Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04735 and RR42_RS04740 are separated by 258 nucleotides RR42_RS04740 and RR42_RS04745 are separated by 438 nucleotides
RR42_RS04735: RR42_RS04735 - hypothetical protein, at 1,054,000 to 1,054,725
_RS04735
RR42_RS04740: RR42_RS04740 - ATP-binding protein, at 1,054,984 to 1,056,534
_RS04740
RR42_RS04745: RR42_RS04745 - uracil phosphoribosyltransferase, at 1,056,973 to 1,057,623
_RS04745
Position (kb)
1054
1055
1056
1057 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1054.482 kb on - strand, within RR42_RS04735 at 1054.699 kb on - strand at 1054.830 kb on - strand at 1054.830 kb on - strand at 1054.830 kb on - strand at 1054.830 kb on - strand at 1054.830 kb on - strand at 1054.863 kb on - strand at 1054.863 kb on - strand at 1054.880 kb on - strand at 1055.461 kb on + strand, within RR42_RS04740 at 1055.461 kb on + strand, within RR42_RS04740 at 1055.461 kb on + strand, within RR42_RS04740 at 1055.461 kb on + strand, within RR42_RS04740 at 1055.462 kb on - strand, within RR42_RS04740 at 1055.497 kb on + strand, within RR42_RS04740 at 1055.684 kb on - strand, within RR42_RS04740 at 1055.684 kb on - strand, within RR42_RS04740 at 1055.816 kb on + strand, within RR42_RS04740 at 1055.873 kb on - strand, within RR42_RS04740 at 1056.202 kb on + strand, within RR42_RS04740 at 1056.202 kb on + strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.461 kb on + strand at 1056.532 kb on + strand at 1056.532 kb on + strand at 1056.533 kb on - strand at 1056.533 kb on - strand at 1056.533 kb on - strand at 1056.552 kb on + strand at 1056.553 kb on - strand at 1056.868 kb on + strand at 1056.869 kb on - strand at 1056.927 kb on + strand at 1056.967 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.029 kb on - strand at 1057.029 kb on - strand at 1057.029 kb on - strand at 1057.099 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.124 kb on - strand, within RR42_RS04745 at 1057.140 kb on + strand, within RR42_RS04745 at 1057.141 kb on - strand, within RR42_RS04745 at 1057.199 kb on + strand, within RR42_RS04745 at 1057.200 kb on - strand, within RR42_RS04745 at 1057.230 kb on + strand, within RR42_RS04745 at 1057.231 kb on - strand, within RR42_RS04745 at 1057.231 kb on - strand, within RR42_RS04745 at 1057.268 kb on + strand, within RR42_RS04745 at 1057.268 kb on + strand, within RR42_RS04745 at 1057.269 kb on - strand, within RR42_RS04745 at 1057.269 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.482 kb on - strand, within RR42_RS04745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,054,482 - RR42_RS04735 0.66 -0.8 1,054,699 - -1.7 1,054,830 - -0.2 1,054,830 - -0.9 1,054,830 - -1.1 1,054,830 - +0.1 1,054,830 - -0.6 1,054,863 - +1.8 1,054,863 - +0.0 1,054,880 - +2.0 1,055,461 + RR42_RS04740 0.31 -0.6 1,055,461 + RR42_RS04740 0.31 +1.0 1,055,461 + RR42_RS04740 0.31 +1.0 1,055,461 + RR42_RS04740 0.31 +0.5 1,055,462 - RR42_RS04740 0.31 -1.4 1,055,497 + RR42_RS04740 0.33 +0.6 1,055,684 - RR42_RS04740 0.45 +2.6 1,055,684 - RR42_RS04740 0.45 -1.5 1,055,816 + RR42_RS04740 0.54 -0.7 1,055,873 - RR42_RS04740 0.57 +0.6 1,056,202 + RR42_RS04740 0.79 -0.2 1,056,202 + RR42_RS04740 0.79 +0.4 1,056,203 - RR42_RS04740 0.79 -2.3 1,056,203 - RR42_RS04740 0.79 -0.4 1,056,203 - RR42_RS04740 0.79 -0.6 1,056,203 - RR42_RS04740 0.79 +0.3 1,056,203 - RR42_RS04740 0.79 -2.1 1,056,203 - RR42_RS04740 0.79 -2.8 1,056,203 - RR42_RS04740 0.79 -1.3 1,056,461 + -0.3 1,056,532 + +1.6 1,056,532 + +0.4 1,056,533 - -2.1 1,056,533 - -0.7 1,056,533 - -0.6 1,056,552 + -0.1 1,056,553 - -0.1 1,056,868 + +1.6 1,056,869 - +1.0 1,056,927 + -1.3 1,056,967 + -0.3 1,057,028 + +2.8 1,057,028 + +1.6 1,057,028 + -0.3 1,057,028 + +0.3 1,057,028 + +1.2 1,057,028 + -0.8 1,057,028 + -0.2 1,057,028 + +1.1 1,057,029 - -0.0 1,057,029 - +0.7 1,057,029 - +0.6 1,057,099 + RR42_RS04745 0.19 -1.0 1,057,123 + RR42_RS04745 0.23 -0.1 1,057,123 + RR42_RS04745 0.23 -1.8 1,057,123 + RR42_RS04745 0.23 +0.1 1,057,123 + RR42_RS04745 0.23 +0.2 1,057,123 + RR42_RS04745 0.23 -0.8 1,057,124 - RR42_RS04745 0.23 +1.8 1,057,140 + RR42_RS04745 0.26 -0.4 1,057,141 - RR42_RS04745 0.26 +0.7 1,057,199 + RR42_RS04745 0.35 +0.2 1,057,200 - RR42_RS04745 0.35 +0.1 1,057,230 + RR42_RS04745 0.39 +0.0 1,057,231 - RR42_RS04745 0.40 +0.6 1,057,231 - RR42_RS04745 0.40 -0.2 1,057,268 + RR42_RS04745 0.45 -2.0 1,057,268 + RR42_RS04745 0.45 +1.0 1,057,269 - RR42_RS04745 0.45 +0.4 1,057,269 - RR42_RS04745 0.45 -0.5 1,057,309 - RR42_RS04745 0.52 -1.0 1,057,309 - RR42_RS04745 0.52 -2.1 1,057,309 - RR42_RS04745 0.52 -1.7 1,057,309 - RR42_RS04745 0.52 -0.1 1,057,482 - RR42_RS04745 0.78 -1.3
Or see this region's nucleotide sequence