Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05975

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05965 and RR42_RS05970 are separated by 72 nucleotidesRR42_RS05970 and RR42_RS05975 overlap by 1 nucleotidesRR42_RS05975 and RR42_RS05980 are separated by 189 nucleotidesRR42_RS05980 and RR42_RS05985 are separated by 79 nucleotides RR42_RS05965: RR42_RS05965 - malonic semialdehyde reductase, at 1,312,279 to 1,312,866 _RS05965 RR42_RS05970: RR42_RS05970 - endonuclease III, at 1,312,939 to 1,313,583 _RS05970 RR42_RS05975: RR42_RS05975 - ferredoxin, at 1,313,583 to 1,314,404 _RS05975 RR42_RS05980: RR42_RS05980 - TetR family transcriptional regulator, at 1,314,594 to 1,315,229 _RS05980 RR42_RS05985: RR42_RS05985 - esterase, at 1,315,309 to 1,316,550 _RS05985 Position (kb) 1313 1314 1315Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1312.667 kb on - strand, within RR42_RS05965at 1312.889 kb on - strandat 1312.889 kb on - strandat 1312.889 kb on - strandat 1312.970 kb on + strandat 1313.030 kb on + strand, within RR42_RS05970at 1313.030 kb on + strand, within RR42_RS05970at 1313.031 kb on - strand, within RR42_RS05970at 1313.163 kb on + strand, within RR42_RS05970at 1313.163 kb on + strandat 1313.163 kb on + strand, within RR42_RS05970at 1313.164 kb on - strand, within RR42_RS05970at 1313.164 kb on - strand, within RR42_RS05970at 1313.164 kb on - strand, within RR42_RS05970at 1313.209 kb on + strand, within RR42_RS05970at 1313.314 kb on + strand, within RR42_RS05970at 1313.314 kb on + strand, within RR42_RS05970at 1313.314 kb on + strand, within RR42_RS05970at 1313.314 kb on + strand, within RR42_RS05970at 1313.314 kb on + strand, within RR42_RS05970at 1313.315 kb on - strand, within RR42_RS05970at 1313.315 kb on - strand, within RR42_RS05970at 1313.315 kb on - strand, within RR42_RS05970at 1313.340 kb on - strand, within RR42_RS05970at 1313.340 kb on - strand, within RR42_RS05970at 1313.360 kb on + strand, within RR42_RS05970at 1313.360 kb on + strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.361 kb on - strand, within RR42_RS05970at 1313.476 kb on + strand, within RR42_RS05970at 1313.476 kb on + strand, within RR42_RS05970at 1313.477 kb on - strand, within RR42_RS05970at 1313.477 kb on - strand, within RR42_RS05970at 1313.477 kb on - strand, within RR42_RS05970at 1313.477 kb on - strand, within RR42_RS05970at 1313.507 kb on + strand, within RR42_RS05970at 1313.507 kb on + strand, within RR42_RS05970at 1313.507 kb on + strand, within RR42_RS05970at 1313.508 kb on - strand, within RR42_RS05970at 1313.508 kb on - strand, within RR42_RS05970at 1313.585 kb on - strandat 1313.585 kb on - strandat 1313.648 kb on + strandat 1313.927 kb on - strand, within RR42_RS05975at 1314.071 kb on + strand, within RR42_RS05975at 1314.071 kb on + strand, within RR42_RS05975at 1314.310 kb on + strand, within RR42_RS05975at 1314.744 kb on + strand, within RR42_RS05980at 1314.744 kb on + strand, within RR42_RS05980at 1314.744 kb on + strand, within RR42_RS05980at 1314.744 kb on + strand, within RR42_RS05980at 1314.745 kb on - strand, within RR42_RS05980at 1314.745 kb on - strand, within RR42_RS05980at 1314.747 kb on + strand, within RR42_RS05980at 1314.747 kb on + strand, within RR42_RS05980at 1314.747 kb on + strand, within RR42_RS05980at 1314.748 kb on - strand, within RR42_RS05980at 