Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05615 and RR42_RS05620 are separated by 9 nucleotides RR42_RS05620 and RR42_RS05625 are separated by 181 nucleotides RR42_RS05625 and RR42_RS05630 are separated by 345 nucleotides
RR42_RS05615: RR42_RS05615 - MFS transporter, at 1,235,458 to 1,236,759
_RS05615
RR42_RS05620: RR42_RS05620 - esterase, at 1,236,769 to 1,237,527
_RS05620
RR42_RS05625: RR42_RS05625 - 4-hydroxyacetophenone monooxygenase, at 1,237,709 to 1,239,196
_RS05625
RR42_RS05630: RR42_RS05630 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,239,542 to 1,240,807
_RS05630
Position (kb)
1237
1238
1239
1240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1236.740 kb on + strand at 1236.740 kb on + strand at 1236.740 kb on + strand at 1236.741 kb on - strand at 1236.753 kb on + strand at 1236.902 kb on + strand, within RR42_RS05620 at 1236.902 kb on + strand, within RR42_RS05620 at 1237.323 kb on - strand, within RR42_RS05620 at 1237.711 kb on - strand at 1237.711 kb on - strand at 1237.776 kb on + strand at 1237.777 kb on - strand at 1237.831 kb on - strand at 1238.023 kb on + strand, within RR42_RS05625 at 1238.024 kb on - strand, within RR42_RS05625 at 1238.079 kb on + strand, within RR42_RS05625 at 1238.079 kb on + strand, within RR42_RS05625 at 1238.079 kb on + strand, within RR42_RS05625 at 1238.144 kb on - strand, within RR42_RS05625 at 1238.391 kb on + strand, within RR42_RS05625 at 1238.539 kb on + strand, within RR42_RS05625 at 1238.539 kb on + strand, within RR42_RS05625 at 1238.539 kb on + strand, within RR42_RS05625 at 1238.539 kb on + strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.540 kb on - strand, within RR42_RS05625 at 1238.541 kb on + strand, within RR42_RS05625 at 1238.588 kb on + strand, within RR42_RS05625 at 1238.856 kb on + strand, within RR42_RS05625 at 1238.890 kb on + strand, within RR42_RS05625 at 1238.932 kb on - strand, within RR42_RS05625 at 1238.959 kb on - strand, within RR42_RS05625 at 1238.998 kb on + strand, within RR42_RS05625 at 1238.999 kb on - strand, within RR42_RS05625 at 1238.999 kb on - strand, within RR42_RS05625 at 1239.000 kb on + strand, within RR42_RS05625 at 1239.219 kb on + strand at 1239.275 kb on + strand at 1239.387 kb on + strand at 1239.450 kb on + strand at 1239.451 kb on - strand at 1239.451 kb on - strand at 1239.452 kb on + strand at 1239.452 kb on + strand at 1239.452 kb on + strand at 1239.477 kb on + strand at 1239.477 kb on + strand at 1239.478 kb on - strand at 1239.478 kb on - strand at 1239.845 kb on - strand, within RR42_RS05630 at 1239.933 kb on - strand, within RR42_RS05630 at 1239.945 kb on - strand, within RR42_RS05630 at 1239.977 kb on + strand, within RR42_RS05630 at 1240.079 kb on + strand, within RR42_RS05630 at 1240.079 kb on + strand, within RR42_RS05630 at 1240.080 kb on - strand, within RR42_RS05630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,236,740 + +0.1 1,236,740 + +0.5 1,236,740 + +0.5 1,236,741 - +1.1 1,236,753 + +0.2 1,236,902 + RR42_RS05620 0.18 -0.7 1,236,902 + RR42_RS05620 0.18 -0.9 1,237,323 - RR42_RS05620 0.73 +1.0 1,237,711 - -2.2 1,237,711 - +0.7 1,237,776 + +0.2 1,237,777 - -0.1 1,237,831 - +0.3 1,238,023 + RR42_RS05625 0.21 -1.4 1,238,024 - RR42_RS05625 0.21 -1.8 1,238,079 + RR42_RS05625 0.25 +0.1 1,238,079 + RR42_RS05625 0.25 +0.1 1,238,079 + RR42_RS05625 0.25 +0.3 1,238,144 - RR42_RS05625 0.29 +0.5 1,238,391 + RR42_RS05625 0.46 -0.9 1,238,539 + RR42_RS05625 0.56 +0.1 1,238,539 + RR42_RS05625 0.56 +0.5 1,238,539 + RR42_RS05625 0.56 +0.3 1,238,539 + RR42_RS05625 0.56 -0.2 1,238,540 - RR42_RS05625 0.56 +0.1 1,238,540 - RR42_RS05625 0.56 +1.7 1,238,540 - RR42_RS05625 0.56 -1.5 1,238,540 - RR42_RS05625 0.56 +0.7 1,238,540 - RR42_RS05625 0.56 -0.7 1,238,540 - RR42_RS05625 0.56 +0.5 1,238,541 + RR42_RS05625 0.56 -2.5 1,238,588 + RR42_RS05625 0.59 -2.2 1,238,856 + RR42_RS05625 0.77 +0.5 1,238,890 + RR42_RS05625 0.79 -0.1 1,238,932 - RR42_RS05625 0.82 -0.6 1,238,959 - RR42_RS05625 0.84 -0.3 1,238,998 + RR42_RS05625 0.87 -0.0 1,238,999 - RR42_RS05625 0.87 -0.5 1,238,999 - RR42_RS05625 0.87 -0.3 1,239,000 + RR42_RS05625 0.87 -1.0 1,239,219 + -0.2 1,239,275 + -0.9 1,239,387 + +1.0 1,239,450 + +0.3 1,239,451 - +0.3 1,239,451 - +1.3 1,239,452 + +0.2 1,239,452 + -0.5 1,239,452 + -1.6 1,239,477 + -1.0 1,239,477 + -0.2 1,239,478 - -0.2 1,239,478 - +0.7 1,239,845 - RR42_RS05630 0.24 -2.0 1,239,933 - RR42_RS05630 0.31 -3.1 1,239,945 - RR42_RS05630 0.32 +0.9 1,239,977 + RR42_RS05630 0.34 -0.3 1,240,079 + RR42_RS05630 0.42 -1.2 1,240,079 + RR42_RS05630 0.42 -1.6 1,240,080 - RR42_RS05630 0.42 -0.0
Or see this region's nucleotide sequence