Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03955 and RR42_RS03960 are separated by 49 nucleotides RR42_RS03960 and RR42_RS03965 are separated by 186 nucleotides
RR42_RS03955: RR42_RS03955 - amidinotransferase, at 872,611 to 873,510
_RS03955
RR42_RS03960: RR42_RS03960 - ornithine cyclodeaminase, at 873,560 to 874,636
_RS03960
RR42_RS03965: RR42_RS03965 - DNA-binding protein, at 874,823 to 875,425
_RS03965
Position (kb)
873
874
875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 872.653 kb on + strand at 872.653 kb on + strand at 872.654 kb on - strand at 872.654 kb on - strand at 873.139 kb on + strand, within RR42_RS03955 at 873.139 kb on + strand, within RR42_RS03955 at 873.139 kb on + strand, within RR42_RS03955 at 873.140 kb on - strand, within RR42_RS03955 at 873.140 kb on - strand, within RR42_RS03955 at 873.187 kb on + strand, within RR42_RS03955 at 873.188 kb on - strand, within RR42_RS03955 at 873.188 kb on - strand, within RR42_RS03955 at 873.190 kb on + strand, within RR42_RS03955 at 873.396 kb on - strand, within RR42_RS03955 at 873.403 kb on + strand, within RR42_RS03955 at 873.403 kb on + strand, within RR42_RS03955 at 873.403 kb on + strand, within RR42_RS03955 at 873.403 kb on + strand, within RR42_RS03955 at 873.404 kb on - strand, within RR42_RS03955 at 873.404 kb on - strand, within RR42_RS03955 at 873.771 kb on - strand, within RR42_RS03960 at 873.771 kb on - strand, within RR42_RS03960 at 873.782 kb on + strand, within RR42_RS03960 at 873.782 kb on + strand, within RR42_RS03960 at 873.782 kb on + strand, within RR42_RS03960 at 873.782 kb on + strand, within RR42_RS03960 at 873.783 kb on - strand, within RR42_RS03960 at 874.053 kb on - strand, within RR42_RS03960 at 874.237 kb on + strand, within RR42_RS03960 at 874.237 kb on + strand, within RR42_RS03960 at 874.238 kb on - strand, within RR42_RS03960 at 874.571 kb on + strand at 874.571 kb on + strand at 874.571 kb on + strand at 874.706 kb on - strand at 874.773 kb on + strand at 874.773 kb on + strand at 874.774 kb on - strand at 874.774 kb on - strand at 874.774 kb on - strand at 874.948 kb on - strand, within RR42_RS03965 at 874.948 kb on - strand, within RR42_RS03965 at 875.052 kb on + strand, within RR42_RS03965 at 875.052 kb on + strand, within RR42_RS03965 at 875.053 kb on - strand, within RR42_RS03965 at 875.055 kb on + strand, within RR42_RS03965 at 875.056 kb on - strand, within RR42_RS03965 at 875.056 kb on - strand, within RR42_RS03965 at 875.089 kb on + strand, within RR42_RS03965 at 875.089 kb on + strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.099 kb on - strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.339 kb on + strand, within RR42_RS03965 at 875.340 kb on - strand, within RR42_RS03965 at 875.388 kb on + strand at 875.388 kb on + strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.392 kb on - strand at 875.442 kb on + strand at 875.443 kb on - strand at 875.521 kb on + strand at 875.521 kb on + strand at 875.559 kb on - strand at 875.559 kb on - strand at 875.