Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04140 and RR42_RS04145 are separated by 107 nucleotides RR42_RS04145 and RR42_RS04150 are separated by 64 nucleotides RR42_RS04150 and RR42_RS04155 are separated by 165 nucleotides
RR42_RS04140: RR42_RS04140 - ATPase, at 915,412 to 916,770
_RS04140
RR42_RS04145: RR42_RS04145 - peptidase A24, at 916,878 to 917,426
_RS04145
RR42_RS04150: RR42_RS04150 - pilus assembly protein, at 917,491 to 917,649
_RS04150
RR42_RS04155: RR42_RS04155 - pilus assembly protein PilA, at 917,815 to 918,000
_RS04155
Position (kb) 916
917
918 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 915.896 kb on + strand, within RR42_RS04140 at 915.896 kb on + strand, within RR42_RS04140 at 915.897 kb on - strand, within RR42_RS04140 at 915.897 kb on - strand, within RR42_RS04140 at 915.918 kb on + strand, within RR42_RS04140 at 915.918 kb on + strand, within RR42_RS04140 at 915.919 kb on - strand, within RR42_RS04140 at 915.935 kb on + strand, within RR42_RS04140 at 915.936 kb on - strand, within RR42_RS04140 at 916.125 kb on + strand, within RR42_RS04140 at 916.184 kb on + strand, within RR42_RS04140 at 916.184 kb on + strand, within RR42_RS04140 at 916.185 kb on - strand, within RR42_RS04140 at 916.211 kb on + strand, within RR42_RS04140 at 916.212 kb on - strand, within RR42_RS04140 at 916.302 kb on + strand, within RR42_RS04140 at 916.303 kb on - strand, within RR42_RS04140 at 916.358 kb on + strand, within RR42_RS04140 at 916.392 kb on - strand, within RR42_RS04140 at 916.392 kb on - strand, within RR42_RS04140 at 916.610 kb on - strand, within RR42_RS04140 at 916.992 kb on - strand, within RR42_RS04145 at 917.200 kb on + strand, within RR42_RS04145 at 917.566 kb on + strand, within RR42_RS04150 at 917.657 kb on - strand at 917.657 kb on - strand at 917.809 kb on + strand at 918.152 kb on - strand at 918.247 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 915,896 + RR42_RS04140 0.36 -0.4 915,896 + RR42_RS04140 0.36 +0.6 915,897 - RR42_RS04140 0.36 -2.8 915,897 - RR42_RS04140 0.36 +0.8 915,918 + RR42_RS04140 0.37 -1.0 915,918 + RR42_RS04140 0.37 -2.1 915,919 - RR42_RS04140 0.37 -0.6 915,935 + RR42_RS04140 0.38 -1.6 915,936 - RR42_RS04140 0.39 +1.4 916,125 + RR42_RS04140 0.52 -0.8 916,184 + RR42_RS04140 0.57 -1.5 916,184 + RR42_RS04140 0.57 -0.6 916,185 - RR42_RS04140 0.57 +0.1 916,211 + RR42_RS04140 0.59 -0.2 916,212 - RR42_RS04140 0.59 -2.5 916,302 + RR42_RS04140 0.65 +1.4 916,303 - RR42_RS04140 0.66 +0.1 916,358 + RR42_RS04140 0.70 -0.0 916,392 - RR42_RS04140 0.72 -3.8 916,392 - RR42_RS04140 0.72 -1.2 916,610 - RR42_RS04140 0.88 -1.8 916,992 - RR42_RS04145 0.21 -2.5 917,200 + RR42_RS04145 0.59 -1.3 917,566 + RR42_RS04150 0.47 +2.1 917,657 - -2.6 917,657 - +0.1 917,809 + +0.8 918,152 - +0.9 918,247 + -1.4
Or see this region's nucleotide sequence