Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04325 and RR42_RS04330 are separated by 23 nucleotides RR42_RS04330 and RR42_RS04335 are separated by 236 nucleotides RR42_RS04335 and RR42_RS04340 are separated by 201 nucleotides
RR42_RS04325: RR42_RS04325 - membrane protein, at 961,546 to 962,184
_RS04325
RR42_RS04330: RR42_RS04330 - lipoprotein, at 962,208 to 962,612
_RS04330
RR42_RS04335: RR42_RS04335 - iron deficiency-induced protein A, at 962,849 to 963,892
_RS04335
RR42_RS04340: RR42_RS04340 - hypothetical protein, at 964,094 to 964,774
_RS04340
Position (kb)
962
963
964 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 962.657 kb on + strand at 962.703 kb on - strand at 962.703 kb on - strand at 962.717 kb on - strand at 962.777 kb on + strand at 962.823 kb on + strand at 962.823 kb on + strand at 962.823 kb on + strand at 962.857 kb on - strand at 962.857 kb on - strand at 962.954 kb on + strand, within RR42_RS04335 at 962.970 kb on - strand, within RR42_RS04335 at 962.990 kb on + strand, within RR42_RS04335 at 962.991 kb on - strand, within RR42_RS04335 at 963.200 kb on + strand, within RR42_RS04335 at 963.200 kb on + strand, within RR42_RS04335 at 963.257 kb on + strand, within RR42_RS04335 at 963.258 kb on - strand, within RR42_RS04335 at 963.259 kb on + strand, within RR42_RS04335 at 963.260 kb on - strand, within RR42_RS04335 at 963.260 kb on - strand, within RR42_RS04335 at 963.296 kb on + strand, within RR42_RS04335 at 963.360 kb on - strand, within RR42_RS04335 at 963.527 kb on + strand, within RR42_RS04335 at 963.527 kb on + strand, within RR42_RS04335 at 963.528 kb on - strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.530 kb on + strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.531 kb on - strand, within RR42_RS04335 at 963.699 kb on - strand, within RR42_RS04335 at 963.726 kb on - strand, within RR42_RS04335 at 963.884 kb on + strand at 963.884 kb on + strand at 963.885 kb on - strand at 963.885 kb on - strand at 963.891 kb on - strand at 963.891 kb on - strand at 963.891 kb on - strand at 964.075 kb on + strand at 964.096 kb on - strand at 964.275 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.347 kb on - strand, within RR42_RS04340 at 964.401 kb on + strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 962,657 + -0.2 962,703 - -0.5 962,703 - +0.2 962,717 - +0.1 962,777 + -0.0 962,823 + +1.8 962,823 + -1.9 962,823 + -0.0 962,857 - +0.2 962,857 - +0.6 962,954 + RR42_RS04335 0.10 -0.1 962,970 - RR42_RS04335 0.12 +1.0 962,990 + RR42_RS04335 0.14 -0.3 962,991 - RR42_RS04335 0.14 +1.0 963,200 + RR42_RS04335 0.34 +0.7 963,200 + RR42_RS04335 0.34 -0.7 963,257 + RR42_RS04335 0.39 -1.2 963,258 - RR42_RS04335 0.39 -1.9 963,259 + RR42_RS04335 0.39 +0.7 963,260 - RR42_RS04335 0.39 -0.5 963,260 - RR42_RS04335 0.39 -0.4 963,296 + RR42_RS04335 0.43 -0.9 963,360 - RR42_RS04335 0.49 +2.8 963,527 + RR42_RS04335 0.65 -0.7 963,527 + RR42_RS04335 0.65 -0.5 963,528 - RR42_RS04335 0.65 -1.9 963,530 + RR42_RS04335 0.65 +1.1 963,530 + RR42_RS04335 0.65 -0.7 963,530 + RR42_RS04335 0.65 +0.4 963,530 + RR42_RS04335 0.65 +0.1 963,531 - RR42_RS04335 0.65 -0.7 963,531 - RR42_RS04335 0.65 +2.9 963,531 - RR42_RS04335 0.65 -0.2 963,699 - RR42_RS04335 0.81 -0.3 963,726 - RR42_RS04335 0.84 +3.4 963,884 + -0.9 963,884 + +0.1 963,885 - +2.3 963,885 - +0.7 963,891 - +0.9 963,891 - -1.9 963,891 - -0.3 964,075 + -2.6 964,096 - +0.8 964,275 + RR42_RS04340 0.27 -0.7 964,346 + RR42_RS04340 0.37 -0.4 964,346 + RR42_RS04340 0.37 +1.4 964,347 - RR42_RS04340 0.37 -0.6 964,401 + RR42_RS04340 0.45 -1.3 964,402 - RR42_RS04340 0.45 +0.9 964,402 - RR42_RS04340 0.45 +0.5 964,546 + RR42_RS04340 0.66 -2.5 964,546 + RR42_RS04340 0.66 +0.1 964,546 + RR42_RS04340 0.66 +1.4 964,546 + RR42_RS04340 0.66 -2.5 964,546 + RR42_RS04340 0.66 +0.2 964,546 + RR42_RS04340 0.66 -0.0 964,774 + +0.6 964,774 + -2.5 964,774 + -1.7 964,774 + -0.4 964,774 + -0.2 964,774 - -1.1
Or see this region's nucleotide sequence