Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04260 and RR42_RS04265 are separated by 85 nucleotides RR42_RS04265 and RR42_RS04270 are separated by 476 nucleotides
RR42_RS04260: RR42_RS04260 - chemotaxis protein, at 941,179 to 943,347
_RS04260
RR42_RS04265: RR42_RS04265 - chemotaxis protein CheY, at 943,433 to 949,480
_RS04265
RR42_RS04270: RR42_RS04270 - MFS transporter, at 949,957 to 951,261
_RS04270
Position (kb) 943
944
945
946
947
948
949
950 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 942.478 kb on + strand, within RR42_RS04260 at 942.478 kb on + strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 943.200 kb on + strand at 943.218 kb on + strand at 943.219 kb on - strand at 943.317 kb on + strand at 943.756 kb on - strand at 943.782 kb on - strand at 943.889 kb on + strand at 943.890 kb on - strand at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.185 kb on - strand, within RR42_RS04265 at 944.397 kb on - strand, within RR42_RS04265 at 944.547 kb on - strand, within RR42_RS04265 at 944.549 kb on + strand, within RR42_RS04265 at 944.549 kb on + strand, within RR42_RS04265 at 944.550 kb on - strand, within RR42_RS04265 at 944.550 kb on - strand, within RR42_RS04265 at 944.648 kb on - strand, within RR42_RS04265 at 944.876 kb on + strand, within RR42_RS04265 at 944.876 kb on + strand, within RR42_RS04265 at 944.877 kb on - strand, within RR42_RS04265 at 944.877 kb on - strand, within RR42_RS04265 at 944.877 kb on - strand, within RR42_RS04265 at 945.029 kb on + strand, within RR42_RS04265 at 945.030 kb on - strand, within RR42_RS04265 at 945.370 kb on + strand, within RR42_RS04265 at 945.370 kb on + strand, within RR42_RS04265 at 945.371 kb on - strand, within RR42_RS04265 at 945.371 kb on - strand, within RR42_RS04265 at 945.371 kb on - strand, within RR42_RS04265 at 945.371 kb on - strand, within RR42_RS04265 at 945.598 kb on + strand, within RR42_RS04265 at 945.599 kb on - strand, within RR42_RS04265 at 945.760 kb on + strand, within RR42_RS04265 at 945.875 kb on - strand, within RR42_RS04265 at 945.875 kb on - strand, within RR42_RS04265 at 946.403 kb on + strand, within RR42_RS04265 at 946.404 kb on - strand, within RR42_RS04265 at 946.413 kb on - strand, within RR42_RS04265 at 946.937 kb on - strand, within RR42_RS04265 at 947.246 kb on + strand, within RR42_RS04265 at 947.275 kb on + strand, within RR42_RS04265 at 947.276 kb on - strand, within RR42_RS04265 at 947.276 kb on - strand, within RR42_RS04265 at 947.439 kb on + strand, within RR42_RS04265 at 947.439 kb on + strand, within RR42_RS04265 at 947.440 kb on - strand, within RR42_RS04265 at 947.581 kb on + strand, within RR42_RS04265 at 947.658 kb on - strand, within RR42_RS04265 at 947.993 kb on + strand, within RR42_RS04265 at 948.036 kb on + strand, within RR42_RS04265 at 948.133 kb on - strand, within RR42_RS04265 at 948.520 kb on + strand, within RR42_RS04265 at 948.521 kb on - strand, within RR42_RS04265 at 948.624 kb on - strand, within RR42_RS04265 at 948.722 kb on + strand, within RR42_RS04265 at 948.722 kb on + strand, within RR42_RS04265 at 948.723 kb on - strand, within RR42_RS04265 at 948.890 kb on + strand at 948.891 kb on - strand at 948.891 kb on - strand at 949.228 kb on + strand at 949.228 kb on + strand at 949.229 kb on - strand at 949.229 kb on - strand at 949.229 kb on - strand at 949.282 kb on + strand at 949.282 