Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04760 and RR42_RS04765 are separated by 121 nucleotides RR42_RS04765 and RR42_RS04770 are separated by 72 nucleotides RR42_RS04770 and RR42_RS04775 are separated by 79 nucleotides
RR42_RS04760: RR42_RS04760 - multidrug DMT transporter permease, at 1,060,510 to 1,061,400
_RS04760
RR42_RS04765: RR42_RS04765 - 3-oxoacyl-ACP reductase, at 1,061,522 to 1,062,310
_RS04765
RR42_RS04770: RR42_RS04770 - methylglyoxal synthase, at 1,062,383 to 1,062,769
_RS04770
RR42_RS04775: RR42_RS04775 - quinone oxidoreductase, at 1,062,849 to 1,063,823
_RS04775
Position (kb)
1062
1063 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.383 kb on + strand at 1061.384 kb on - strand at 1061.384 kb on - strand at 1061.384 kb on - strand at 1061.384 kb on - strand at 1061.529 kb on + strand at 1061.529 kb on + strand at 1061.530 kb on + strand at 1062.091 kb on + strand, within RR42_RS04765 at 1062.104 kb on + strand, within RR42_RS04765 at 1062.105 kb on - strand, within RR42_RS04765 at 1062.105 kb on - strand, within RR42_RS04765 at 1062.254 kb on + strand at 1062.254 kb on + strand at 1062.255 kb on - strand at 1062.255 kb on - strand at 1062.255 kb on - strand at 1062.294 kb on - strand at 1062.308 kb on + strand at 1062.308 kb on + strand at 1062.308 kb on + strand at 1062.309 kb on - strand at 1062.309 kb on - strand at 1062.370 kb on + strand at 1062.371 kb on - strand at 1062.371 kb on - strand at 1062.398 kb on - strand at 1062.661 kb on + strand, within RR42_RS04770 at 1062.759 kb on - strand at 1062.805 kb on + strand at 1062.806 kb on - strand at 1062.806 kb on - strand at 1062.806 kb on - strand at 1062.811 kb on + strand at 1062.811 kb on + strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.812 kb on - strand at 1062.815 kb on + strand at 1062.815 kb on + strand at 1062.815 kb on + strand at 1062.816 kb on - strand at 1062.816 kb on - strand at 1062.816 kb on - strand at 1062.816 kb on - strand at 1062.972 kb on + strand, within RR42_RS04775 at 1062.973 kb on - strand, within RR42_RS04775 at 1062.984 kb on + strand, within RR42_RS04775 at 1062.984 kb on + strand, within RR42_RS04775 at 1063.002 kb on + strand, within RR42_RS04775 at 1063.003 kb on - strand, within RR42_RS04775 at 1063.003 kb on - strand, within RR42_RS04775 at 1063.105 kb on - strand, within RR42_RS04775 at 1063.129 kb on - strand, within RR42_RS04775 at 1063.251 kb on + strand, within RR42_RS04775 at 1063.251 kb on + strand, within RR42_RS04775 at 1063.251 kb on + strand, within RR42_RS04775 at 1063.251 kb on + strand, within RR42_RS04775 at 1063.485 kb on + strand, within RR42_RS04775 at 1063.486 kb on - strand, within RR42_RS04775 at 1063.486 kb on - strand, within RR42_RS04775 at 1063.506 kb on - strand, within RR42_RS04775 at 1063.650 kb on + strand, within RR42_RS04775 at 1063.650 kb on + strand, within RR42_RS04775 at 1063.650 kb on + strand, within RR42_RS04775 at 1063.650 kb on + strand, within RR42_RS04775 at 1063.651 kb on - strand, within RR42_RS04775 at 1063.651 kb on - strand, within RR42_RS04775 at 1063.651 kb on - strand, within RR42_RS04775 at 1063.651 kb on - strand, within RR42_RS04775 at 1063.651 kb on - strand, within RR42_RS04775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,061,383 + +0.2 1,061,383 + +1.2 1,061,383 + -0.1 1,061,383 + +0.6 1,061,383 + -0.1 1,061,383 + -0.0 1,061,383 + +0.5 1,061,383 + +0.7 1,061,383 + -0.0 1,061,384 - +0.6 1,061,384 - -1.0 1,061,384 - +1.7 1,061,384 - -0.8 1,061,529 + +0.1 1,061,529 + +0.4 1,061,530 + +0.0 1,062,091 + RR42_RS04765 0.72 +1.8 1,062,104 + RR42_RS04765 0.74 +1.0 1,062,105 - RR42_RS04765 0.74 +0.2 1,062,105 - RR42_RS04765 0.74 +0.4 1,062,254 + +1.8 1,062,254 + +0.1 1,062,255 - -0.1 1,062,255 - -3.1 1,062,255 - +0.3 1,062,294 - +2.6 1,062,308 + -0.5 1,062,308 + +0.7 1,062,308 + -0.1 1,062,309 - -0.3 1,062,309 - -1.3 1,062,370 + +1.0 1,062,371 - -2.4 1,062,371 - +0.3 1,062,398 - +0.6 1,062,661 + RR42_RS04770 0.72 -1.4 1,062,759 - +1.4 1,062,805 + -0.6 1,062,806 - +0.3 1,062,806 - -2.0 1,062,806 - -1.8 1,062,811 + -2.5 1,062,811 + +0.0 1,062,812 - +0.1 1,062,812 - +0.1 1,062,812 - +0.6 1,062,812 - +2.0 1,062,812 - +0.3 1,062,812 - -0.2 1,062,812 - -0.1 1,062,812 - -2.8 1,062,812 - -0.6 1,062,815 + -2.4 1,062,815 + -0.4 1,062,815 + -1.8 1,062,816 - -2.1 1,062,816 - -0.0 1,062,816 - -1.6 1,062,816 - +0.6 1,062,972 + RR42_RS04775 0.13 -0.1 1,062,973 - RR42_RS04775 0.13 -0.7 1,062,984 + RR42_RS04775 0.14 -0.3 1,062,984 + RR42_RS04775 0.14 -1.0 1,063,002 + RR42_RS04775 0.16 +0.0 1,063,003 - RR42_RS04775 0.16 -3.5 1,063,003 - RR42_RS04775 0.16 +0.0 1,063,105 - RR42_RS04775 0.26 +1.5 1,063,129 - RR42_RS04775 0.29 +3.3 1,063,251 + RR42_RS04775 0.41 -0.3 1,063,251 + RR42_RS04775 0.41 +0.4 1,063,251 + RR42_RS04775 0.41 +2.0 1,063,251 + RR42_RS04775 0.41 +2.3 1,063,485 + RR42_RS04775 0.65 +1.6 1,063,486 - RR42_RS04775 0.65 -0.2 1,063,486 - RR42_RS04775 0.65 -1.8 1,063,506 - RR42_RS04775 0.67 -1.1 1,063,650 + RR42_RS04775 0.82 +0.8 1,063,650 + RR42_RS04775 0.82 -0.6 1,063,650 + RR42_RS04775 0.82 -1.6 1,063,650 + RR42_RS04775 0.82 -1.3 1,063,651 - RR42_RS04775 0.82 +0.0 1,063,651 - RR42_RS04775 0.82 -0.3 1,063,651 - RR42_RS04775 0.82 +0.0 1,063,651 - RR42_RS04775 0.82 -0.6 1,063,651 - RR42_RS04775 0.82 +0.8
Or see this region's nucleotide sequence