Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05000 and RR42_RS05005 are separated by 21 nucleotides RR42_RS05005 and RR42_RS05010 are separated by 145 nucleotides RR42_RS05010 and RR42_RS05015 are separated by 70 nucleotides
RR42_RS05000: RR42_RS05000 - hypothetical protein, at 1,103,869 to 1,105,491
_RS05000
RR42_RS05005: RR42_RS05005 - NAD(FAD)-utilizing dehydrogenase, at 1,105,513 to 1,106,781
_RS05005
RR42_RS05010: RR42_RS05010 - hypothetical protein, at 1,106,927 to 1,107,319
_RS05010
RR42_RS05015: RR42_RS05015 - peptidase, at 1,107,390 to 1,107,956
_RS05015
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1104.829 kb on + strand, within RR42_RS05000 at 1104.830 kb on - strand, within RR42_RS05000 at 1104.868 kb on + strand, within RR42_RS05000 at 1104.868 kb on + strand, within RR42_RS05000 at 1104.869 kb on - strand, within RR42_RS05000 at 1104.952 kb on + strand, within RR42_RS05000 at 1104.953 kb on - strand, within RR42_RS05000 at 1105.090 kb on + strand, within RR42_RS05000 at 1105.090 kb on + strand, within RR42_RS05000 at 1105.091 kb on - strand, within RR42_RS05000 at 1105.091 kb on - strand, within RR42_RS05000 at 1105.168 kb on + strand, within RR42_RS05000 at 1105.168 kb on + strand, within RR42_RS05000 at 1105.169 kb on - strand, within RR42_RS05000 at 1105.169 kb on - strand, within RR42_RS05000 at 1105.216 kb on + strand, within RR42_RS05000 at 1105.217 kb on - strand, within RR42_RS05000 at 1105.351 kb on + strand at 1105.352 kb on - strand at 1105.352 kb on - strand at 1105.352 kb on - strand at 1105.352 kb on - strand at 1105.352 kb on - strand at 1105.352 kb on - strand at 1105.417 kb on + strand at 1105.417 kb on + strand at 1105.417 kb on + strand at 1105.418 kb on - strand at 1105.510 kb on + strand at 1105.510 kb on + strand at 1105.510 kb on + strand at 1106.158 kb on + strand, within RR42_RS05005 at 1106.159 kb on - strand, within RR42_RS05005 at 1106.159 kb on - strand, within RR42_RS05005 at 1106.373 kb on + strand, within RR42_RS05005 at 1106.374 kb on - strand, within RR42_RS05005 at 1106.374 kb on - strand, within RR42_RS05005 at 1106.568 kb on - strand, within RR42_RS05005 at 1106.667 kb on - strand at 1106.850 kb on - strand at 1106.850 kb on - strand at 1106.850 kb on - strand at 1106.850 kb on - strand at 1106.851 kb on + strand at 1106.851 kb on + strand at 1106.851 kb on + strand at 1106.852 kb on - strand at 1106.852 kb on - strand at 1106.852 kb on - strand at 1106.852 kb on - strand at 1106.852 kb on - strand at 1106.853 kb on + strand at 1106.853 kb on + strand at 1106.853 kb on + strand at 1106.854 kb on - strand at 1106.854 kb on - strand at 1106.871 kb on + strand at 1106.872 kb on - strand at 1106.872 kb on - strand at 1106.872 kb on - strand at 1106.872 kb on - strand at 1106.874 kb on + strand at 1107.000 kb on + strand, within RR42_RS05010 at 1107.000 kb on + strand, within RR42_RS05010 at 1107.000 kb on + strand, within RR42_RS05010 at 1107.000 kb on + strand, within RR42_RS05010 at 1107.001 kb on - strand, within RR42_RS05010 