Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04690 and RR42_RS04695 are separated by 137 nucleotides RR42_RS04695 and RR42_RS04700 are separated by 12 nucleotides RR42_RS04700 and RR42_RS04705 are separated by 132 nucleotides RR42_RS04705 and RR42_RS04710 are separated by 108 nucleotides
RR42_RS04690: RR42_RS04690 - fatty acid hydroxylase, at 1,045,445 to 1,046,365
_RS04690
RR42_RS04695: RR42_RS04695 - hypothetical protein, at 1,046,503 to 1,047,117
_RS04695
RR42_RS04700: RR42_RS04700 - ribonuclease G, at 1,047,130 to 1,048,596
_RS04700
RR42_RS04705: RR42_RS04705 - septum formation inhibitor Maf, at 1,048,729 to 1,049,334
_RS04705
RR42_RS04710: RR42_RS04710 - 50S rRNA methyltransferase, at 1,049,443 to 1,049,913
_RS04710
Position (kb)
1047
1048
1049 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1046.234 kb on + strand, within RR42_RS04690 at 1046.235 kb on - strand, within RR42_RS04690 at 1046.337 kb on + strand at 1046.337 kb on + strand at 1046.337 kb on + strand at 1046.338 kb on - strand at 1046.338 kb on - strand at 1046.338 kb on - strand at 1046.394 kb on + strand at 1046.394 kb on + strand at 1046.394 kb on + strand at 1046.395 kb on - strand at 1046.395 kb on - strand at 1046.395 kb on - strand at 1046.406 kb on + strand at 1046.406 kb on + strand at 1046.407 kb on - strand at 1046.407 kb on - strand at 1046.407 kb on - strand at 1046.408 kb on + strand at 1046.409 kb on - strand at 1046.409 kb on - strand at 1046.409 kb on - strand at 1046.409 kb on - strand at 1046.409 kb on - strand at 1046.409 kb on - strand at 1046.415 kb on + strand at 1046.415 kb on + strand at 1046.416 kb on - strand at 1046.416 kb on - strand at 1046.432 kb on - strand at 1046.537 kb on + strand at 1046.537 kb on + strand at 1046.537 kb on + strand at 1046.537 kb on + strand at 1046.538 kb on - strand at 1046.636 kb on - strand, within RR42_RS04695 at 1046.641 kb on + strand, within RR42_RS04695 at 1046.765 kb on + strand, within RR42_RS04695 at 1046.766 kb on - strand, within RR42_RS04695 at 1046.783 kb on + strand, within RR42_RS04695 at 1046.783 kb on + strand, within RR42_RS04695 at 1046.831 kb on + strand, within RR42_RS04695 at 1046.832 kb on - strand, within RR42_RS04695 at 1046.832 kb on - strand, within RR42_RS04695 at 1047.971 kb on + strand, within RR42_RS04700 at 1048.313 kb on + strand, within RR42_RS04700 at 1048.472 kb on + strand at 1048.472 kb on + strand at 1048.473 kb on - strand at 1048.597 kb on - strand at 1048.800 kb on - strand, within RR42_RS04705 at 1048.850 kb on + strand, within RR42_RS04705 at 1048.889 kb on + strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1049.180 kb on + strand, within RR42_RS04705 at 1049.259 kb on - strand, within RR42_RS04705 at 1049.259 kb on - strand, within RR42_RS04705 at 1049.315 kb on + strand at 1049.316 kb on - strand at 1049.316 kb on - strand at 1049.325 kb on + strand at 1049.325 kb on + strand at 1049.325 kb on + strand at 1049.326 kb on - strand at 1049.351 kb on - strand at 1049.400 kb on + strand at 1049.400 kb on + strand at 1049.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 1,046,234 + RR42_RS04690 0.86 -1.8 1,046,235 - RR42_RS04690 0.86 +0.4 1,046,337 + -0.8 1,046,337 + -1.3 1,046,337 + +0.4 1,046,338 - -0.5 1,046,338 - -1.6 1,046,338 - +0.5 1,046,394 + +0.8 1,046,394 + +0.0 1,046,394 + +1.4 1,046,395 - +0.2 1,046,395 - +0.7 1,046,395 - +0.3 1,046,406 + -0.7 1,046,406 + -1.1 1,046,407 - +0.3 1,046,407 - +0.7 1,046,407 - +0.5 1,046,408 + -0.8 1,046,409 - -1.0 1,046,409 - +1.5 1,046,409 - -1.3 1,046,409 - +0.0 1,046,409 - -3.2 1,046,409 - +2.2 1,046,415 + +0.2 1,046,415 + -0.7 1,046,416 - -0.6 1,046,416 - -2.0 1,046,432 - +0.0 1,046,537 + +1.8 1,046,537 + +0.8 1,046,537 + -1.0 1,046,537 + -0.8 1,046,538 - -0.1 1,046,636 - RR42_RS04695 0.22 -0.6 1,046,641 + RR42_RS04695 0.22 -1.1 1,046,765 + RR42_RS04695 0.43 +2.8 1,046,766 - RR42_RS04695 0.43 -0.3 1,046,783 + RR42_RS04695 0.46 +0.8 1,046,783 + RR42_RS04695 0.46 -1.4 1,046,831 + RR42_RS04695 0.53 -0.8 1,046,832 - RR42_RS04695 0.53 +0.4 1,046,832 - RR42_RS04695 0.53 +0.3 1,047,971 + RR42_RS04700 0.57 -2.1 1,048,313 + RR42_RS04700 0.81 -3.1 1,048,472 + +1.0 1,048,472 + +0.3 1,048,473 - -0.6 1,048,597 - -2.4 1,048,800 - RR42_RS04705 0.12 +2.2 1,048,850 + RR42_RS04705 0.20 +0.7 1,048,889 + RR42_RS04705 0.26 -0.3 1,048,890 - RR42_RS04705 0.27 +0.2 1,048,890 - RR42_RS04705 0.27 -0.6 1,048,890 - RR42_RS04705 0.27 +0.2 1,048,890 - RR42_RS04705 0.27 -0.1 1,049,180 + RR42_RS04705 0.74 +1.0 1,049,259 - RR42_RS04705 0.87 -2.6 1,049,259 - RR42_RS04705 0.87 -0.7 1,049,315 + -1.0 1,049,316 - -1.6 1,049,316 - -1.0 1,049,325 + +0.3 1,049,325 + -0.1 1,049,325 + -1.3 1,049,326 - +1.2 1,049,351 - +0.3 1,049,400 + -0.3 1,049,400 + -0.1 1,049,457 - -1.0
Or see this region's nucleotide sequence