -2.2
1,383,373 + -0.7
1,383,403 + +0.1
1,383,403 + -1.4
1,383,403 + -0.4
1,383,404 - -0.3
1,383,583 + RR42_RS06300 0.22 +1.1
1,383,584 - RR42_RS06300 0.22 +0.1
1,383,715 + RR42_RS06300 0.31 -1.3
1,383,715 + RR42_RS06300 0.31 -3.0
1,383,781 + RR42_RS06300 0.35 -0.4
1,383,968 - RR42_RS06300 0.48 +0.9
1,383,968 - RR42_RS06300 0.48 +0.9
1,383,968 - RR42_RS06300 0.48 +0.8
1,384,223 - RR42_RS06300 0.65 -0.9
1,384,546 + RR42_RS06300 0.87 -2.1
1,384,552 + RR42_RS06300 0.87 -0.7
1,384,552 + RR42_RS06300 0.87 -2.3
1,384,552 + RR42_RS06300 0.87 -0.0
1,384,715 + +0.5
1,384,776 + +0.9
1,384,824 - -0.4
1,384,962 - RR42_RS06305 0.11 +0.4
1,385,001 - RR42_RS06305 0.17 -0.3
1,385,001 - RR42_RS06305 0.17 -0.7
1,385,001 - RR42_RS06305 0.17 -0.9
1,385,001 - RR42_RS06305 0.17 -0.5
1,385,081 + RR42_RS06305 0.29 -0.6
1,385,081 + RR42_RS06305 0.29 -0.2
1,385,082 - RR42_RS06305 0.29 -1.0
1,385,082 - RR42_RS06305 0.29 +0.9
1,385,082 - RR42_RS06305 0.29 +1.1
1,385,082 - RR42_RS06305 0.29 +0.1
1,385,082 - RR42_RS06305 0.29 -1.1
1,385,207 + RR42_RS06305 0.49 +1.4
1,385,207 + RR42_RS06305 0.49 -1.1
1,385,225 + RR42_RS06305 0.52 -0.3
1,385,226 - RR42_RS06305 0.52 +1.6
1,385,226 - RR42_RS06305 0.52 -0.0
1,385,516 + +0.0
1,385,517 - -1.1
1,385,517 - +1.0
1,385,517 - -0.8
1,385,686 + +2.8

Or see this region's nucleotide sequence