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 872,653 + -0.3 872,653 + -2.0 872,654 - +0.3 872,654 - +0.8 873,139 + RR42_RS03955 0.59 +0.1 873,139 + RR42_RS03955 0.59 -0.2 873,139 + RR42_RS03955 0.59 -1.2 873,140 - RR42_RS03955 0.59 -3.9 873,140 - RR42_RS03955 0.59 -0.2 873,187 + RR42_RS03955 0.64 +2.7 873,188 - RR42_RS03955 0.64 +1.0 873,188 - RR42_RS03955 0.64 +0.2 873,190 + RR42_RS03955 0.64 +0.2 873,396 - RR42_RS03955 0.87 +0.3 873,403 + RR42_RS03955 0.88 -0.2 873,403 + RR42_RS03955 0.88 -2.4 873,403 + RR42_RS03955 0.88 +0.3 873,403 + RR42_RS03955 0.88 -0.2 873,404 - RR42_RS03955 0.88 -1.2 873,404 - RR42_RS03955 0.88 +1.3 873,771 - RR42_RS03960 0.20 -1.1 873,771 - RR42_RS03960 0.20 -1.2 873,782 + RR42_RS03960 0.21 +0.0 873,782 + RR42_RS03960 0.21 -0.6 873,782 + RR42_RS03960 0.21 +0.5 873,782 + RR42_RS03960 0.21 -1.0 873,783 - RR42_RS03960 0.21 +1.2 874,053 - RR42_RS03960 0.46 -0.3 874,237 + RR42_RS03960 0.63 -0.1 874,237 + RR42_RS03960 0.63 +0.8 874,238 - RR42_RS03960 0.63 -2.5 874,571 + +0.2 874,571 + +0.5 874,571 + +0.6 874,706 - -1.2 874,773 + -1.0 874,773 + +1.2 874,774 - +0.2 874,774 - +0.6 874,774 - -1.5 874,948 - RR42_RS03965 0.21 -0.5 874,948 - RR42_RS03965 0.21 -0.1 875,052 + RR42_RS03965 0.38 +0.3 875,052 + RR42_RS03965 0.38 +0.6 875,053 - RR42_RS03965 0.38 -1.2 875,055 + RR42_RS03965 0.38 +0.9 875,056 - RR42_RS03965 0.39 -1.3 875,056 - RR42_RS03965 0.39 -0.0 875,089 + RR42_RS03965 0.44 +0.5 875,089 + RR42_RS03965 0.44 -0.6 875,090 - RR42_RS03965 0.44 -0.7 875,090 - RR42_RS03965 0.44 -1.2 875,090 - RR42_RS03965 0.44 +1.9 875,090 - RR42_RS03965 0.44 +0.7 875,099 - RR42_RS03965 0.46 +0.4 875,272 + RR42_RS03965 0.74 -0.5 875,272 + RR42_RS03965 0.74 -0.1 875,272 + RR42_RS03965 0.74 -0.8 875,273 - RR42_RS03965 0.75 +1.1 875,273 - RR42_RS03965 0.75 -0.4 875,273 - RR42_RS03965 0.75 -0.2 875,273 - RR42_RS03965 0.75 +1.8 875,273 - RR42_RS03965 0.75 -1.2 875,276 + RR42_RS03965 0.75 +1.3 875,276 + RR42_RS03965 0.75 -0.1 875,276 + RR42_RS03965 0.75 -1.4 875,277 - RR42_RS03965 0.75 +0.4 875,277 - RR42_RS03965 0.75 -0.4 875,277 - RR42_RS03965 0.75 +0.3 875,277 - RR42_RS03965 0.75 +0.0 875,277 - RR42_RS03965 0.75 +0.1 875,277 - RR42_RS03965 0.75 -2.0 875,277 - RR42_RS03965 0.75 +0.7 875,277 - RR42_RS03965 0.75 -1.2 875,339 + RR42_RS03965 0.86 +0.6 875,340 - RR42_RS03965 0.86 -1.2 875,388 + +0.7 875,388 + +0.5 875,389 - -0.9 875,389 - +0.9 875,389 - +0.2 875,389 - +0.2 875,389 - +0.2 875,392 - -0.7 875,442 + -0.3 875,443 - -0.9 875,521 + -1.7 875,521 + +0.6 875,559 - -2.8 875,559 - -0.8 875,559 - -2.8
Or see this region's nucleotide sequence