Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04335 and RR42_RS04340 are separated by 201 nucleotides RR42_RS04340 and RR42_RS04345 are separated by 208 nucleotides
RR42_RS04335: RR42_RS04335 - iron deficiency-induced protein A, at 962,849 to 963,892
_RS04335
RR42_RS04340: RR42_RS04340 - hypothetical protein, at 964,094 to 964,774
_RS04340
RR42_RS04345: RR42_RS04345 - iron ABC transporter permease, at 964,983 to 966,611
_RS04345
Position (kb)
964
965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 963.200 kb on + strand, within RR42_RS04335 at 963.200 kb on + strand, within RR42_RS04335 at 963.257 kb on + strand, within RR42_RS04335 at 963.258 kb on - strand, within RR42_RS04335 at 963.259 kb on + strand, within RR42_RS04335 at 963.260 kb on - strand, within RR42_RS04335 at 963.260 kb on - strand, within RR42_RS04335 at 963.296 kb on + strand, within RR42_RS04335 at 963.360 kb on - strand, within RR42_RS04335 at 963.527 kb on + strand, within RR42_RS04335 at 963.527 kb on + strand, within RR42_RS04335 at 963.528 kb on - strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.699 kb on - strand, within RR42_RS04335 at 963.726 kb on - strand, within RR42_RS04335 at 963.884 kb on + strand at 963.884 kb on + strand at 963.885 kb on - strand at 963.885 kb on - strand at 963.891 kb on - strand at 963.891 kb on - strand at 963.891 kb on - strand at 964.075 kb on + strand at 964.096 kb on - strand at 964.275 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.347 kb on - strand, within RR42_RS04340 at 964.401 kb on + strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on - strand at 964.922 kb on + strand at 964.923 kb on - strand at 964.985 kb on - strand at 965.113 kb on + strand at 965.114 kb on - strand at 965.114 kb on - strand at 965.366 kb on - strand, within RR42_RS04345 at 965.372 kb on - strand, within RR42_RS04345 at 965.512 kb on + strand, within RR42_RS04345 at 965.646 kb on - strand, within RR42_RS04345 at 965.761 kb on - strand, within RR42_RS04345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 963,200 + RR42_RS04335 0.34 +0.7 963,200 + RR42_RS04335 0.34 -0.7 963,257 + RR42_RS04335 0.39 -1.2 963,258 - RR42_RS04335 0.39 -1.9 963,259 + RR42_RS04335 0.39 +0.7 963,260 - RR42_RS04335 0.39 -0.5 963,260 - RR42_RS04335 0.39 -0.4 963,296 + RR42_RS04335 0.43 -0.9 963,360 - RR42_RS04335 0.49 +2.8 963,527 + RR42_RS04335 0.65 -0.7 963,527 + RR42_RS04335 0.65 -0.5 963,528 - RR42_RS04335 0.65 -1.9 963,530 + RR42_RS04335 0.65 +1.1 963,530 + RR42_RS04335 0.65 -0.7 963,530 + RR42_RS04335 0.65 +0.4 963,530 + RR42_RS04335 0.65 +0.1 963,531 - RR42_RS04335 0.65 -0.7 963,531 - RR42_RS04335 0.65 +2.9 963,531 - RR42_RS04335 0.65 -0.2 963,699 - RR42_RS04335 0.81 -0.3 963,726 - RR42_RS04335 0.84 +3.4 963,884 + -0.9 963,884 + +0.1 963,885 - +2.3 963,885 - +0.7 963,891 - +0.9 963,891 - -1.9 963,891 - -0.3 964,075 + -2.6 964,096 - +0.8 964,275 + RR42_RS04340 0.27 -0.7 964,346 + RR42_RS04340 0.37 -0.4 964,346 + RR42_RS04340 0.37 +1.4 964,347 - RR42_RS04340 0.37 -0.6 964,401 + RR42_RS04340 0.45 -1.3 964,402 - RR42_RS04340 0.45 +0.9 964,402 - RR42_RS04340 0.45 +0.5 964,546 + RR42_RS04340 0.66 -2.5 964,546 + RR42_RS04340 0.66 +0.1 964,546 + RR42_RS04340 0.66 +1.4 964,546 + RR42_RS04340 0.66 -2.5 964,546 + RR42_RS04340 0.66 +0.2 964,546 + RR42_RS04340 0.66 -0.0 964,774 + +0.6 964,774 + -2.5 964,774 + -1.7 964,774 + -0.4 964,774 + -0.2 964,774 - -1.1 964,922 + +0.4 964,923 - -0.4 964,985 - +1.1 965,113 + +0.5 965,114 - -2.8 965,114 - -1.2 965,366 - RR42_RS04345 0.24 +0.5 965,372 - RR42_RS04345 0.24 -1.4 965,512 + RR42_RS04345 0.32 +1.4 965,646 - RR42_RS04345 0.41 -0.3 965,761 - RR42_RS04345 0.48 -0.5
Or see this region's nucleotide sequence