1314.751 kb on - strand, within RR42_RS05980at 1314.753 kb on + strand, within RR42_RS05980at 1314.753 kb on + strand, within RR42_RS05980at 1315.075 kb on + strand, within RR42_RS05980at 1315.077 kb on + strand, within RR42_RS05980at 1315.078 kb on - strand, within RR42_RS05980at 1315.078 kb on - strand, within RR42_RS05980at 1315.078 kb on - strand, within RR42_RS05980at 1315.101 kb on + strand, within RR42_RS05980at 1315.102 kb on - strand, within RR42_RS05980at 1315.104 kb on + strand, within RR42_RS05980at 1315.104 kb on + strand, within RR42_RS05980at 1315.104 kb on + strand, within RR42_RS05980at 1315.105 kb on - strand, within RR42_RS05980at 1315.297 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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1,312,667 - RR42_RS05965 0.66 +0.8
1,312,889 - +0.1
1,312,889 - +0.7
1,312,889 - +1.8
1,312,970 + -1.1
1,313,030 + RR42_RS05970 0.14 -1.6
1,313,030 + RR42_RS05970 0.14 -0.0
1,313,031 - RR42_RS05970 0.14 -1.0
1,313,163 + RR42_RS05970 0.35 +0.4
1,313,163 + -0.6
1,313,163 + RR42_RS05970 0.35 +0.8
1,313,164 - RR42_RS05970 0.35 +0.9
1,313,164 - RR42_RS05970 0.35 +0.3
1,313,164 - RR42_RS05970 0.35 +0.2
1,313,209 + RR42_RS05970 0.42 +0.4
1,313,314 + RR42_RS05970 0.58 +0.5
1,313,314 + RR42_RS05970 0.58 +1.4
1,313,314 + RR42_RS05970 0.58 +1.7
1,313,314 + RR42_RS05970 0.58 +0.5
1,313,314 + RR42_RS05970 0.58 -0.3
1,313,315 - RR42_RS05970 0.58 +0.2
1,313,315 - RR42_RS05970 0.58 -0.6
1,313,315 - RR42_RS05970 0.58 -0.8
1,313,340 - RR42_RS05970 0.62 -0.1
1,313,340 - RR42_RS05970 0.62 +0.1
1,313,360 + RR42_RS05970 0.65 -0.3
1,313,360 + RR42_RS05970 0.65 +0.4
1,313,361 - RR42_RS05970 0.65 -0.9
1,313,361 - RR42_RS05970 0.65 +0.8
1,313,361 - RR42_RS05970 0.65 -1.9
1,313,361 - RR42_RS05970 0.65 +0.2
1,313,361 - RR42_RS05970 0.65 -2.5
1,313,361 - RR42_RS05970 0.65 +0.8
1,313,361 - RR42_RS05970 0.65 -0.2
1,313,476 + RR42_RS05970 0.83 -0.2
1,313,476 + RR42_RS05970 0.83 +0.1
1,313,477 - RR42_RS05970 0.83 +0.1
1,313,477 - RR42_RS05970 0.83 -2.8
1,313,477 - RR42_RS05970 0.83 -0.2
1,313,477 - RR42_RS05970 0.83 -0.2
1,313,507 + RR42_RS05970 0.88 +0.6
1,313,507 + RR42_RS05970 0.88 -1.5
1,313,507 + RR42_RS05970 0.88 -0.8
1,313,508 - RR42_RS05970 0.88 -3.2
1,313,508 - RR42_RS05970 0.88 -0.1
1,313,585 - -1.6
1,313,585 - +0.6
1,313,648 + -0.7
1,313,927 - RR42_RS05975 0.42 -2.2
1,314,071 + RR42_RS05975 0.59 -0.3
1,314,071 + RR42_RS05975 0.59 +1.5
1,314,310 + RR42_RS05975 0.88 -1.1
1,314,744 + RR42_RS05980 0.24 +1.8
1,314,744 + RR42_RS05980 0.24 -1.6
1,314,744 + RR42_RS05980 0.24 -1.6
1,314,744 + RR42_RS05980 0.24 -0.5
1,314,745 - RR42_RS05980 0.24 -3.8
1,314,745 - RR42_RS05980 0.24 +0.7
1,314,747 + RR42_RS05980 0.24 +1.1
1,314,747 + RR42_RS05980 0.24 -0.9
1,314,747 + RR42_RS05980 0.24 +0.2
1,314,748 - RR42_RS05980 0.24 -1.4
1,314,751 - RR42_RS05980 0.25 -1.7
1,314,753 + RR42_RS05980 0.25 -1.6
1,314,753 + RR42_RS05980 0.25 -0.3
1,315,075 + RR42_RS05980 0.76 -0.6
1,315,077 + RR42_RS05980 0.76 +0.3
1,315,078 - RR42_RS05980 0.76 +0.3
1,315,078 - RR42_RS05980 0.76 +0.3
1,315,078 - RR42_RS05980 0.76 -3.0
1,315,101 + RR42_RS05980 0.80 -3.1
1,315,102 - RR42_RS05980 0.80 -0.1
1,315,104 + RR42_RS05980 0.80 +0.7
1,315,104 + RR42_RS05980 0.80 +0.2
1,315,104 + RR42_RS05980 0.80 -0.1
1,315,105 - RR42_RS05980 0.80 +1.3
1,315,297 - +2.3

Or see this region's nucleotide sequence