Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04345 and RR42_RS04350 are separated by 243 nucleotides RR42_RS04350 and RR42_RS04355 are separated by 116 nucleotides
RR42_RS04345: RR42_RS04345 - iron ABC transporter permease, at 964,983 to 966,611
_RS04345
RR42_RS04350: RR42_RS04350 - ABC transporter ATP-binding protein, at 966,855 to 967,916
_RS04350
RR42_RS04355: RR42_RS04355 - recombination factor protein RarA, at 968,033 to 969,400
_RS04355
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 965.947 kb on + strand, within RR42_RS04345 at 965.980 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.308 kb on + strand, within RR42_RS04345 at 966.311 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.467 kb on + strand at 966.467 kb on + strand at 966.467 kb on + strand at 966.468 kb on - strand at 966.478 kb on + strand at 966.479 kb on - strand at 966.657 kb on + strand at 966.657 kb on + strand at 966.658 kb on - strand at 966.713 kb on - strand at 966.751 kb on - strand at 966.762 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.813 kb on - strand at 966.813 kb on - strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.815 kb on - strand at 966.815 kb on - strand at 967.132 kb on - strand, within RR42_RS04350 at 967.132 kb on - strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.539 kb on + strand, within RR42_RS04350 at 967.649 kb on + strand, within RR42_RS04350 at 967.649 kb on + strand, within RR42_RS04350 at 967.650 kb on - strand, within RR42_RS04350 at 967.650 kb on - strand, within RR42_RS04350 at 967.789 kb on - strand, within RR42_RS04350 at 967.949 kb on + strand at 967.949 kb on + strand at 967.950 kb on - strand at 967.950 kb on - strand at 967.950 kb on - strand at 968.005 kb on + strand at 968.006 kb on - strand at 968.017 kb on + strand at 968.325 kb on - strand, within RR42_RS04355 at 968.502 kb on - strand, within RR42_RS04355 at 968.502 kb on - strand, within RR42_RS04355 at 968.765 kb on + strand, within RR42_RS04355 at 968.765 kb on + strand, within RR42_RS04355 at 968.825 kb on + strand, within RR42_RS04355 at 968.825 kb on + strand, within RR42_RS04355 at 968.826 kb on - strand, within RR42_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 965,947 + RR42_RS04345 0.59 -2.4 965,980 + RR42_RS04345 0.61 +0.1 966,169 + RR42_RS04345 0.73 -2.5 966,169 + RR42_RS04345 0.73 -1.2 966,308 + RR42_RS04345 0.81 -0.5 966,311 - RR42_RS04345 0.82 -1.1 966,329 - RR42_RS04345 0.83 -1.5 966,329 - RR42_RS04345 0.83 -0.9 966,391 + RR42_RS04345 0.86 -1.7 966,391 + RR42_RS04345 0.86 -0.4 966,467 + -0.9 966,467 + -1.3 966,467 + -0.7 966,468 - -0.6 966,478 + +0.7 966,479 - +0.8 966,657 + -0.3 966,657 + +0.7 966,658 - -0.3 966,713 - -1.9 966,751 - -0.1 966,762 + -0.0 966,812 + +0.2 966,812 + +1.1 966,812 + -0.4 966,812 + +4.2 966,812 + -1.1 966,813 - +0.4 966,813 - +1.2 966,814 + +0.3 966,814 + +1.1 966,814 + +0.3 966,815 - -1.6 966,815 - +1.6 967,132 - RR42_RS04350 0.26 +1.1 967,132 - RR42_RS04350 0.26 +1.9 967,532 + RR42_RS04350 0.64 +1.2 967,532 + RR42_RS04350 0.64 -1.3 967,539 + RR42_RS04350 0.64 +1.8 967,649 + RR42_RS04350 0.75 +0.8 967,649 + RR42_RS04350 0.75 -0.5 967,650 - RR42_RS04350 0.75 +2.1 967,650 - RR42_RS04350 0.75 +0.6 967,789 - RR42_RS04350 0.88 -1.5 967,949 + -2.3 967,949 + -0.0 967,950 - +1.5 967,950 - +0.5 967,950 - +0.1 968,005 + +1.1 968,006 - +1.1 968,017 + +0.9 968,325 - RR42_RS04355 0.21 +0.1 968,502 - RR42_RS04355 0.34 +0.2 968,502 - RR42_RS04355 0.34 +0.1 968,765 + RR42_RS04355 0.54 +0.3 968,765 + RR42_RS04355 0.54 -2.3 968,825 + RR42_RS04355 0.58 -1.3 968,825 + RR42_RS04355 0.58 -1.7 968,826 - RR42_RS04355 0.58 -0.6
Or see this region's nucleotide sequence