Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05415 and RR42_RS05420 are separated by 193 nucleotides RR42_RS05420 and RR42_RS05425 are separated by 2 nucleotides RR42_RS05425 and RR42_RS05430 overlap by 4 nucleotides RR42_RS05430 and RR42_RS05435 are separated by 368 nucleotides
RR42_RS05415: RR42_RS05415 - membrane protein, at 1,196,313 to 1,196,879
_RS05415
RR42_RS05420: RR42_RS05420 - membrane protein, at 1,197,073 to 1,197,852
_RS05420
RR42_RS05425: RR42_RS05425 - membrane protein, at 1,197,855 to 1,198,289
_RS05425
RR42_RS05430: RR42_RS05430 - DNA-directed RNA polymerase sigma-70 factor, at 1,198,286 to 1,198,852
_RS05430
RR42_RS05435: RR42_RS05435 - acetolactate synthase 3 catalytic subunit, at 1,199,221 to 1,200,981
_RS05435
Position (kb) 1197
1198
1199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1196.995 kb on + strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1197.416 kb on + strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.471 kb on - strand, within RR42_RS05420 at 1197.857 kb on - strand at 1197.857 kb on - strand at 1197.876 kb on + strand at 1197.876 kb on + strand at 1197.877 kb on - strand at 1197.899 kb on - strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1198.406 kb on - strand, within RR42_RS05430 at 1198.567 kb on + strand, within RR42_RS05430 at 1198.619 kb on - strand, within RR42_RS05430 at 1198.626 kb on - strand, within RR42_RS05430 at 1198.667 kb on + strand, within RR42_RS05430 at 1198.668 kb on - strand, within RR42_RS05430 at 1198.832 kb on + strand at 1198.832 kb on + strand at 1198.901 kb on - strand at 1198.934 kb on + strand at 1198.934 kb on + strand at 1198.935 kb on - strand at 1198.941 kb on + strand at 1198.941 kb on + strand at 1198.942 kb on - strand at 1199.130 kb on + strand at 1199.130 kb on + strand at 1199.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,196,995 + -1.0 1,196,996 - -0.4 1,196,996 - -0.0 1,196,996 - -1.3 1,197,416 + RR42_RS05420 0.44 -5.5 1,197,417 - RR42_RS05420 0.44 -5.4 1,197,417 - RR42_RS05420 0.44 -4.4 1,197,471 - RR42_RS05420 0.51 -2.4 1,197,857 - -4.0 1,197,857 - -1.0 1,197,876 + -2.2 1,197,876 + +0.0 1,197,877 - -2.4 1,197,899 - RR42_RS05425 0.10 +0.0 1,197,982 + RR42_RS05425 0.29 -1.0 1,197,982 + RR42_RS05425 0.29 -3.2 1,197,983 - RR42_RS05425 0.29 -1.2 1,197,983 - RR42_RS05425 0.29 -1.0 1,197,983 - RR42_RS05425 0.29 -0.7 1,197,983 - RR42_RS05425 0.29 -1.7 1,197,983 - RR42_RS05425 0.29 -0.7 1,197,983 - RR42_RS05425 0.29 -3.3 1,197,983 - RR42_RS05425 0.29 -1.9 1,197,983 - RR42_RS05425 0.29 -2.2 1,198,406 - RR42_RS05430 0.21 +1.1 1,198,567 + RR42_RS05430 0.50 +1.7 1,198,619 - RR42_RS05430 0.59 +2.0 1,198,626 - RR42_RS05430 0.60 -0.4 1,198,667 + RR42_RS05430 0.67 +1.7 1,198,668 - RR42_RS05430 0.67 +0.0 1,198,832 + +2.0 1,198,832 + +0.8 1,198,901 - +1.1 1,198,934 + +0.5 1,198,934 + +0.9 1,198,935 - +1.4 1,198,941 + +0.6 1,198,941 + +1.9 1,198,942 - -0.3 1,199,130 + +2.2 1,199,130 + -0.1 1,199,130 + -0.4
Or see this region's nucleotide sequence