Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03405 and RR42_RS03410 are separated by 16 nucleotides RR42_RS03410 and RR42_RS03415 overlap by 7 nucleotides RR42_RS03415 and RR42_RS03420 are separated by 70 nucleotides
RR42_RS03405: RR42_RS03405 - epoxyqueuosine reductase, at 757,589 to 758,809
_RS03405
RR42_RS03410: RR42_RS03410 - ATPase, at 758,826 to 759,338
_RS03410
RR42_RS03415: RR42_RS03415 - N-acetylmuramoyl-L-alanine amidase, at 759,332 to 760,837
_RS03415
RR42_RS03420: RR42_RS03420 - bile acid:sodium symporter, at 760,908 to 761,930
_RS03420
Position (kb) 759
760
761 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 758.349 kb on + strand, within RR42_RS03405 at 758.452 kb on - strand, within RR42_RS03405 at 758.533 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.820 kb on + strand at 758.820 kb on + strand at 758.820 kb on + strand at 759.506 kb on + strand, within RR42_RS03415 at 759.506 kb on + strand, within RR42_RS03415 at 759.507 kb on - strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.745 kb on + strand, within RR42_RS03415 at 759.746 kb on - strand, within RR42_RS03415 at 759.794 kb on + strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.318 kb on - strand, within RR42_RS03415 at 760.793 kb on + strand at 760.793 kb on + strand at 760.794 kb on - strand at 760.794 kb on - strand at 760.836 kb on - strand at 760.999 kb on + strand at 761.000 kb on - strand at 761.227 kb on + strand, within RR42_RS03420 at 761.227 kb on + strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.272 kb on + strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.427 kb on - strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 758,349 + RR42_RS03405 0.62 +1.5 758,452 - RR42_RS03405 0.71 -0.2 758,533 + RR42_RS03405 0.77 -0.4 758,535 + RR42_RS03405 0.77 -0.3 758,535 + RR42_RS03405 0.77 -0.7 758,535 + RR42_RS03405 0.77 -1.2 758,535 + RR42_RS03405 0.77 -2.0 758,535 + RR42_RS03405 0.77 +1.6 758,820 + -0.2 758,820 + -0.2 758,820 + +1.5 759,506 + RR42_RS03415 0.12 +2.6 759,506 + RR42_RS03415 0.12 +2.2 759,507 - RR42_RS03415 0.12 -2.2 759,656 + RR42_RS03415 0.22 +0.0 759,656 + RR42_RS03415 0.22 +2.8 759,657 - RR42_RS03415 0.22 +1.0 759,657 - RR42_RS03415 0.22 +0.3 759,745 + RR42_RS03415 0.27 +2.8 759,746 - RR42_RS03415 0.27 +1.5 759,794 + RR42_RS03415 0.31 +0.2 760,268 - RR42_RS03415 0.62 +1.4 760,268 - RR42_RS03415 0.62 +1.0 760,318 - RR42_RS03415 0.65 +1.6 760,793 + -1.4 760,793 + +1.9 760,794 - +3.8 760,794 - -1.6 760,836 - -0.1 760,999 + -0.1 761,000 - -0.8 761,227 + RR42_RS03420 0.31 -2.3 761,227 + RR42_RS03420 0.31 -1.2 761,228 - RR42_RS03420 0.31 -3.4 761,228 - RR42_RS03420 0.31 -0.6 761,228 - RR42_RS03420 0.31 +0.2 761,272 + RR42_RS03420 0.36 +0.9 761,273 - RR42_RS03420 0.36 +0.6 761,273 - RR42_RS03420 0.36 +0.7 761,426 + RR42_RS03420 0.51 +0.1 761,426 + RR42_RS03420 0.51 +0.8 761,427 - RR42_RS03420 0.51 -1.9 761,590 + RR42_RS03420 0.67 -0.4 761,590 + RR42_RS03420 0.67 +0.3 761,590 + RR42_RS03420 0.67 -0.8 761,591 - RR42_RS03420 0.67 +0.3 761,591 - RR42_RS03420 0.67 -0.4 761,591 - RR42_RS03420 0.67 -0.0 761,591 - RR42_RS03420 0.67 -0.8 761,591 - RR42_RS03420 0.67 -1.5 761,674 + RR42_RS03420 0.75 -0.8 761,674 + RR42_RS03420 0.75 +0.6 761,674 + RR42_RS03420 0.75 +0.5 761,674 + RR42_RS03420 0.75 -0.8 761,674 + RR42_RS03420 0.75 +0.2 761,675 - RR42_RS03420 0.75 -1.2 761,675 - RR42_RS03420 0.75 +0.8 761,675 - RR42_RS03420 0.75 -0.4 761,675 - RR42_RS03420 0.75 +0.4
Or see this region's nucleotide sequence