Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05245 and RR42_RS05250 are separated by 276 nucleotides RR42_RS05250 and RR42_RS05255 are separated by 8 nucleotides RR42_RS05255 and RR42_RS05260 are separated by 48 nucleotides
RR42_RS05245: RR42_RS05245 - porin, at 1,158,016 to 1,159,101
_RS05245
RR42_RS05250: RR42_RS05250 - OHCU decarboxylase, at 1,159,378 to 1,159,896
_RS05250
RR42_RS05255: RR42_RS05255 - allantoinase, at 1,159,905 to 1,160,855
_RS05255
RR42_RS05260: RR42_RS05260 - purine permease, at 1,160,904 to 1,162,310
_RS05260
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1158.914 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1159.008 kb on - strand at 1159.096 kb on - strand at 1159.141 kb on + strand at 1159.163 kb on - strand at 1159.165 kb on + strand at 1159.165 kb on + strand at 1159.299 kb on + strand at 1159.321 kb on + strand at 1159.390 kb on + strand at 1159.390 kb on + strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.554 kb on - strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.883 kb on + strand at 1159.883 kb on + strand at 1159.884 kb on - strand at 1160.116 kb on + strand, within RR42_RS05255 at 1160.117 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.216 kb on - strand, within RR42_RS05255 at 1160.291 kb on - strand, within RR42_RS05255 at 1160.294 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.459 kb on - strand, within RR42_RS05255 at 1160.459 kb on - strand, within RR42_RS05255 at 1160.632 kb on + strand, within RR42_RS05255 at 1160.633 kb on - strand, within RR42_RS05255 at 1160.750 kb on - strand, within RR42_RS05255 at 1160.750 kb on - strand, within RR42_RS05255 at 1160.839 kb on + strand at 1160.905 kb on + strand at 1160.906 kb on - strand at 1160.906 kb on - strand at 1161.053 kb on - strand, within RR42_RS05260 at 1161.053 kb on - strand, within RR42_RS05260 at 1161.358 kb on + strand, within RR42_RS05260 at 1161.359 kb on - strand, within RR42_RS05260 at 1161.694 kb on + strand, within RR42_RS05260 at 1161.695 kb on - strand, within RR42_RS05260 at 1161.760 kb on + strand, within RR42_RS05260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,158,914 + RR42_RS05245 0.83 -1.3 1,158,947 + RR42_RS05245 0.86 +0.5 1,158,947 + RR42_RS05245 0.86 +0.1 1,158,947 + RR42_RS05245 0.86 +0.4 1,159,008 - -1.8 1,159,096 - +0.1 1,159,141 + +0.4 1,159,163 - -2.1 1,159,165 + -1.0 1,159,165 + -1.0 1,159,299 + +1.0 1,159,321 + +0.2 1,159,390 + +0.8 1,159,390 + +0.2 1,159,391 - +1.9 1,159,391 - +0.8 1,159,391 - +0.9 1,159,391 - +0.2 1,159,391 - -0.3 1,159,554 - RR42_RS05250 0.34 +0.7 1,159,820 + RR42_RS05250 0.85 -0.2 1,159,820 + RR42_RS05250 0.85 +1.8 1,159,820 + RR42_RS05250 0.85 +0.8 1,159,821 - RR42_RS05250 0.85 -0.6 1,159,821 - RR42_RS05250 0.85 -0.9 1,159,821 - RR42_RS05250 0.85 -1.1 1,159,821 - RR42_RS05250 0.85 +0.5 1,159,821 - RR42_RS05250 0.85 +0.1 1,159,821 - RR42_RS05250 0.85 -0.4 1,159,821 - RR42_RS05250 0.85 +0.0 1,159,883 + +2.6 1,159,883 + +0.2 1,159,884 - -0.2 1,160,116 + RR42_RS05255 0.22 -0.6 1,160,117 - RR42_RS05255 0.22 -0.7 1,160,214 - RR42_RS05255 0.32 +0.0 1,160,214 - RR42_RS05255 0.32 +0.4 1,160,214 - RR42_RS05255 0.32 -1.8 1,160,216 - RR42_RS05255 0.33 -2.1 1,160,291 - RR42_RS05255 0.41 -1.4 1,160,294 - RR42_RS05255 0.41 -0.4 1,160,306 - RR42_RS05255 0.42 +2.0 1,160,306 - RR42_RS05255 0.42 -0.2 1,160,306 - RR42_RS05255 0.42 -1.0 1,160,459 - RR42_RS05255 0.58 +0.0 1,160,459 - RR42_RS05255 0.58 -2.4 1,160,632 + RR42_RS05255 0.76 -0.2 1,160,633 - RR42_RS05255 0.77 +0.0 1,160,750 - RR42_RS05255 0.89 -1.3 1,160,750 - RR42_RS05255 0.89 -0.2 1,160,839 + -1.3 1,160,905 + -0.2 1,160,906 - +0.1 1,160,906 - -1.3 1,161,053 - RR42_RS05260 0.11 +0.4 1,161,053 - RR42_RS05260 0.11 -0.2 1,161,358 + RR42_RS05260 0.32 -2.0 1,161,359 - RR42_RS05260 0.32 +1.0 1,161,694 + RR42_RS05260 0.56 +1.5 1,161,695 - RR42_RS05260 0.56 -1.5 1,161,760 + RR42_RS05260 0.61 +2.4
Or see this region's nucleotide sequence