kb on + strand at 949.282 kb on + strand at 949.359 kb on - strand at 949.373 kb on + strand at 949.373 kb on + strand at 949.373 kb on + strand at 949.373 kb on + strand at 949.773 kb on + strand at 949.773 kb on + strand at 949.773 kb on + strand at 949.959 kb on - strand at 950.031 kb on - strand at 950.243 kb on + strand, within RR42_RS04270 at 950.244 kb on + strand, within RR42_RS04270 at 950.244 kb on + strand, within RR42_RS04270 at 950.245 kb on - strand, within RR42_RS04270 at 950.343 kb on - strand, within RR42_RS04270 at 950.379 kb on - strand, within RR42_RS04270 at 950.465 kb on + strand, within RR42_RS04270 at 950.466 kb on - strand, within RR42_RS04270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 942,478 + RR42_RS04260 0.60 +0.7 942,478 + RR42_RS04260 0.60 -1.3 942,479 - RR42_RS04260 0.60 -3.2 942,479 - RR42_RS04260 0.60 -0.4 942,479 - RR42_RS04260 0.60 -0.5 942,479 - RR42_RS04260 0.60 -1.5 943,200 + -2.1 943,218 + -0.1 943,219 - +0.7 943,317 + +0.6 943,756 - -0.3 943,782 - -0.9 943,889 + -1.3 943,890 - -1.5 944,153 + RR42_RS04265 0.12 -0.8 944,153 + RR42_RS04265 0.12 -1.9 944,153 + RR42_RS04265 0.12 -0.1 944,153 + RR42_RS04265 0.12 +0.1 944,185 - RR42_RS04265 0.12 -1.5 944,397 - RR42_RS04265 0.16 -1.0 944,547 - RR42_RS04265 0.18 -1.8 944,549 + RR42_RS04265 0.18 -0.0 944,549 + RR42_RS04265 0.18 -1.0 944,550 - RR42_RS04265 0.18 -0.4 944,550 - RR42_RS04265 0.18 -0.2 944,648 - RR42_RS04265 0.20 -2.8 944,876 + RR42_RS04265 0.24 -0.2 944,876 + RR42_RS04265 0.24 -0.5 944,877 - RR42_RS04265 0.24 +0.1 944,877 - RR42_RS04265 0.24 -2.1 944,877 - RR42_RS04265 0.24 -2.0 945,029 + RR42_RS04265 0.26 +0.5 945,030 - RR42_RS04265 0.26 +1.2 945,370 + RR42_RS04265 0.32 -1.3 945,370 + RR42_RS04265 0.32 -1.0 945,371 - RR42_RS04265 0.32 -1.5 945,371 - RR42_RS04265 0.32 -0.3 945,371 - RR42_RS04265 0.32 -2.1 945,371 - RR42_RS04265 0.32 -3.8 945,598 + RR42_RS04265 0.36 -1.5 945,599 - RR42_RS04265 0.36 +0.8 945,760 + RR42_RS04265 0.38 -0.5 945,875 - RR42_RS04265 0.40 +2.1 945,875 - RR42_RS04265 0.40 -0.6 946,403 + RR42_RS04265 0.49 -4.2 946,404 - RR42_RS04265 0.49 -0.2 946,413 - RR42_RS04265 0.49 +1.9 946,937 - RR42_RS04265 0.58 +0.3 947,246 + RR42_RS04265 0.63 +0.8 947,275 + RR42_RS04265 0.64 -0.7 947,276 - RR42_RS04265 0.64 -2.9 947,276 - RR42_RS04265 0.64 -0.2 947,439 + RR42_RS04265 0.66 +0.6 947,439 + RR42_RS04265 0.66 +0.8 947,440 - RR42_RS04265 0.66 -0.5 947,581 + RR42_RS04265 0.69 +0.0 947,658 - RR42_RS04265 0.70 -0.4 947,993 + RR42_RS04265 0.75 +0.9 948,036 + RR42_RS04265 0.76 +2.1 948,133 - RR42_RS04265 0.78 -2.1 948,520 + RR42_RS04265 0.84 -0.4 948,521 - RR42_RS04265 0.84 +0.3 948,624 - RR42_RS04265 0.86 -0.2 948,722 + RR42_RS04265 0.87 -3.2 948,722 + RR42_RS04265 0.87 -0.2 948,723 - RR42_RS04265 0.87 -0.9 948,890 + +0.2 948,891 - +0.6 948,891 - -1.0 949,228 + +0.8 949,228 + -1.7 949,229 - -1.7 949,229 - +0.5 949,229 - +1.1 949,282 + +0.1 949,282 + -2.2 949,282 + +0.2 949,359 - +1.6 949,373 + -0.9 949,373 + -1.1 949,373 + -0.6 949,373 + -0.3 949,773 + -1.7 949,773 + -1.7 949,773 + +0.2 949,959 - -0.4 950,031 - -2.7 950,243 + RR42_RS04270 0.22 -0.7 950,244 + RR42_RS04270 0.22 -0.2 950,244 + RR42_RS04270 0.22 +0.7 950,245 - RR42_RS04270 0.22 +0.4 950,343 - RR42_RS04270 0.30 +2.6 950,379 - RR42_RS04270 0.32 -2.0 950,465 + RR42_RS04270 0.39 +0.3 950,466 - RR42_RS04270 0.39 +1.6
Or see this region's nucleotide sequence