Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04200 and RR42_RS04205 are separated by 66 nucleotides RR42_RS04205 and RR42_RS04210 are separated by 173 nucleotides
RR42_RS04200: RR42_RS04200 - peptidase M48, at 929,729 to 930,889
_RS04200
RR42_RS04205: RR42_RS04205 - glycine cleavage system regulatory protein, at 930,956 to 931,843
_RS04205
RR42_RS04210: RR42_RS04210 - membrane protein, at 932,017 to 933,306
_RS04210
Position (kb) 930
931
932 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 930.086 kb on + strand, within RR42_RS04200 at 930.092 kb on + strand, within RR42_RS04200 at 930.092 kb on + strand, within RR42_RS04200 at 930.093 kb on - strand, within RR42_RS04200 at 930.509 kb on + strand, within RR42_RS04200 at 930.590 kb on + strand, within RR42_RS04200 at 930.591 kb on - strand, within RR42_RS04200 at 930.887 kb on + strand at 930.887 kb on + strand at 930.887 kb on + strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.911 kb on - strand at 930.911 kb on - strand at 930.911 kb on - strand at 930.976 kb on + strand at 930.976 kb on + strand at 930.977 kb on - strand at 930.977 kb on - strand at 930.977 kb on - strand at 931.032 kb on + strand at 931.033 kb on - strand at 931.033 kb on - strand at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.411 kb on - strand, within RR42_RS04205 at 931.954 kb on - strand at 931.972 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.149 kb on - strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.240 kb on - strand, within RR42_RS04210 at 932.352 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 930,086 + RR42_RS04200 0.31 +1.0 930,092 + RR42_RS04200 0.31 -0.3 930,092 + RR42_RS04200 0.31 -0.0 930,093 - RR42_RS04200 0.31 -1.2 930,509 + RR42_RS04200 0.67 +0.2 930,590 + RR42_RS04200 0.74 -0.8 930,591 - RR42_RS04200 0.74 +0.2 930,887 + -0.1 930,887 + -0.1 930,887 + -1.2 930,888 - -1.6 930,888 - +3.3 930,888 - -2.5 930,888 - -0.4 930,910 + -0.2 930,910 + -0.4 930,910 + +1.4 930,910 + +0.8 930,911 - +0.5 930,911 - -0.1 930,911 - +1.3 930,976 + -0.9 930,976 + -0.4 930,977 - +0.6 930,977 - -3.2 930,977 - -1.2 931,032 + +0.9 931,033 - -0.3 931,033 - -0.8 931,093 - RR42_RS04205 0.15 +1.8 931,093 - RR42_RS04205 0.15 +0.7 931,093 - RR42_RS04205 0.15 +0.7 931,411 - RR42_RS04205 0.51 +1.1 931,954 - -0.3 931,972 + -3.5 932,033 + +0.2 932,033 + +0.9 932,033 + +0.1 932,146 + RR42_RS04210 0.10 +0.6 932,146 + RR42_RS04210 0.10 +0.2 932,146 + RR42_RS04210 0.10 +0.4 932,147 - RR42_RS04210 0.10 +0.4 932,147 - RR42_RS04210 0.10 -0.6 932,148 + RR42_RS04210 0.10 -1.0 932,148 + RR42_RS04210 0.10 -0.4 932,148 + RR42_RS04210 0.10 -1.9 932,149 - RR42_RS04210 0.10 +0.5 932,239 + RR42_RS04210 0.17 -0.4 932,239 + RR42_RS04210 0.17 +0.5 932,240 - RR42_RS04210 0.17 -0.3 932,352 + RR42_RS04210 0.26 -0.7 932,581 + RR42_RS04210 0.44 +0.5 932,581 + RR42_RS04210 0.44 -0.1 932,809 + RR42_RS04210 0.61 -0.5 932,809 + RR42_RS04210 0.61 +0.2
Or see this region's nucleotide sequence