Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04215 and RR42_RS04220 are separated by 6 nucleotides RR42_RS04220 and RR42_RS04225 are separated by 15 nucleotides RR42_RS04225 and RR42_RS04230 are separated by 426 nucleotides
RR42_RS04215: RR42_RS04215 - hypothetical protein, at 933,296 to 933,799
_RS04215
RR42_RS04220: RR42_RS04220 - membrane protein, at 933,806 to 934,666
_RS04220
RR42_RS04225: RR42_RS04225 - hypothetical protein, at 934,682 to 935,302
_RS04225
RR42_RS04230: RR42_RS04230 - dihydrodipicolinate synthase, at 935,729 to 936,637
_RS04230
Position (kb) 934
935
936 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 934.185 kb on + strand, within RR42_RS04220 at 934.186 kb on - strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.424 kb on + strand, within RR42_RS04220 at 934.464 kb on + strand, within RR42_RS04220 at 934.610 kb on + strand at 934.666 kb on + strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.806 kb on + strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.826 kb on - strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 935.091 kb on + strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.105 kb on - strand, within RR42_RS04225 at 935.143 kb on - strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.258 kb on + strand at 935.258 kb on + strand at 935.335 kb on + strand at 935.336 kb on - strand at 935.336 kb on - strand at 935.362 kb on + strand at 935.363 kb on - strand at 935.613 kb on + strand at 935.613 kb on + strand at 935.614 kb on - strand at 935.614 kb on - strand at 935.614 kb on - strand at 935.714 kb on + strand at 935.714 kb on + strand at 935.739 kb on + strand at 935.740 kb on - strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.749 kb on - strand at 935.749 kb on - strand at 935.843 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.248 kb on + strand, within RR42_RS04230 at 936.249 kb on - strand, within RR42_RS04230 at 936.249 kb on - strand, within RR42_RS04230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 934,185 + RR42_RS04220 0.44 +1.5 934,186 - RR42_RS04220 0.44 +0.2 934,392 + RR42_RS04220 0.68 +0.2 934,392 + RR42_RS04220 0.68 +0.6 934,424 + RR42_RS04220 0.72 -1.4 934,464 + RR42_RS04220 0.76 -0.5 934,610 + -0.5 934,666 + +0.2 934,667 - -1.1 934,667 - -1.4 934,667 - +1.0 934,667 - -0.2 934,670 + -0.9 934,670 + -0.5 934,670 + -0.6 934,670 + -1.6 934,671 - +0.6 934,671 - -2.3 934,671 - +0.4 934,671 - +0.4 934,671 - +1.3 934,671 - +1.0 934,806 + RR42_RS04225 0.20 +0.3 934,807 - RR42_RS04225 0.20 -0.9 934,807 - RR42_RS04225 0.20 -2.1 934,826 - RR42_RS04225 0.23 +0.7 934,827 + RR42_RS04225 0.23 +0.5 934,827 + RR42_RS04225 0.23 +1.6 934,828 - RR42_RS04225 0.24 -1.7 934,828 - RR42_RS04225 0.24 -1.3 934,828 - RR42_RS04225 0.24 +1.1 934,828 - RR42_RS04225 0.24 +0.7 935,091 + RR42_RS04225 0.66 +0.8 935,092 - RR42_RS04225 0.66 +0.5 935,092 - RR42_RS04225 0.66 +0.7 935,105 - RR42_RS04225 0.68 +0.2 935,143 - RR42_RS04225 0.74 +0.1 935,205 + RR42_RS04225 0.84 +0.1 935,205 + RR42_RS04225 0.84 -3.0 935,258 + +0.1 935,258 + +1.6 935,335 + -0.4 935,336 - -0.5 935,336 - +0.1 935,362 + -1.5 935,363 - +0.2 935,613 + +0.9 935,613 + +1.0 935,614 - -2.1 935,614 - -2.7 935,614 - -0.7 935,714 + -0.8 935,714 + +0.7 935,739 + -1.1 935,740 - +0.2 935,748 + +0.1 935,748 + -0.4 935,748 + -0.9 935,748 + -1.3 935,749 - -2.1 935,749 - +0.3 935,843 + RR42_RS04230 0.13 +0.3 936,068 + RR42_RS04230 0.37 -1.3 936,068 + RR42_RS04230 0.37 -0.0 936,068 + RR42_RS04230 0.37 -0.1 936,147 - RR42_RS04230 0.46 -0.2 936,147 - RR42_RS04230 0.46 -0.1 936,147 - RR42_RS04230 0.46 -1.8 936,248 + RR42_RS04230 0.57 -0.5 936,249 - RR42_RS04230 0.57 -0.5 936,249 - RR42_RS04230 0.57 -0.7
Or see this region's nucleotide sequence