Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03635 and RR42_RS03640 are separated by 61 nucleotides RR42_RS03640 and RR42_RS03645 are separated by 192 nucleotides RR42_RS03645 and RR42_RS03650 are separated by 95 nucleotides RR42_RS03650 and RR42_RS03655 are separated by 215 nucleotides
RR42_RS03635: RR42_RS03635 - trypsin-like serine protease, at 809,187 to 810,089
_RS03635
RR42_RS03640: RR42_RS03640 - hypothetical protein, at 810,151 to 810,435
_RS03640
RR42_RS03645: RR42_RS03645 - hypothetical protein, at 810,628 to 810,951
_RS03645
RR42_RS03650: RR42_RS03650 - hypothetical protein, at 811,047 to 811,331
_RS03650
RR42_RS03655: RR42_RS03655 - hypothetical protein, at 811,547 to 811,921
_RS03655
Position (kb) 810
811 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 809.677 kb on - strand, within RR42_RS03635 at 809.890 kb on - strand, within RR42_RS03635 at 809.890 kb on - strand, within RR42_RS03635 at 810.087 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.118 kb on - strand at 810.175 kb on + strand at 810.176 kb on - strand at 810.192 kb on - strand, within RR42_RS03640 at 810.192 kb on - strand, within RR42_RS03640 at 810.193 kb on + strand, within RR42_RS03640 at 810.193 kb on + strand, within RR42_RS03640 at 810.194 kb on - strand, within RR42_RS03640 at 810.206 kb on - strand, within RR42_RS03640 at 810.206 kb on - strand, within RR42_RS03640 at 810.632 kb on + strand at 810.632 kb on + strand at 810.632 kb on + strand at 810.633 kb on - strand at 810.633 kb on - strand at 810.633 kb on - strand at 810.650 kb on + strand at 810.650 kb on + strand at 810.650 kb on + strand at 810.753 kb on + strand, within RR42_RS03645 at 810.753 kb on + strand, within RR42_RS03645 at 810.753 kb on + strand, within RR42_RS03645 at 810.833 kb on + strand, within RR42_RS03645 at 810.834 kb on - strand, within RR42_RS03645 at 810.834 kb on - strand, within RR42_RS03645 at 810.835 kb on + strand, within RR42_RS03645 at 810.835 kb on + strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.842 kb on + strand, within RR42_RS03645 at 810.843 kb on - strand, within RR42_RS03645 at 810.950 kb on - strand at 811.055 kb on + strand at 811.056 kb on - strand at 811.071 kb on + strand at 811.072 kb on - strand at 811.072 kb on - strand at 811.072 kb on - strand at 811.072 kb on - strand at 811.286 kb on + strand, within RR42_RS03650 at 811.287 kb on - strand, within RR42_RS03650 at 811.287 kb on - strand, within RR42_RS03650 at 811.290 kb on - strand, within RR42_RS03650 at 811.292 kb on + strand, within RR42_RS03650 at 811.329 kb on + strand at 811.330 kb on - strand at 811.330 kb on - strand at 811.359 kb on - strand at 811.415 kb on - strand at 811.495 kb on - strand at 811.541 kb on + strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.614 kb on - strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.799 kb on + strand, within RR42_RS03655 at 811.800 kb on - strand, within RR42_RS03655 at 811.823 kb on + strand, within RR42_RS03655 at 811.824 kb on - strand, within RR42_RS03655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 809,677 - RR42_RS03635 0.54 -0.7 809,890 - RR42_RS03635 0.78 -1.1 809,890 - RR42_RS03635 0.78 +1.2 810,087 + +0.2 810,117 + +0.1 810,117 + -1.7 810,117 + -0.4 810,117 + -0.4 810,118 - +0.1 810,175 + +1.6 810,176 - -1.4 810,192 - RR42_RS03640 0.14 -0.6 810,192 - RR42_RS03640 0.14 -1.4 810,193 + RR42_RS03640 0.15 -0.5 810,193 + RR42_RS03640 0.15 -2.4 810,194 - RR42_RS03640 0.15 +0.7 810,206 - RR42_RS03640 0.19 -0.1 810,206 - RR42_RS03640 0.19 -2.4 810,632 + +0.9 810,632 + +0.3 810,632 + -2.3 810,633 - -0.6 810,633 - +2.5 810,633 - -1.7 810,650 + +0.5 810,650 + +0.7 810,650 + -1.4 810,753 + RR42_RS03645 0.39 +0.0 810,753 + RR42_RS03645 0.39 -0.8 810,753 + RR42_RS03645 0.39 -0.9 810,833 + RR42_RS03645 0.63 +0.5 810,834 - RR42_RS03645 0.64 +0.3 810,834 - RR42_RS03645 0.64 +0.1 810,835 + RR42_RS03645 0.64 +2.0 810,835 + RR42_RS03645 0.64 +0.1 810,836 - RR42_RS03645 0.64 +0.5 810,836 - RR42_RS03645 0.64 +1.0 810,836 - RR42_RS03645 0.64 -1.9 810,836 - RR42_RS03645 0.64 +0.1 810,842 + RR42_RS03645 0.66 +0.9 810,843 - RR42_RS03645 0.66 +0.6 810,950 - -1.0 811,055 + +0.7 811,056 - +1.1 811,071 + -2.2 811,072 - +0.1 811,072 - +0.5 811,072 - -1.2 811,072 - +1.6 811,286 + RR42_RS03650 0.84 -1.7 811,287 - RR42_RS03650 0.84 +0.0 811,287 - RR42_RS03650 0.84 -2.8 811,290 - RR42_RS03650 0.85 -0.6 811,292 + RR42_RS03650 0.86 -1.5 811,329 + -2.2 811,330 - -1.4 811,330 - -1.1 811,359 - +0.5 811,415 - +0.4 811,495 - +0.1 811,541 + -0.7 811,542 - -0.6 811,542 - +0.6 811,542 - -0.2 811,542 - +0.7 811,542 - -0.2 811,614 - RR42_RS03655 0.18 -0.3 811,620 + RR42_RS03655 0.19 +1.1 811,620 + RR42_RS03655 0.19 +1.0 811,620 + RR42_RS03655 0.19 -0.7 811,621 - RR42_RS03655 0.20 +0.3 811,621 - RR42_RS03655 0.20 +0.1 811,621 - RR42_RS03655 0.20 +1.4 811,799 + RR42_RS03655 0.67 +0.6 811,800 - RR42_RS03655 0.67 -2.3 811,823 + RR42_RS03655 0.74 -0.8 811,824 - RR42_RS03655 0.74 -1.5
Or see this region's nucleotide sequence