at 1107.001 kb on - strand, within RR42_RS05010 at 1107.104 kb on + strand, within RR42_RS05010 at 1107.227 kb on + strand, within RR42_RS05010 at 1107.227 kb on + strand, within RR42_RS05010 at 1107.227 kb on + strand, within RR42_RS05010 at 1107.228 kb on - strand, within RR42_RS05010 at 1107.228 kb on - strand, within RR42_RS05010 at 1107.302 kb on + strand at 1107.348 kb on + strand at 1107.349 kb on - strand at 1107.467 kb on + strand, within RR42_RS05015 at 1107.468 kb on - strand, within RR42_RS05015 at 1107.657 kb on + strand, within RR42_RS05015 at 1107.672 kb on + strand, within RR42_RS05015 at 1107.672 kb on + strand, within RR42_RS05015 at 1107.745 kb on - strand, within RR42_RS05015 at 1107.745 kb on - strand, within RR42_RS05015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,104,829 + RR42_RS05000 0.59 -3.8 1,104,830 - RR42_RS05000 0.59 -1.8 1,104,868 + RR42_RS05000 0.62 +0.9 1,104,868 + RR42_RS05000 0.62 -0.5 1,104,869 - RR42_RS05000 0.62 +0.7 1,104,952 + RR42_RS05000 0.67 +0.1 1,104,953 - RR42_RS05000 0.67 +0.7 1,105,090 + RR42_RS05000 0.75 -2.0 1,105,090 + RR42_RS05000 0.75 +0.7 1,105,091 - RR42_RS05000 0.75 +0.5 1,105,091 - RR42_RS05000 0.75 -2.8 1,105,168 + RR42_RS05000 0.80 -1.9 1,105,168 + RR42_RS05000 0.80 -2.1 1,105,169 - RR42_RS05000 0.80 +0.0 1,105,169 - RR42_RS05000 0.80 -1.3 1,105,216 + RR42_RS05000 0.83 -0.1 1,105,217 - RR42_RS05000 0.83 -0.1 1,105,351 + -0.0 1,105,352 - -0.5 1,105,352 - -1.3 1,105,352 - +0.6 1,105,352 - +0.4 1,105,352 - -2.8 1,105,352 - +0.1 1,105,417 + +1.1 1,105,417 + -0.3 1,105,417 + -0.4 1,105,418 - -1.6 1,105,510 + -0.2 1,105,510 + -0.3 1,105,510 + +0.0 1,106,158 + RR42_RS05005 0.51 -0.6 1,106,159 - RR42_RS05005 0.51 -0.3 1,106,159 - RR42_RS05005 0.51 +0.1 1,106,373 + RR42_RS05005 0.68 -1.6 1,106,374 - RR42_RS05005 0.68 -0.3 1,106,374 - RR42_RS05005 0.68 -0.9 1,106,568 - RR42_RS05005 0.83 -1.8 1,106,667 - -1.9 1,106,850 - +0.8 1,106,850 - +2.1 1,106,850 - +0.4 1,106,850 - -0.7 1,106,851 + -0.3 1,106,851 + -0.1 1,106,851 + +0.2 1,106,852 - -1.0 1,106,852 - +0.4 1,106,852 - +0.5 1,106,852 - -1.9 1,106,852 - -2.3 1,106,853 + -1.1 1,106,853 + +2.1 1,106,853 + -2.3 1,106,854 - -0.2 1,106,854 - -0.6 1,106,871 + -0.5 1,106,872 - +1.5 1,106,872 - -1.0 1,106,872 - -1.0 1,106,872 - +0.9 1,106,874 + -2.8 1,107,000 + RR42_RS05010 0.19 -0.1 1,107,000 + RR42_RS05010 0.19 -0.3 1,107,000 + RR42_RS05010 0.19 -1.1 1,107,000 + RR42_RS05010 0.19 +0.5 1,107,001 - RR42_RS05010 0.19 -0.4 1,107,001 - RR42_RS05010 0.19 +0.6 1,107,104 + RR42_RS05010 0.45 -0.6 1,107,227 + RR42_RS05010 0.76 +0.8 1,107,227 + RR42_RS05010 0.76 -0.2 1,107,227 + RR42_RS05010 0.76 -0.3 1,107,228 - RR42_RS05010 0.77 -0.4 1,107,228 - RR42_RS05010 0.77 -1.1 1,107,302 + -0.9 1,107,348 + +0.3 1,107,349 - +1.1 1,107,467 + RR42_RS05015 0.14 -2.1 1,107,468 - RR42_RS05015 0.14 -0.7 1,107,657 + RR42_RS05015 0.47 -0.8 1,107,672 + RR42_RS05015 0.50 -1.0 1,107,672 + RR42_RS05015 0.50 -4.1 1,107,745 - RR42_RS05015 0.63 -0.8 1,107,745 - RR42_RS05015 0.63 +0.4
Or see this region's nucleotide sequence